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  • 1
    Electronic Resource
    Electronic Resource
    Cryopreservation of zygotic embryos of a Japanese terrestrial orchid (Bletilla striata) by vitrification (1997)
    Springer
    Plant cell reports 16 (1997), S. 754-757 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsBletilla striata ; Cryopreservation ; Embryo ; Orchid ; Vitrification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The seeds of a Japanese terrestrial orchid (Bletilla striata Rchb.f.) were germinated and cultured on solidified new Dogashima (ND) medium for 10 days. These embryos were then precultured on ND medium supplemented with 0.3 m sucrose for 3 days at 25°C in continuous dark. The embryos were then overlaid with a mixture of 2 m glycerol and 0.4 m sucrose for 15 min at 25°C and finally dehydrated with highly concentrated vitrification solution (PVS2) for 3 h at 0°C prior to immersion into liquid nitrogen for 30 min. After rapid warming, the embryos were washed with liquid ND medium supplemented with 1.2 m sucrose for 20 min and then plated on ND medium. Successfully vitrified and warmed embryos developed into normal plantlets. The rate of plant regeneration amounted to about 60%. This vitrification method appears to be a promising technique for cryopreservation of orchids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050314
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  • 2
    Electronic Resource
    Electronic Resource
    A simple and efficient plant regeneration system for leaf protoplasts ofNierembergia repens by inducing single shoots on the microcolonies (1997)
    Springer
    Plant cell reports 16 (1997), S. 545-549 
    Details
    ISSN: 1432-203X
    Keywords: 6-benzylaminopurine ; Leaf protoplast ; 1-Naphthaleneacetic acid ; Nierembergia repens ; Plant regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A simple and efficient protocol for plant regeneration from mesophyll protoplasts ofNierembergia repens is described. The protoplasts divided in modified half-strength MS (/12 MS) medium containing benzylaminopurine (BA) and a-naphthaleneacetic acid (NAA) and formed visible colonies after 2 weeks which produced single adventitious shoots 4 weeks later. Plating efficiency (11.2%), percent colony formation (0.84%), and the number of shoot-forming colonies (368/dish) were highest in /12 MS containing 0.1 mg/l BA and 0.05 mg/l NAA. However, the percentage of colonies with shoot formation was highest (31.8%) in /12 containing 0.05 mg/l BA and 0.01 mg/l NAA. Almost all of the remaining colonies (97.5%) also regenerated shoots upon transfer onto MS medium containing 0.05 mg/l BA. The shoots with 2–3 leaves readily rooted 3–5 days after insertion in /12MS lacking plant growth regulators. Regenerated plantlets were easily established in soil 50 days after protoplast isolation. All the regenerants were normal and possessed diploid chromosome numbers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01142321
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  • 3
    Electronic Resource
    Electronic Resource
    Agrobacterium-mediated genetic transformation of a phalaenopsis orchid (2000)
    Springer
    Plant cell reports 19 (2000), S. 435-442 
    Details
    ISSN: 1432-203X
    Keywords: Key words Acetosyringone ; Agrobacterium tumefaciens ; Genetic transformation ; Plant regeneration ; Orchid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Genetically transformed plants of a phalaenopsis orchid [Doritaenopsis Coral Fantasy×Phalaenopsis (Baby Hat×Ann Jessica)] were regenerated after cocultivation of cell clumps with Agrobacterium tumefaciens strains LBA4404 (pTOK233) and EHA101 (pIG121Hm) that harbored genes for β-glucuronidase (GUS) and hygromycin resistance. The efficiency of transformation was markedly increased by 10 h cocultivation of cell clumps with A. tumefaciens that had been induced with 200 μm acetosyringone, and by inclusion of 500 μm acetosyringone in the cocultivation medium. Hygromycin-resistant cell clusters (0.5–3 mm in diameter) were selected from the infected cell clumps after 4–6 weeks of culture on agar (8 g/l)-solidified new Dogashima medium (NDM) containing 20 g/l sucrose, 0.1 mg/l naphthaleneacetic acid, 1.0 mg/l benzyladenine (BA), 50 mg/l hygromycin and 300 mg/l cefotaxime. The cell clusters proliferated 4 weeks after transfer onto the same medium. To induce callus greening, the carbon source was changed from sucrose to maltose. The green calli obtained produced protocorm-like bodies (PLBs) after 4 weeks of culture on phytohormone-free NDM medium. Regeneration of transgenic plantlets was enhanced by incubating PLBs on NDM medium supplemented with 0.1 mg/l abscisic acid, followed by partial desiccation for 10–30 min. Successful transformation was confirmed by histochemical GUS assay, PCR analysis and Southern hybridization of transformants. With this transformation system, more than 100 hygromycin-resistant phalaenopsis plantlets were produced about 7 months following infection of the cell aggregates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050752
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  • 4
    Electronic Resource
    Electronic Resource
    Plant regeneration from hairy roots induced by infection with Agrobacterium rhizogenes in Crotalaria juncea L. (2000)
    Springer
    Plant cell reports 19 (2000), S. 563-568 
    Details
    ISSN: 1432-203X
    Keywords: Key words Agrobacterium rhizogenes ; Crotalaria juncea ; Plant regeneration ; Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Hairy roots were induced from leaf segments of Crotalaria juncea, which is used as a green manure crop antagonistic to nematodes, by infection with a mikimopine type wild strain of Agrobacterium rhizogenes A13 (MAFF02-10266). These roots exhibited vigorous growth and abundant lateral branching on half-strength Murashige and Skoog (1/2MS) medium without phytohormones. The adventitious shoots were induced from 30% of root segments 3 months after transfer onto medium containing 3 mg/l benzyl adenine. These shoots produced roots 1 month after transfer onto 1.2% agar-solidified 1/2MS medium without phytohormones. Regenerated plants were successfully grown under greenhouse conditions. The transgenic nature of the regenerated plants was confirmed by Southern-blot analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050774
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  • 5
    Electronic Resource
    Electronic Resource
    Shoot regeneration from spinach hypocotyl segments by short term treatment with 5,6-dichloro-indole-3-acetic acid (1992)
    Springer
    Plant cell reports 11 (1992), S. 58-61 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Factors affecting shoot regeneration from hypocotyl segments of spinach (Spinacia oleracea L.) were investigated. When expiants were cultured on medium containing 10 mg/l IAA for 7 weeks, 3 out of 9 cultivars showed relatively high shoot regeneration response (15 – 35%). The other PGRs tested had no effect on shoot regeneration. However, the transfer of explants from auxin-containing medium to auxin-free medium 20 d after culture induced shoot formation from expiants cultured on media containing each of the auxin sources tested individually. By applying this short term auxin treatment, more than 80% shoot regeneration was obtained on medium containing 5–20 mg/l 5,6-Cl2-IAA, compared to less than 30% with 10–20 mg/l IAA treatment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00235253
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  • 6
    Electronic Resource
    Electronic Resource
    Bialaphos stimulates shoot regeneration from hairy roots of snapdragon (Antirrhinum majus L.) transformed by Agrobacterium rhizogenes (1998)
    Springer
    Plant cell reports 17 (1998), S. 256-261 
    Details
    ISSN: 1432-203X
    Keywords: Key words Adventitious shoot regeneration ; Agrobacterium rhizogenes ; Antirrhinum majus L. ; Bialaphos ; Hairy roots
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hairy roots of snapdragon (Antirrhinum ma-jus L.: Scrophulariaceae) induced by a wild-type strain of Agrobacterium rhizogenes were cultured on media containing various concentrations of a phosphinothricin-based herbicide, bialaphos, or plant growth regulators (PGRs). Adventitious shoot regeneration from hairy roots was observed with a low frequency (10%) on half-strength Murashige and Skoog medium. Addition of α-naphthalene-acetic acid in combination with 6-benzylaminopurine, thidiazuron, or zeatin to the medium had no effect on shoot regeneration from hairy roots. Although bialaphos at 0.9 mg l–1 or more was toxic to hairy roots, it significantly increased the shoot regeneration frequency up to 56% at 0.5 mg l–1. In contrast, non-transformed roots and leaves regenerated no shoots on media with or without bialaphos. Regenerated shoots detached from host roots readily developed roots on gellan-gum-solidified medium. Regenerated plants were successfully transferred to the greenhouse, but did not produce seed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050388
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  • 7
    Electronic Resource
    Electronic Resource
    Cryopreservation of Doritaenopsis suspension culture by vitrification (2000)
    Springer
    Plant cell reports 19 (2000), S. 1160-1164 
    Details
    ISSN: 1432-203X
    Keywords: KeywordsDoritaenopsis ; Cryopreservation ; Abscisic acid ; Protocorm-like body ; Abbreviations ABA: Abscisic acid ; BA6: Benzylaminopurine ; DMSO: Dimethylsulphoxide ; NAA: Naphthaleneacetic acid ; ND: New¶Dogashima ; PLB: Protocorm-like body ; TTC: 2,3,5-Triphenyltetrazolium chloride
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Cells of a suspension culture of Doritaenopsis cv. New Toyohashi were placed in a mixture of 2 M glycerol and 0.4 M sucrose for 15 min at room temperature and then dehydrated with a vitrification solution (PVS2) for 1–3 h on ice and plunged into liquid nitrogen. The highest viability (64% by 2,3,5-triphenyltetrazolium chloride stainability) was obtained when the cells were precultured in liquid New Dogashima medium with 0.1 M sucrose and 1.0 mg/l abscisic acid for 1 week at 25  °C in the light. Dehydration by PVS2 was important for the cryopreservation of Doritaenopsis cells. Protocorm-like bodies were induced from cryopreserved cells without morphological variations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990000255
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  • 8
    Electronic Resource
    Electronic Resource
    Differences in ploidy levels of inter-specific hybrids obtained by reciprocal crosses between Primula sieboldii and P. kisoana (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 690-696 
    Details
    ISSN: 1432-2242
    Keywords: Key words Inter-specific hybrid ; Primulasieboldii ; Primulakisoana ; Triploid hybrid ; Unreduced female gamete
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Differences in ploidy level were found in inter-specific hybrids obtained by reciprocal crosses between Primula sieboldii and P. kisoana. When P. sieboldii was used as the maternal parent, the inter-specific hybrids were triploids; when P. kisoana was the maternal parent, the inter-specific hybrids were diploids. The possibility of diploid female gamete formation in P. sieboldii is discussed as a causal factor in the production of triploids occasionally found in crosses between diploids of this species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051532
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  • 9
    Electronic Resource
    Electronic Resource
    Construction of synteny groups of Brassica alboglabra by RAPD markers and detection of chromosome aberrations and distorted transmission under the genetic background of B. campestris (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 538-546 
    Details
    ISSN: 1432-2242
    Keywords: Key words Brassica campestris ; Brassica alboglabra ; Chromosome addition ; RAPD markers ; Intergenomic recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Interspecific hybrids were produced by crosses between the inbred lines of B. campestris and B. alboglabra, and were backcrossed twice to B. campestris. Genetical constitutions of the BC2 plants were analyzed by RAPD (random amplified polymorphic DNA), flow cytometry and cytological observations. By using 140 arbitrary primers, a total of 137 polymorphic bands were obtained and 125 were found to be specific to B. alboglabra. Based on the presence and absence of the specific RAPD markers of B. alboglabra, 13 synteny groups were constructed. The number of markers in each synteny group was found to be different and varied from 2 to 28. This reflects the difference in the degree of genetic variability among the B. alboglabra chromosomes from those of B. campestris. Losses or gains of RAPD markers were observed frequently in most of the synteny groups, which indicated the occurrence of chromosome translocations and/or deletions in the chromosomes of B. alboglabra. In a population of 40 BC2 plants, chromosome transmission rates were analyzed by using the RAPD markers in each synteny group. Most of the chromosomes of the synteny groups were transmitted with rates of 0.37–0.68. An extremely high transmission rate, 0.98, was however observed in one of the synteny groups. Inheritance data of the synteny groups revealed relationships among themselves. The plants lacking the RAPD markers of two synteny groups tended to lose others belonging to the rest of the synteny groups, indicating the effects of these groups on the transmission of B. alboglabra chromosomes to the B. campestris background.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051513
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  • 10
    Electronic Resource
    Electronic Resource
    Transplantation of isolated nuclei into plant protoplasts (1986)
    Springer
    Planta 168 (1986), S. 29-35 
    Details
    ISSN: 1432-2048
    Keywords: Auxotrophy ; Datura ; DNA transfer ; Nuclear transplantation ; Protoplast ; Prototrophy ; Vicia (nuclear transplantation)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The uptake of isolated nuclei from Vicia hajastana Grossh. cells into protoplasts of an auxotrophic cell line of Datura innoxia P. Mill. was induced under the influence of polyethylene glycol and Ca2+ at pH 6.8. The frequency of nuclear uptake varied from 0.8 to 2.3% and that of the recovery of prototrophic clones from 10-5 to 6·10-4. The prototrophic nuclear fusion products following nuclear uptake could be rescued by initial culture of the protoplasts in non-selective conditions and by the subsequent use of feeder cell layers to support the growth of surviving colonies on a selective medium. The presence of Vicia genomic DNA in some prototrophic clones was confirmed by dot-blot hybridization using Datura and Vicia DNA probes. In certain transformed clones, the recovery of prototrophy was accompanied by the restoration of morphogenetic potential. Welldeveloped shoots typical of wild-type Datura could be regenerated employing an appropriate regeneration medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00407005
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