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  • 1
    Electronic Resource
    Electronic Resource
    Isolation and molecular characterisation of the gene encoding the cytoplasmic ribosomal protein S28 in Prunus persica [L.] Batsch (2000)
    Springer
    Molecular genetics and genomics 263 (2000), S. 201-212 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsPrunus persica ; Ribosomal proteins ; Gene expression ; Gene regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract RT-PCR was performed on peach (Prunus persica [L.] Batsch) RNA to isolate cDNAs corresponding to transcripts which are differentially expressed in leaves borne on basal and apical shoots. A gene was identified which was more highly expressed in the leaves of basal shoots, and codes for the cytoplasmic protein S28 present in the small ribosomal subunit. The 5′ leader regions of RPS28 mRNAs were found to harbour 8–11 pyrimidine tracts, which suggested similarities to regulatory stretches that control the translation of mRNAs for ribosomal proteins in animals. The peach S28 is encoded by two intron-containing genes, which are both transcribed in mitotically active tissues such as developing leaves and roots. In situ hybridisation to shoot vegetative apices and the measurement of nucleus/nucleolus ratios indicated that RPS28 expression was confined to areas undergoing active cell division. The mature RPS28 mRNA was detected as a single species in actively dividing tissues such as apical tips, developing leaves, vegetative buds, stamens, developing fruits and roots. In contrast, accumulation of a precursor RNA, in the presence of the mature product, was found in fully expanded leaves and subtending stems, while only the precursor species was detected in several late-stage tissues. This phenomenon suggested that expression of the mature RNA is controlled at the level of splicing and turnover of the precursor RNA. This is similar to the mode of regulation of ribosomal protein genes in animals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380051161
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  • 2
    Electronic Resource
    Electronic Resource
    Agrobacterium-mediated transformation of Artemisia absinthium L. (wormwood) and production of secondary metabolites (1997)
    Springer
    Plant cell reports 16 (1997), S. 725-730 
    Details
    ISSN: 1432-203X
    Keywords: Key words Wormwood ; Hairy roots ; Genetic transformation ; Essential oil ; Secondary metabolites
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hairy roots were obtained after infection of Artemisia absinthium shoots with Agrobacterium rhizogenes strains 1855 and LBA 9402. The susceptibility to hairy root transformation varied between plant genotypes and bacterial strains. Hairy roots showed macroscopic differences from control root cultures. Southern blot hybridization confirmed the integration of T-DNA from both p1855 and pBin19, while polymerase chain reaction analysis indicated the presence of the neomycin phosphotransferase gene in the hairy root genome. Subcultured transformed root lines grew well in selective B5 agar-solidified medium containing kanamycin or rifampicin and without hormones. Shake-flask experiments with fast-growing root lines showed that 40 g l–1 was the best sucrose concentration for biomass production, yielding a 463-fold increase in dry weight after 28 days of culture. Great differences were found in the profiles of the essential oils isolated from normal and hairy roots. Gas chromatography/mass spectrometry analysis showed the oil produced by transformed cultures to be a mixture of 50 compounds with only one major component representing 37% of the oil content.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050310
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  • 3
    Electronic Resource
    Electronic Resource
    Chlorsulfuron resistance in Daucus carota cell lines and plants:Involvement of gene amplification (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 520-524 
    Details
    ISSN: 1432-2242
    Keywords: Daucus carota L. ; Cell selection ; Chlorsulfuron resistance ; Acetohydroxyacid synthase ; Gene amplification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Daucus carota L. cell lines stably resistant to the herbicide chlorsulfuron (CS) have been isolated according to a stepwise selection. Studies carried out during different selection steps show that the specific activity of the target enzyme acetohydroxyacid synthase (AHAS) increases along with CS resistance. Southern hybridization analysis performed with aBrassica napus AHAS probe in a CS highly-resistant cell line reveals the presence of a greatly amplifiedEcoRI fragment of genomic DNA. This indicates that AHAS overproduction induced by stepwise selection is due to gene amplification. Regenerants from some resistant cell lines maintained the CS-resistant trait at the whole plant level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01240912
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  • 4
    Electronic Resource
    Electronic Resource
    Hairy root transformation in alfalfa (Medicago sativa L.) (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 523-530 
    Details
    ISSN: 1432-2242
    Keywords: Hairy root ; Medicago sativa L. ; Phenotypic variation ; Agrobacterium rhizogenes ; Genetic transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The widely cultivated forage legume alfalfa (Medicago sativa L.) was transformed with the agropine type Agrobacterium rhizogenes NCPPB 1855. Sterile root and callus cultures were derived from tumorous hairy roots which were easily obtained independent of the plant variety or genotype. Plant regeneration, via somatic embryogenesis, was achieved only when a selected alfalfa line, characterized by high regenerative capability, was utilized. Genetic transformation was confirmed by the presence of agropine and T-DNA. Phenotypic alterations, mainly affecting the root system, were observed in transformed plants. The possibility that T-DNA-induced variations could be useful in the improvement of M. sativa is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00289189
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  • 5
    Electronic Resource
    Electronic Resource
    Time-related behaviour of phenotypic variation in Lotus corniculatus regenerants under field conditions (1985)
    Springer
    Euphytica 34 (1985), S. 619-623 
    Details
    ISSN: 1573-5060
    Keywords: Lotus corniculatus ; birdsfoot trefoil ; tissue culture ; somaclonal variation ; alteration stabiliy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Plants of Lotus corniculatus regenerated from single genotype-derived calli showed for several agronomic and morphological traits a variation similar to that present in the original population. Comparisons of the results obtained under field conditions during the 2nd and 3rd year from plant regeneration show: a) the maintenance of significant variance within callus-derived population for several traits examined; b) the total disappearance of ‘minus variants’ indicating a time-related decrease of depressive in vitro effects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00035397
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  • 6
    Electronic Resource
    Electronic Resource
    Synthesis of extracellular proteins in embryogenic and non-embryogenic cell cultures of alfalfa (1996)
    Springer
    Plant cell, tissue and organ culture 44 (1996), S. 257-260 
    Details
    ISSN: 1573-5044
    Keywords: 2,4-dichlorophenoxyacetic acid ; Medicago sativa ; somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Extracellular proteins, released into the culture medium from alfalfa cells grown in embryogenic and non-embryogenic conditions, were 35S-methionine labelled at different days of culture. SDS-PAGE analysis showed significant differences between the patterns of extracellular proteins secreted into the medium devoid of 2,4-d, in which cells formed somatic embryos, or in presence of 2,4-d, in which undifferentiated cell proliferation took place. Some proteins, evident in 2,4-d-supplied cultures, disappeared when cells were subcultured in the embryogenic conditions. Western analysis with antibodies against the carrot extracellular proteins EP1 and EP2 showed the presence of homologous alfalfa proteins. In 2,4-d depleted alfalfa cells, an EP1-like protein disappeared and another one was reduced, while the presence of the EP2-like protein was, in the same conditions, strongly enhanced.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00048533
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  • 7
    Electronic Resource
    Electronic Resource
    Different promoter regions control level and tissue specificity of expression of Agrobacterium rhizogenes rolB gene in plants (1991)
    Springer
    Plant molecular biology 16 (1991), S. 427-436 
    Details
    ISSN: 1573-5028
    Keywords: Agrobacterium rhizogenes ; rolB promoter ; GUS ; tissue specificity ; transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Expression of the rolB gene of A. rhizogenes T-DNA triggers root differentiation in transformed plant cells. In order to study the regulation of this morphogenetic gene, the GUS reporter gene was placed under the control of several deleted fragments of the rolB 5′ non-coding region: carrot disc transformations and the analysis of transgenic tobacco plants containing these constructions identified the presence of distinct regulatory domains in the rolB promoter. Two regions (located from positions −623 to −471 and from −471 to −341, from the translation start codon) control the level but not the tissue specificity of rolB expression: progressive deletions of the rolB promoter starting from position −1185 to −341, although at different levels, maintained the same pattern of GUS expression — maximal in root meristems and less pronounced in the vascular tissue of aerial organs. Further deletion of 35 bp, from −341 to −306, drastically affected tissue specificity: GUS activity was still clearly detectable in the vascular tissue of the aerial organs while expression in the root meristem was totally suppressed. Analysis of transgenic embryos and seedlings confirmed that distinct promoter domains are responsible for meristematic (root) and differentiated (vascular) expression of rolB. Finally, we present data concerning the effects of plant hormones on the expression of rolB-GUS constructions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023993
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  • 8
    Electronic Resource
    Electronic Resource
    Agrobacterium rhizogenes T-DNA genes capable of inducing hairy root phenotype (1987)
    Springer
    Molecular genetics and genomics 209 (1987), S. 475-480 
    Details
    ISSN: 1617-4623
    Keywords: Agrobacterium ; Hairy root ; T-DNA ; Transgenic tobacco
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Segments of the TL-DNA of the agropine type Ri plasmid pRi 1855 encompassing single and groups of open-reading frames were cloned in the Ti plasmid-derived binary vector system Bin 19. Leaf disc infections on Nicotiana tabacum led to transformed plants, some of which showed typical hairy root phenotypes, such as the wrinkled leaf morphology, excessive and partially non geotropic root systems and the ability of leaf explants to differentiate roots in a hormone-free culture medium. Particularly interestingly, most of these traits were shown by plants transformed with a TL-DNA segment encompassing the single ORF 11, corresponding to the rolB locus. Hairy root can be induced by this latter T-DNA segment on wounded stems of tobacco plants; hairy root induction on carrot discs requires, on the contrary, a more complex complement of TL-DNA genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331152
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