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Isolation and characterization of a linear DNA plasmid from Streptomyces clavuligerus

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Summary

A linear DNA plasmid (pSCL) has been isolated from Streptomyces clavuligerus by a method employing high concentrations of protease. Rate-zonal sedimentation on sucrose gradients was used to purify the plasmid. The plasmid is 12 kb in length and appears to be linked to protein at its 5′ termini. A restriction endonuclease map of the plasmid for ten enzymes has been determined. Evidence for terminally repeated sequences is provided by cross-hybridization analysis.

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Author notes

  1. Cheryl L. Keen

    Present address: Department of Microbiology, University of British Columbia, V6T 1W5, Vancouver, BC, Canada

Authors and Affiliations

  1. Department of Microbiology, University of Alberta, T6G 2E9, Edmonton, Alta, Canada

    Cheryl L. Keen & Kenneth L. Roy

  2. Department of Microbiology, Tel Aviv University, Tel Aviv, Israel

    Simona Mendelovitz, Gerald Cohen & Yair Aharonowitz

Authors
  1. Cheryl L. Keen
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  2. Simona Mendelovitz
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  3. Gerald Cohen
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  4. Yair Aharonowitz
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  5. Kenneth L. Roy
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Communicated by W. Goebel

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Keen, C.L., Mendelovitz, S., Cohen, G. et al. Isolation and characterization of a linear DNA plasmid from Streptomyces clavuligerus . Molec. Gen. Genet. 212, 172–176 (1988). https://doi.org/10.1007/BF00322461

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  • DOI: https://doi.org/10.1007/BF00322461

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