ISSN:
1617-4623
Keywords:
D. melanogaster
;
Heat shock
;
RNA splicing
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Heat-shock locus 93D of Drosophila melanogaster consists of an internally repetitive and a neighbouring unique region. The unique part contains a promoter that is already active in non-heat-shocked cells but shows fivefold enhanced transcription after stress induction. In third instar larvae, a series of 93D-derived RNA products appear, which might be the result of incomplete processing events. The major RNA species (1.2 kb) is the splicing product of a poly(A)+-containing primary transcript of 1.9 kb. Furthermore, a transcript of high molecular weight is observed, which in addition contains the 3′-flanking 93D-specific ‘TaqI repeat’ sequences. This readthrough transcript is found in the poly(A)+ as well as in the poly(A)- RNA fraction. After severe heat shock, the already limited processing efficiency of 93D RNA is further inhibited. Production of the readthrough transcript, on the other hand, is reduced. DNA sequence analyses of genomic and cDNA sequences reveal that this 93D heat-shock gene contains only a very limited protein-coding capacity. A coding function for the mature 93D heat-shock RNA is therefore questionable.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00425519
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