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  • 1
    Electronic Resource
    Electronic Resource
    Group actions, gauge transformations, and the calculus of variations (1992)
    Springer
    Mathematische Annalen 293 (1992), S. 595-621 
    Details
    ISSN: 1432-1807
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01444737
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  • 2
    Electronic Resource
    Electronic Resource
    UBC13, a DNA-damage-inducible gene, is a member of the error-free postreplication repair pathway in Saccharomyces cerevisiae (2000)
    Springer
    Current genetics 37 (2000), S. 168-174 
    Details
    ISSN: 1432-0983
    Keywords: Key words Yeast ; Postreplication repair ; UBC13 ; Epistatic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Ubc13 protein was recently identified for its unique role in ubiquitin (Ub) chain assembly at the Ub Lys-63 residue instead of the conventional Lys-48 residue. This activity requires Ubc13 to form a complex with Mms2 and indeed ubc13 and mms2 mutations have been shown elsewhere to be epistatic with respect to UV sensitivity. The MMS2 gene is known to be a member of the error-free DNA postreplication repair (PRR) pathway. By contrast, the Ub Lys-63 residue has been previously implicated in the error-prone PRR pathway, since yeast cells carrying the ubiK63R mutation are defective in UV-induced mutagenesis. In the present study, we attempted to define the role of UBC13 within the PRR pathway. We found that the ubc13 mutation is epistatic to mms2 and rad6, confirming that UBC13 belongs to the PRR-pathway. We also found that ubc13 is synergistic to the error-prone PRR pathway mutation rev3, indicating that UBC13 is in a pathway alternative to REV3 mutagenesis. The ubc13 mutant displays up to a 30-fold increase in the spontaneous mutation rate, and this increase is largely REV3 dependent. In addition, UV-induced mutagenesis is fully functional in the ubc13 mutant. These results together demonstrate that UBC13 is a member of the error-free PRR pathway. The involvement of UBC13 in cellular tolerance to DNA-damage is further implicated by our finding that the UBC13 transcript level is increased up to 6-fold in response to DNA-damage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050515
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  • 3
    Electronic Resource
    Electronic Resource
    An experimental analysis of displacement field of mixed mode crack (1988)
    Springer
    Acta mechanica solida Sinica 1 (1988), S. 315-323 
    Details
    ISSN: 0894-9166
    Keywords: moire interferometry ; displacement field of a crack
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract In this paper, the characteristic properties ofv (y-direction displacement) field surrounding the tip of a mixed mode crack are studied. These properties can be used to evaluate the rigid body rotation of the crack tip, theK I SIF and the ratio ofK II SIF toK I. The authors employ a film to record the displacement information based on the technique of moire interferometry with sticking films. By using the data taken from the moire pattern and treating them with the damping least square method, all of the parameters of the crack can be obtained accurately.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02207261
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  • 4
    Electronic Resource
    Electronic Resource
    Generation of an ilv bradytrophic phenocopy in yeast by antisense RNA (1988)
    Springer
    Current genetics 13 (1988), S. 283-289 
    Details
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Inducible antisense gene ; Acetolactate synthase ; Bradytrophic phenocopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We report for the first time on the regulation of gene expression in yeast by antisense RNA. Chimaeric genes were constructed containing the 5′ upstream and partial coding sequence of SMR1 — a sulfometuron methyl resistant allele of the ILV2 locus. Such fragments were placed 5′ to 3′ and 3′ to 5′ under control of the GAL10 promoter and CYCl terminator in a high copy YEp plasmid. Following galactose induction only transformants containing antisense RNA genes showed biological activity against SMR1 gene expression. Antisense RNA inhibited synthesis of the SMR1 gene product acetolactate synthase and thus repressed cellular growth which resulted in a bradytrophic auxotroph revertable by addition of isoleucine and valine. Antisense RNA inhibition was enhanced in galactose medium containing sulfometuron methyl and in gcn4 cells deficient for positive regulation of the ILV2 locus. This system can be used to study factors that interfere with antisense RNA function and to assign biological function to randomly cloned DNA fragments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00424421
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  • 5
    Electronic Resource
    Electronic Resource
    Binding of a synthetic targeting peptide to a mitochondrial channel protein (1992)
    Springer
    Journal of bioenergetics and biomembranes 24 (1992), S. 55-61 
    Details
    ISSN: 1573-6881
    Keywords: Mitochondrial channels ; mitochondrial targeting sequences ; electron microscopy ; computer image processing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract Membrane crystals of the mitochondrial outer membrane channel VDAC (porin) fromNeurospora crassa were incubated with a 20-amino-acid synthetic peptide corresponding to the N-terminal targeting region of subunit IV of cytochrome oxidase. The peptide caused disordering and contraction of the crystal lattice of the membrane arrays. Also, new stain-excluding features were observed on the peptide-treated arrays which most likely correspond to sites at which the peptide accumulates. The stain exclusion zones associated with binding of the targeting peptide (and with binding of apocytochromec in an earlier study) have been localized on a two-dimensional density map of frozen-hydrated, crystalline VDAC previously obtained by cryo-electron microscopy. The results indicate that both the peptide and cytochromec bind to protein “arms” which extend laterally between the channel lumens. The finding that imported polypeptides bind to a specific region of the VDAC protein implicates this channel in the process by which precursor proteins are recognized at and translocated across the mitochondrial outer membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00769531
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  • 6
    Electronic Resource
    Electronic Resource
    Almost negatively Ricci curved metrics onS n (1990)
    Springer
    Archiv der Mathematik 54 (1990), S. 184-192 
    Details
    ISSN: 1420-8938
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01198116
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  • 7
    Electronic Resource
    Electronic Resource
    On the morse complex (1984)
    Springer
    Mathematische Zeitschrift 187 (1984), S. 89-96 
    Details
    ISSN: 1432-1823
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01163169
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  • 8
    Electronic Resource
    Electronic Resource
    Collapsing Riemannian manifolds to the circle (1989)
    Springer
    Mathematische Zeitschrift 202 (1989), S. 289-298 
    Details
    ISSN: 1432-1823
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01215261
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  • 9
    Electronic Resource
    Electronic Resource
    Polymorphism of 2-μm plasmids in industrial strains of Saccharomyces cerevisiae (1993)
    Springer
    Applied microbiology and biotechnology 39 (1993), S. 80-86 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Restriction fragment length polymorphism (RFLP) analyses of industrial Saccharomyces yeast DNA have identified eight 2-μm plasmidsvariants that fall into two distinct types. Type-I plasmids are of unique form, whereas type-II plasmids exist in seven distinct RFLP forms. Only two different 2-μm variants were observed in 35 bakers' strains analysed. One variant was the unique type-I whereas the second variant represents an ancestral form of the type-II plasmid. Sixteen of nineteen wine yeasts carried a distinctive type-II plasmid with a homeologous STB repeat whereas ale and lager yeasts had a wide range of type-II variants. Relative to nuclear and mtDNA, 2-μm polymorphism is less diverse and not diagnostic for a specific strain. This 2-μm DNA polymorphism is a convenient and useful addendum to nuclear and mtDNA RFLP analyses but cannot serve as the sole marker for strain identification. A tentative phylogeny of industrial S. cerevisiae yeasts is suggested with origins in bakers' yeast carrying the ancestral type-II form.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00166853
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  • 10
    Electronic Resource
    Electronic Resource
    The 130 K High-T c superconductor in the system Bi-Sr-Ca-Cu-O doped with Sb and Pb (1990)
    Springer
    Journal of superconductivity 3 (1990), S. 183-187 
    Details
    ISSN: 1572-9605
    Keywords: BiSrCaCuO ; 130 K superconductor ; Sb and Pb doping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Physics
    Notes: Abstract A nearly pure 2223 phase of the ceramic superconductor Bi1.8Pb0.3Sb0.1Sr2Ca2Cu3O10−δ was obtained by solid-state reaction in air. The samples showed a semiconducting behavior when the temperature was higher than 140 K. The electrical resistance began to drop at 140 K and went to zero at 92 K. Their a.c. magnetic susceptibility showed two transitions at 130 and 89 K, but the 110 K transition was absent. These transitions were stable during cycling for three times from 77 K to room temperature. X-ray diffraction revealed that there was no 2212 or 2201 phase in these samples but only a small amount of monoclinic phase before and after cycling from 77 K to room temperature. SEM images showed that the grains of the monoclinic phase segregated on the surface of the sample and at the grain boundaries inside the sample, which is the reason for the sample's poor superconductivity and the decrease in zero resistance. Evidence from TEM and SEM equipped with a wavelength dispersive meter indicated distortion of the 2223 phase. These distorted 2223 crystals may be responsible for the 130 K superconductivity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00624506
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