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  • 1
    Digitale Medien
    Digitale Medien
    Saccharide production from methanol by transposon 5 mutants derived from the extracellular polysaccharide-producing bacterium Methylobacillus sp. strain 12S (2000)
    Springer
    Applied microbiology and biotechnology 54 (2000), S. 341-347 
    Details
    ISSN: 1432-0614
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract A CH3OH-utilizing bacterium that has the ability to produce extracellular polysaccharide (EPS) was isolated from a soil sample, and was identified as the obligate methylotroph Methylobacillus sp. strain 12S on the basis of its 16S rDNA sequence and growth-substrate specificity. The EPS produced by strain 12S was purified and the sugar composition was analysed by GC-MS and HPLC to reveal that the EPS was a heteropolymer composed of glucosyl, galactosyl, and mannosyl residues in the molar ratio 3:1:1. In order to produce mono- and/or oligosaccharides by single-step fermentation from CH3OH, stain 12S was mutagenized by transposon 5. Among eleven EPS-deficient mutants, three strains were found to accumulate significant amounts of reducing sugars in the media. The amounts of the reducing sugars produced by the mutants (〉ca. 700 mg glucose equivalent/l) were 〉11–22 times higher than those produced by the wild-type strain (〈ca. 60 mg glucose equivalent/l). The GC-MS analysis showed that all the mutants accumulated glucose, erythrose, threose and a disaccharide-like compound in the media.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002530000407
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  • 2
    Digitale Medien
    Digitale Medien
    Model of neural visual system with self-organizing cells (1989)
    Springer
    Biological cybernetics 60 (1989), S. 195-202 
    Details
    ISSN: 1432-0770
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Informatik , Physik
    Notizen: Abstract This paper describes a model of a neural visual system of a higher animal, in which the capability of pattern recognition develops adaptively. To produce the adaptability, we adopted “self-organizing cells,” and with them modeled feature-detecting cells which were discovered by Hubel and Wiesel and whose plasticity was found by Blakemore and Cooper. Combining the “self-organizing cells” and the learning principle of a Perceptron-type system, we constructed a model of the whole visual system. The model is also equipped with an eye movement control mechanism for gazing, which reduces the number of “selforganizing cells” required for pattern recognition, thus contributing to their quick self-organization. Computer simulation and an experiment using a hardware simulator showed that “self-organizing cells” quickly become sensitive to the features often seen and that the resulted system can classify patterns with a rather small number of feature-detecting cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00207287
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  • 3
    Digitale Medien
    Digitale Medien
    Column chromatography of carrier-free Tc/III, IV/-TTA complexes (1984)
    Springer
    Journal of radioanalytical and nuclear chemistry 85 (1984), S. 245-251 
    Details
    ISSN: 1588-2780
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Energietechnik
    Notizen: Abstract The chemical species of carrier-free technetium, which were extractable into a TTA-benzene solution on reduction of96TcO 4 − either with NaBH4 or concentrated HCl, were studied by means of silica gel chromatography. Elution peaks ascribed to the formation of Tc/tta/4 and Tc/tta/3 type complexes were observed. The latter complex was also synthesized by recoil effect of Ru/γ, p/Tc reactions in γ-irradiated Ru/tta/3.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02164229
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  • 4
    Digitale Medien
    Digitale Medien
    Application of proton induced X-ray emission to the qualitative and quantitative analysis of iodine in biological samples (1985)
    Springer
    Journal of radioanalytical and nuclear chemistry 91 (1985), S. 163-171 
    Details
    ISSN: 1588-2780
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Energietechnik
    Notizen: Abstract Particle-induced X-ray emission (PIXE) was applied to evaluate the loss of volatile elements such as iodine in biological samples. The analytical quality of the method is comparable or better than spectrophotometry, which is currently believed to be the most reliable for iodine determination. The temperature dependence of volatility loss of trace iodine was characteristic, and the feature was divided into three temperature regions. The first one, ranging from room temperature to 200 °C, showed only a slight loss below 20% on drying; the second stage, between 200 °C and 350 °C, where carbonizing processes became prominent, showed a remarkable loss up to 50%; the last one, beyond 350 °C, was accompanied by a considerable loss of iodine (more than 80%) with ashing. Even in the analysis using low temperature ashing with oxygen plasma, the loss of iodine observed was considerable (nearly 80%). The significance of these findings by PIXE in trace analysis is noted to improve analytical quality of volatile elements, such as iodine in biological, medical and also environmental fields.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02036322
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  • 5
    Digitale Medien
    Digitale Medien
    Evaluation of phenyldimethylethoxysilane treated high-performance thin-layer chromatographic plates. Application to analysis of flavonoids inScutellariae radix (1993)
    Springer
    Chromatographia 35 (1993), S. 281-284 
    Details
    ISSN: 1612-1112
    Schlagwort(e): Thin-layer chromatography ; Monophenyl-treated thin layer plate ; Phenyldimethylethoxysilane treated plate ; Flavonoids inScutellariae radix ; Baicalin
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Summary The retention and selectivity of flavonoids (baicalin, baicalein, wogonin, oroxylin A) inScutellariae radix have been studies by high-performance thin-layer chromatography on phenyldimethylethoxysilane-treated silica plates. The silica plates treated with phenyl groups were used for physical and chemical analysis. From elemental carbon analysis, the maximum number of bonded phenyl surface groups per gram was calculated to be 0.467×1021 (Oginal silica plate: Merck Art. 15109, Silica gel 100 F254). With methanol-1/15 M phosphate buffer (pH 6.2) mixtures as mobile phase, baicalin, baicalein, wogonin, and oroxylin A inScutellariae radix were separated. It has been shown that phenyl-treated plates are more suitable for selective separation of baicalin, baicalein, wogonin, and oroxylin A than octadecyl-treated plates.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02277510
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  • 6
    Digitale Medien
    Digitale Medien
    Chemical effects of L X-rays emitted from iodine and antimony compounds (1994)
    Springer
    Hyperfine interactions 84 (1994), S. 433-437 
    Details
    ISSN: 1572-9540
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract L X-ray spectra of iodine and antimony compounds were measured using a doublecrystal spectrometer, LΥ4 X-rays emitted from the valence 5p electrons were found to be sensitive to the chemical environment. The LΥ4/Lβ3, LΥ1/LΥ1 and Lβ2,15/Lα1 intensity ratios were remarkably changed in various compounds of these elements. LΥ4 energies of iodine compounds were shifted with the increase of the formal oxidation number.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02060693
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  • 7
    Digitale Medien
    Digitale Medien
    A new internal standard method coupled with the standard addition method for proton-induced X-ray emission analysis and its application determination of Cu, Zn, Rb and Sr in biological materials (1987)
    Springer
    Journal of radioanalytical and nuclear chemistry 116 (1987), S. 213-222 
    Details
    ISSN: 1588-2780
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Energietechnik
    Notizen: Abstract A new internal standard method coupled with the standard addition method for PIXE analysis has been developed. In its basic principle, this method is characterized by that a suitable element present initially in the sample is used as an internal standard, and further the comparative standard is prepared by applying the standard addition method to the duplicated sample. When a sample contains Wa g of trace element A to be determined together with an element B which is usable as an internal standard, and when the comparative standard is prepared by adding an exactly known amount of element A, W a * g, to the duplicated sample, the absolute concentration of Wa can easily be determined by the following equation even if the above sample and comparative standard are irradiated separately by a different number of protons Wa=W a * /[(R*/R)-1] where R and R* are ratios of net photopeak counts due to the characteristic X-rays from the element A and B in the irradiated sample and comparative standard, respectively. The usefulness of the method was examined through determination of Cu, Zn, Rb and Sr in two biological materials, such as spinach and orchard leaves. As a result, this method was demonstrated to be sensitive, highly reproducible and reasonably accurate.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02037224
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  • 8
    Digitale Medien
    Digitale Medien
    Chemical effects on Lγ1/Lβ1 X-ray intensity ratio of molybdenum compounds (1987)
    Springer
    Journal of radioanalytical and nuclear chemistry 118 (1987), S. 59-67 
    Details
    ISSN: 1588-2780
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Energietechnik
    Notizen: Abstract Chemical effects on the intensity ratio of LX-ray of molybdenum compounds irradiated by 11-keV electrons and by 3-MeV protons were studied using an X-ray crystal spectrometer. It was found that the intensity ratios of Lγ1/Lβ1 markedly decrease with increase of ionicity of molybdenum compounds. An exception was for the case of metallic molybdenum.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02165654
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  • 9
    Digitale Medien
    Digitale Medien
    Base hydrolysis of halobis/8-quinolinolato/ oxotechnetium/V/ complexes (1987)
    Springer
    Journal of radioanalytical and nuclear chemistry 119 (1987), S. 223-233 
    Details
    ISSN: 1588-2780
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Energietechnik
    Notizen: Abstract Reaction mechanism of base hydrolysis of halobis/8-quinolinolato/oxotechnetium (V) /TcOX (ox)2′ X=Cl and Br/ was investigated by means of solvent extraction and spectrophotometric methods. Furthermore, distribution coefficients of tris/acetylacetonato/technetium/III/, dichlorobis/8-quinolinolato/technetium/IV/ and TcOX/ox/2 between chloroform and aqueous solutions were determined. In an alkaline solution, TcOX/ox/2 decomposed to pertechnetate as a final product. On the basis of the established base hydrolysis, the respective rate constants were determined.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02162267
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  • 10
    Digitale Medien
    Digitale Medien
    Synthesis of chlorobis/β-diketonato/oxotechnetium/V/ and tris/β-diketonato/technetium/III/ complexes (1989)
    Springer
    Journal of radioanalytical and nuclear chemistry 135 (1989), S. 187-197 
    Details
    ISSN: 1588-2780
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Energietechnik
    Notizen: Abstract Chlorobis/β-diketonato/ oxotechnetium/V/ complexes [TcOCl/β-dik/2, β-diketone=acetylacetone, benzoylacetone and dibenzoylmethane] were newly synthesized using macroamount of99Tc. These complexes were further separated into geometrical isomers. Furthermore, an improvement of the yields for the syntheses of tris/β-dike-tonato/technetium/III/ complexes [Tc/β-dik/3, β-diketone=acetylacetone, benzoylacetone and 2-thenoyltrifluoroacetone] was examined using Tc/III/-thiourea complexes as a starting material.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02164971
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