ISSN:
1617-4623
Keywords:
Streptomyces clavuligerus
;
Linear plasmid
;
Terminal repeats
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A linear DNA plasmid (pSCL) has been isolated from Streptomyces clavuligerus by a method employing high concentrations of protease. Rate-zonal sedimentation on sucrose gradients was used to purify the plasmid. The plasmid is 12 kb in length and appears to be linked to protein at its 5′ termini. A restriction endonuclease map of the plasmid for ten enzymes has been determined. Evidence for terminally repeated sequences is provided by cross-hybridization analysis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00322461
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