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RFLP mapping of manganese efficiency in barley (2000)

Pallotta, M. A. ; Graham, R. D. ; Langridge, P. ; [et al.]

Springer

Theoretical and applied genetics 101 (2000), S. 1100-1108

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ISSN: 1432-2242

Keywords: Key words Barley ; Manganese efficiency ; RFLP mapping ; Marker-assisted Selection ; Plant nutrition ; 4HS

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract In many cropping regions of the world, yield is limited by the availability of micronutrients, and micronutrient-efficient cultivars provide a yield advantage. Traditional methods of testing cultivars for micronutrient efficiency are time-consuming and laborious. Molecular markers linked to loci controlling micronutrient efficiency will allow more rapid and efficient selection and introgression of these traits than is currently possible. Using a pot-based bioassay and bulked segregant analysis of an F2 population, we have identified several RFLPs (grouped distally on chromosome 4HS) linked to a locus for manganese efficiency in barley. This manganese efficiency locus has been designated Mel1. Pot bioassay analysis of intercrosses suggests that three useful sources of manganese efficiency are likely to be allelic at the Mel1 locus. Field evaluation of marker selected F4 progeny supports the major role of Mel1 in the genetic control of manganese efficiency. Adoption of marker assisted selection for this trait in the Southern Australian barley breeding program has occurred. This has been facilitated by the demonstration that the Mel1 allele of Amagi Nijo can be distinguished from 95 other locally useful varieties and breeder’s lines on the basis of RFLPs identified by just two molecular markers.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s001220051585

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RFLP mapping of the Ha 2 cereal cyst nematode resistance gene in barley (1997)

Kretschmer, J. M. ; Chalmers, K. J. ; Manning, S. ; [et al.]

Springer

Theoretical and applied genetics 94 (1997), S. 1060-1064

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ISSN: 1432-2242

Keywords: Key wordsCCN ; RFLP ; Hordeum vulgare ; Heterodera avenae ; Genetic mapping

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The cereal cyst nematode (CCN), Heterodera avenae Woll., is an economically damaging pest of barley in many of the world’s cereal-growing areas. The development of CCN-resistant cultivars may be accelerated through the use of molecular markers. A number of resistance genes against the pest are well known; one of them, the single dominant Ha 2 resistance gene, has been shown to be effective against the Australian pathotype and maps to chromosome 2 of barley. Segregation analysis identified two restriction fragment length polymorphism (RFLP) markers flanking the resistance gene in two doubled-haploid populations of barley. AWBMA 21 and MWG 694 mapped 4.1 and 6.1 cM respectively from the Ha 2 locus in the Chebec×Harrington cross and 4.0 and 9.2 cM respectively in the Clipper×Sahara cross. Analysis of a further seven sources of CCN resistance in the form of near-isogenic lines (NILs) indicates that all available sources of resistance to the Australian pathotype of CCN in barley represent the Ha 2 locus.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s001220050515

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