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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of environmental contamination and toxicology 39 (2000), S. 480-485 
    ISSN: 1432-0703
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Notes: Abstract This study investigated the use of lysosomal responses of hemocytes of the common garden snail, Helix aspersa, as biomarker of stress due to exposure to the fungicide copper oxychloride. The neutral red retention (NRR) time assay was employed for this purpose. Two groups of snails were exposed to 80 μg g−1 and 240 μg g−1 copper oxychloride in their food, respectively, for a period of 6 weeks. They were compared with a control group to which no copper oxychloride was added. The two groups exposed to the fungicide exhibited significantly higher (p 〈 0.001) whole body copper concentrations (200.85 ± 53.5 and 272.24 ± 67.15 μg g−1 dry mass, respectively), and significantly shorter (p 〈 0.001) NRR times (10.22 ± 3.53 and 2.67 ± 2.83 min, respectively), after 6 weeks, compared to the control group (67.85 ± 31.08 μg g−1 dry mass and 24.44 ± 8.35 min). In both exposure groups NRR times became progressively shorter as body copper concentrations increased over time. Thus, both exposure concentration and exposure time of copper oxychloride were shown to be important factors influencing lysosomal responses (and therefore NRR times) of H. aspersa hemocytes. It was concluded that these responses in this species, as measured by the NRR time assay, could be considered a useful cellular biomarker of stress resulting from exposure to copper oxychloride.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of environmental contamination and toxicology 39 (2000), S. 392-397 
    ISSN: 1432-0703
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Notes: Abstract Shrews (Myosorex varius) were bait-trapped close to Stellenbosch in the Western Cape, South Africa, and analyzed to determine the Pb content of the liver, kidneys, muscle tissue, brain, skull, and bone. Shrews were also fed in the laboratory on mince meat and Pb-contaminated live earthworms for 12–17 days and the Pb content compared with control shrews from the field. The Pb levels in the control shrews from the field were exceptionally high and exceeded levels generally considered to be critical for the onset of pathological symptoms in target organs. The shrews that were fed with Pb-contaminated earthworms had significantly higher Pb concentrations in their livers and kidneys than the control shrews. Given that Pb is bioaccumulated in earthworms, this study showed that predation on earthworms could constitute a major pathway for the entry of Pb into the food chain. M. varius could, similar to other species of shrews in other parts of the world, be a suitable biological monitor to study the environmental hazard of Pb pollution.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 24 (1996), S. 27-31 
    ISSN: 1432-0789
    Keywords: Key wordsEudrilus eugeniae ; Toxicity ; Copper ; Zinc ; Growth ; Maturation ; Uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Growth rate change in earthworms is considered to be a suitable endpoint when determining sublethal effects. In this study we evaluated growth and maturation in the vermicomposting earthworm species Eudrilus eugeniae as marker of sublethal toxicity of copper and zinc. We also compared routes of uptake. Apart from exposing worms experimentally for 73 days to contaminated food, a series of contact filter paper tests was also performed to determine LD50 for copper and zinc. Both copper and zinc at sublethal concentrations affected growth and maturation in worms exposed to contaminated food. These worms had a copper content of 34.5 μg g–1 after 73 days and a zinc content of 184.9 μg g–1, showing a differential uptake. Copper was more toxic than zinc. Also in the contact test worms did take up more zinc than copper and the LD50 (48 h) for copper was 0.011 mg cm–2 and for zinc 0.066 mg cm–2, which translated to body burdens of 6 μg g–1 for copper and 131 μg g–1 for zinc. Indications were that a regulatory mechanism existed for both metals. Both metals were taken up through the body wall at a relatively fast rate. This study indicated that the skin was the major route of metal uptake. This study also showed a poor relation between the two types of tests for purposes of evaluating lethality of zinc and copper.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 3 (1987), S. 135-141 
    ISSN: 1432-0789
    Keywords: Eisenia fetida ; Moisture preference ; Growth ; Cocoon production ; Compost worm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Although moisture requirements of Eisenia fetida are considered to be generally known, very few in-depth studies have been performed. The moisture preferences of juveniles as well as adults of this species were determined in moisture towers containing cow manure with a particle size bigger than 500 and smaller than 1000 μm and an organic matter content of 54%. The clitellate worms showed a wider preference range than the juveniles but deposited most of their cocoons within the narrow range of 65%–70% moisture — a moisture range preferred by 80% of the juveniles. Growth studies of newly hatched worms at various moisture levels over a period of 90 days showed that growth rate and final biomass were influenced by moisture availability with a very low rate of growth at low as well as at high moisture levels. The change in biomass of clitellate specimens as well as their consecutively produced cocoons were monitored at different moisture levels. A clear relationship between mass of cocoons and biomass of the producing worms was established at presumably favourable moisture conditions. Furthermore, the relation between cocoon mass and the production rate of cocoons was also determined, which showed that cocoon mass increased concomitantly with increase in cocoon production. The study also showed that clitellum development was increased at moisture levels above 64%. The onset of cocoon production was, however, earliest at slightly lower moisture levels, from which it is concluded that the optimal moisture level for growth and development of clitella are not necessarily optimal for the production of cocoons.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 7 (1988), S. 23-27 
    ISSN: 1432-0789
    Keywords: Eudrilus eugeniae ; Earthworm ; Cocoons ; Incubation ; Reproduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Although various authors have contributed to our knowledge of the biology of Eudrilus eugeniae some basic facts about the life cycle and reproduction are still not fully documented. Quantitative observations were made of the cocoons at 25 °C in different substrates. The incubation period for 166 cocoons was 16.89 days, based on cocoons produced by worms between the ages of 70 and 100 days. These cocoons produced a mean 2.12 hatchlings per cocoon after incubation in cattle manure, moist filter paper and distilled water. A smaller batch of cocoons incubated in cattle manure produced a mean of 2.7 hatchlings per cocoon. The hatching success of the cocoons was 84% in cattle manure, 50% in distilled water, and 48% on moist filter paper. The reproductive capabilities of E. eugeniae and Eisenia fetida were compared.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Archives of environmental contamination and toxicology 36 (1999), S. 64-69 
    ISSN: 1432-0703
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Notes: Abstract. This study investigated the sublethal effects of CdSO4 on the terrestrial isopod species Porcellio laevis and its capacity to accumulate and compartmentalize cadmium. The ability of P. laevis to discriminate between uncontaminated and CdSO4-contaminated leaves was also studied. It was shown that sublethal effects in terms of mass changes of exposed isopods occur at cadmium concentrations between 10 and 80 mg/kg. Moreover, P. laevis can be considered as a so-called macroconcentrator, accumulating and storing cadmium in its hepatopancreas tissue. However, the accumulation capacity is not proportional to the cadmium concentrations in the leaves presented as food, as shown by decreasing concentration factors with increasing cadmium concentrations in the diet. This is probably an effect of the isopod's ability to discriminate between different cadmium concentrations in the food, as shown by means of feed-choice experiments, in which P. laevis was able to avoid contaminated leaves.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 24 (1997), S. 27-31 
    ISSN: 1432-0789
    Keywords: Eudrilus eugeniae ; Toxicity ; Copper ; Zinc Growth ; Maturation ; Uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Growth rate change in earthworms is considered to be a suitable endpoint when determining sublethal effects. In this study we evaluated growth and maturation in the vermicomposting earthworm speciesEudrilus eugeniae as marker of sublethal toxicity of copper and zinc. We also compared routes of uptake. Apart from exposing worms experimentally for 73 days to contaminated food, a series of contact filter paper tests was also performed to determine LD50 for copper and zinc. Both copper and zinc at sublethal concentrations affected growth and maturation in worms exposed to contaminated food. These worms had a copper content of 34.5 μg g−1 after 73 days and a zinc content of 184.9 μg g−1, showing a differential uptake. Copper was more toxic than zinc. Also in the contact test worms did take up more zinc than copper and the LD50 (48 h) for copper was 0.011 mg cm−2 and for zinc 0.066 mg cm−2, which translated to body burdens of 6 μg g−1 for copper and 131 μg g−1 for zinc. Indications were that a regulatory mechanism existed for both metals. Both metals were taken up through the body wall at a relatively fast rate. This study indicated that the skin was the major route of metal uptake. This study also showed a poor relation between the two types of tests for purposes of evaluating lethality of zinc and copper.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 29 (1999), S. 146-151 
    ISSN: 1432-0789
    Keywords: Key words Lead ; Woodlouse ; Porcellio laevis ; Toxicity ; Avoidance behaviour
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  The effects of sublethal concentrations of lead nitrate on the terrestrial isopod Porcellio laevis were studied experimentally under controlled conditions over a period of 8 weeks. Changes in body mass were recorded. Avoidance-behaviour studies were undertaken to determine whether P. laevis can detect and possibly avoid lead in the environment. P. laevis was affected negatively by the presence of lead nitrate and showed a loss in body mass. More than 86% of the total body burden of lead was compartmentalized in the hepatopancreas. The isopods were capable of avoiding leaves contaminated with 200 mg kg–1 lead nitrate in behavioural tests but did not avoid leaves contaminated with a higher concentration.
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  • 9
    ISSN: 1432-0789
    Keywords: Key words Earthworms ; Eudrilus eugeniae ; Lead accumulation ; Growth and reproduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  The effects of sublethal concentrations of lead nitrate on the growth and reproduction of the African composting earthworm species, Eudrilus eugeniae, was studied by exposing worms in an organic substrate to lead-nitrate-contaminated food over a period of 76 days. The results revealed that growth was initially affected negatively by the presence of lead, while the maturation rate and cocoon production were not affected. In agreement with other studies on Eisenia fetida, cocoon viability was affected negatively by lead, making this a sensitive toxicity endpoint.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 8 (1989), S. 303-306 
    ISSN: 1432-0789
    Keywords: Perionyx excavatus ; Earthworm ; Cocoon production ; Growth ; Cattle manure ; Vermicomposting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The biology of the oriental vermicomposting species, Perionyx excavatus, is poorly understood. Quantitative observations were made at 25°C in urine free cattle manure in order to study the growth of this species. The rate of maturation and cocoon production were studied under conditions of favourable moisture and nutrition over a period of 250 days. The results obtained showed that while P. excavatus grows relatively slowly compared to other vermicomposting species, it attains sexual maturity much earlier and maintains a relatively high cocoon production rate. The study revealed that copulation is not a prerequisite for production of viable cocoons, indicating that P. excavatus may be parthenogenetic and possibly capable of alternating between modes of reproduction.
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