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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Naturwissenschaften 74 (1987), S. 445-446 
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 1987-09-01
    Print ISSN: 0028-1042
    Electronic ISSN: 1432-1904
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Published by Springer
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Development genes and evolution 180 (1976), S. 31-46 
    ISSN: 1432-041X
    Keywords: Cell lineage ; Determination ; Fate Mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cell lineage relationships observable inDrosophila gynandromorphs have been used to locate the primordia of the individual thoracic disc relative to each other in the embryo. Three observations indicate that the borders of the individual disc primordia lie very close to each other, separated by few if any non-disc cells.First, the frequency of mosaicism within each disc indicates large primordia, of similar magnitude to the distances between the centres of adjacent primordia.Second, very few XX/XO-borders cut between adjacent discs without also cutting into one or the other disc.Third, sturt distances between points on adjacent discs are often much smaller than distances measured within individual discs. The proximity of disc primordia suggests that the individual discs might share common precursor cells in the early embryo.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Development genes and evolution 184 (1978), S. 75-82 
    ISSN: 1432-041X
    Keywords: Egg shape ; Pole cell transplantation ; Sterility ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Females homozygous for a newly isolated mutation induced by ethyl methane sulphonate,fs(1)K10, lay abnormally shaped eggs in which the dorsal appendages of the chorion are enlarged and fused ventrally. The eggs are usually not fertilized and development is never normal beyond the blastoderm stage. The mutant was mapped to the tip of the X-chromosome with a meiotic position of 1–0.5 and a cytological location between 2B17 and 3A3. Using germ line mosaics constructed by transplantation of pole cells, it was shown that the abnormal morphology and the sterility are obtained only when the germ line is homozygous for the mutant.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Development genes and evolution 187 (1979), S. 151-165 
    ISSN: 1432-041X
    Keywords: Oogenesis ; Embryogenesis ; Two-dimensional gels ; Protein synthesis ; Drosophila melanogaster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Protein synthesis in egg follicles and blastoderm embryos ofDrosophila melanogaster has been studied by means of two-dimensional gel electrophoresis. Up to 400 polypeptide spots have been resolved on autoradiographs. Stage 10 follicles (for stages see King, 1970) were labelled in vitro for 10 to 60 min with35S-methionine and cut with tungsten needles into an anterior fragment containing the nurse cells and a posterior fragment containing the oocyte and follicle cells. The nurse cells were found to synthesize a complex pattern of proteins. At least two proteins were detected only in nurse cells but not in the oocyte even after a one hour labelling period. Nurse cells isolated from stages 9, 10 and 12 follicles were shown to synthesize stage specific patterns of proteins. Several proteins are synthesized in posterior fragments of stage 10 follicles but not in anterior fragments. These proteins are only found in follicle cells. No oocyte specific proteins have been detected. Striking differences between the protein patterns of anterior and posterior fragments persist until the nurse cells degenerate. In mature stage 14 follicles, labelled in vivo, no significant differences in the protein patterns of isolated anterior and posterior fragments could be detected; this may be due to technical limitations. At the blastoderm stage localized synthesis of specific proteins becomes detectable again. When blastoderm embryos, labelled in vivo, are cut with tungsten needles and the cells are isolated from anterior and posterior halves, differences become apparent. The pole cells located at the posterior pole are highly active in protein synthesis and contribute several specific proteins which are found exclusively in the posterior region of the embryo. In this study synthesis of specific proteins could only be demonstrated at those developmental stages which are characterized by the presence of different cell types within the egg chamber, while no differences were detected when stage 14 follicles were cut and anterior and posterior fragments analyzed separately. The differences in the pattern of protein synthesis by pole cells and blastoderm cells indicate that even the earliest stages of determination are reflected by marked changes at the biochemical level.
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  • 6
    ISSN: 1432-041X
    Keywords: Apis mellifera ; Homeobox genes ; Dfd ; In situ hybridization ; Blastoderm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have isolated and characterized a homeoboxcontaining gene from the honeybee Apis mellifera. Its homeobox region shows a high degree of sequence similarity to the homeobox of the Drosophila gene Deformed (Dfd). At the DNA level 82% of the basepairs are the same, whereas the putative amino acid sequences are identical between the bee and the fruitfly genes. Similarity is also present 5′ and 3′ to the homeobox. Using this isolate as a probe we have performed in situ hybridization on sections from blastoderm-stage embryos of the honeybee Apis mellifera. In early blastoderm stages we found a rather irregular pattern of labelled nuclei. In middle stages we found silver grains over each nucleus and also over the cytoplasm in a belt of blastoderm cells in the prospective gnathal region. These results indicate that the Deformed genes from honeybee and fruitfly are homologous both with respect to their DNA sequence and their spatial and temporal pattern of expression during embryogenesis.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-041X
    Keywords: Drosophila ; Homeotic gene regulation ; Antennapedia ; Development ; β-galactosidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In order to study the regulation of spatial and temporal expression of the homeotic gene Antennapedia (Antp) in Drosophila melanogaster, we have constructed fusion genes which contain Antp sequences linked to the reporter gene lac Z of Escherichia coli. In one case of P-element transformation, a fusion gene construct integrated into the endogenous Antp gene close to one of the two promoters (P1). The spatial expression from the reporter gene in this transformant line, as analysed by the detection of β-galactosidase activity, was found to exactly mimic the normal expression from the P1 promoter of the Antp gene. We have used this unique transformant as a tool for studying the expression of the P1 promoter in embryonic, larval and adult development. Parallel lines transformed with the same fusion gene construct did not confer a correct P1 pattern of expression. The position in the genome was, therefore, crucial for the expression pattern of the reporter gene. Experiments aiming at the detection of autoregulatory control of Antp gene expression were designed. The results did not, however, support models of positive or negative autoregulation of P1 expression by Amp protein.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 54 (1998), S. 272-276 
    ISSN: 1420-9071
    Keywords: Key words. Natriuretic peptide; kinetin; abscisic acid; cGMP; stomata; guard cells.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Immunological evidence suggests that plants contain natriuretic peptides (NPs) and furthermore (3- [125I]iodotyrosol28) rat atrial NP (rANP) binds specifically to plant membranes. rANP and immunoaffinity-purified plant NP analogues also promote concentration-dependent stomatal opening. Here we report that kinetin, a synthetic cytokinin, and rANP induce stomatal opening in Tradescantia albiflora and that the effect of rANP is critically dependent on the secondary structure of the peptide hormone. The native circular molecule is active, whereas the linearized molecule shows no biological activity. Furthermore, kinetin- and rANP-induced stomatal opening is reversibly inhibited by two in hibitors of guanylate cyclase, LY 83583 and methylene blue. Stomatal opening is also induced in a concentration-dependent manner by the cell-permeant cyclic guanosine-3′,5′-monophosphate (cGMP) analogue 8-Br-cGMP, and this effect is prevented by the stomatal closure promoting plant hormone abscisic acid (ABA). We conclude that in guard cells kinetin and rANP pathways operate via guanylate cyclase upregulation, and we propose that ABA-induced closure is not cGMP-dependent.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Development genes and evolution 201 (1992), S. 235-242 
    ISSN: 1432-041X
    Keywords: Honeybee ; Embryogenesis ; Homeotic genes ; Deformed ; Antibody staining
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have raised antiserum against part of the Deformed (Dfd) protein of the honeybee and describe here the expression pattern of the Dfd protein during honeybee embryogenesis. Dfd protein is first stained in the prospective gnathal region of the cellular blastoderm. This circumferential band corresponds to the distribution of Dfd mRNA described earlier, and to the blastodermal Dfd expression pattern in Drosophila. Using an antibody against the engrailed (en) protein of Drosophila, we found that at the beginning of gastrulation Dfd expression in the honeybee, as in Drosophila, is restricted to the future intercalary, mandibular and maxillary segments. During gastrulation, the mesodermal nuclei loose the Dfd label gradually from anterior to posterior, and in the ectoderm the most posterior ventral cells loose Dfd while retaining en staining; thus, in contrast to what has been described for Drosophila, the posterior Dfd expression border seems to move forward ventrally to the parasegmental boundary within the maxillary segment. In the late germ band, the lateral tips of the Dfd-expressing band are connected across the dorsal side by a row of amnion cells with strongly staining large nuclei. After dorsal closure, a narrow stripe of Dfd-staining dorsal cells behind the neck region may indicate that the maxillary segment contributes to the dorsal body wall posterior to the head capsule. Thus, apart from some minor deviations, the Dfd expression pattern in the honeybee strongly resembles that in Drosophila prior to head involution. This is compatible with the assumption that head involution (which is a special adaption in higher dipterans) ensues after a rather conserved course of early head development in which Dfd appears to play a basic role.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    The journal of supercomputing 11 (1997), S. 355-369 
    ISSN: 1573-0484
    Keywords: message passing ; computer resource management ; communication protocols ; PVM ; MPI
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract Parallel process communication and system resource management have been seen as two separate entities in parallel and distributed systems. This causes difficulties in the dynamic mapping of newly spawned processes, because the application has little or no information on the availability, the connectivity and the current work-load of the target system. As a consequence, process mappings are often sub-optimal, overloading resources on one system while other processors are idling. We present a software system named "PLUS" that provides • interprocess communication between different message passing models such as MPI, PVM and PARIX, and • access to resource management systems for optimal process mapping and task migration. PLUS is a light-weight, extensible and efficient communication interface. With only four commands, PLUS is almost transparent ot the application code. Our current implementation supports inter-process communication between PVM, MPI and PARIX, but it can be easily extended to other vendor-specific message passing libraries. As PLUS has been designed for wide area networks, much effort has been spent on portability and on optimizing the communication speed across internet and also intranet links.
    Type of Medium: Electronic Resource
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