ALBERT

Notes: Abstract. Genetic engineering, coupled with spectro scopic analyses, has enabled the metal binding proper ties of the α and β subunits of mouse metallothionein 1 (MT) to be characterized. A heterologous expression system in E.coli has led to high yields of their pure zinc-complexed forms. The cadmium(II) binding properties of recombinant Zn4-αMT and Zn3-βMT have been studied by electronic absorption and circular dichroism. The former binds Cd(II) identically to α fragments obtained from mammalian organs, showing that the recombinant polypeptide behaves like the na tive protein. Titration of Zn3-βMT with CdCl2 results in the formation of Cd3-βMT. The addition of excess Cd(II) leads to Cd4-βMT which, with the extra loading of Cd(II), unravels to give rise isodichroically to Cd9-βMT. The effect of cadmium-displaced Zn(II) ions and excess Cd(II) above the full metal occupancy of three has been studied using Chelex-100. The Cd3-βMT species is stable in the presence of this strong metal-chelating agent.

Notes: Abstract Three-hourly average values of the Dst, AE and ap geomagnetic activity indices have been studied for 1 year’s duration near the solar minimum (1974) and also at the solar maximum (1979). In 1979 seven intense geomagnetic storms (Dst 〈 –100 nT) occurred, whereas in 1974 only three were reported. This study reveals: (1) the yearly average of AE is greater in 1974 than in 1979, whereas the inverse seems to be true for the yearly average of Dst, when a higher number of intense storms is present. These averages indicate the kind of activity occurring on the sun as shown in earlier work. (2) The seasonal variation of Dst is higher than that of ap and is almost negligible in AE. (3) The correlation coefficient of ap × AE is in general the highest, as the magnetometers that monitor both indices are close, and is surpassed only by the ap × Dst correlation during geomagnetic storms, when the influence of the ring current is dominant. The correlation of ap × Dst also shows a seasonal variability. (4) For the first time a study of correlation between ap and a linear combination of AE and Dst has also been made. We found higher correlation coefficients in this case as compared to those between ap × Dst and ap ×xs AE.

Notes: Abstract We present some results of the analytical integration of the energy rate balance equation, assuming that the input energy rate is proportional to the azimuthal interplanetary electric field, Ey, and can be described by simple rectangular or triangular functions, as approximations to the frequently observed shapes of Ey, especially during the passage of magnetic clouds. The input function is also parametrized by a reconnection-transfer efficiency factor α (which is assumed to vary between 0.1 and 1). Our aim is to solve the balance equation and derive values for the decay parameter τ compatible with the observed Dst peak values. To facilitate the analytical integration we assume a constant value for τ through the main phase of the storm. The model is tested for two isolated and well-monitored intense storms. For these storms the analytical results are compared to those obtained by the numerical integration of the balance equation, based on the interplanetary data collected by the ISEE-3 satellite, with the τ values parametrized close to those obtained by the analytical study. From the best fit between this numerical integration and the observed Dst the most appropriate values of τ are then determined. Although we specifically focus on the main phase of the storms, this numerical integration has been also extended to the recovery phase by an independent adjust. The results of the best fit for the recovery phase show that the values of τ may differ drastically from those corresponding to the main phase. The values of the decay parameter for the main phase of each event, τm, are found to be very sensitive to the adopted efficiency factor, α, decreasing as this factor increases. For the recovery phase, which is characterized by very low values of the power input, the response function becomes almost independent of the value of α and the resulting values for the decay time parameter, τr, do not vary greatly as α varies. As a consequence, the relative values of τ between the main and the recovery phase, τm/τr, can be greater or smaller than one as α varies from 0.1 to 1.

Notes: Abstract A procedure for the mass propagation of pineapple plants (Ananas comosus L. Merr) using a temporary immersion technique is described. This procedure involved three distinct phases in the automated temporary immersion system: shooting, bud differentiation and elongation. To establish this protocol, we used in vitro shoots obtained from established liquid culture as starting materials. Three culture methods (solid, liquid and temporary immersion) were compared. Temporary immersion increased the multiplication rate and fresh and dry weight after 42 days. Conventional micropropagation (liquid medium) and temporary immersion were compared in combination with paclobutrazol. Paclobutrazol promoted the formation of compact bud clusters with limited leaf development. The highest multiplication rate (106) was found when ex-plants were cultured in shooting medium (MS+2.1 mg/l BA+0.3 mg/l NAA) supplemented with 1 mg/l PB for 7 weeks. A 10-l temporary immersion bioreactor was used to test two approaches during elongation stage: reduction of the shoot-formation period or decrease of the initial number of explants. The highest number of competent and uniform plants (191.8 plant/l) was achieved when shoots were cultured for 4 weeks in shooting medium supplemented with PB.

Notes: Abstract  Interleukin-8 is a chemokine with a potent neutrophil chemoatractant activity. In humans, two different cDNAs encoding human IL8 receptors designated IL8RA and IL8RB have been cloned. IL8RA binds IL8, while IL8RB binds IL8 as well as other α-chemokines. Both human IL8Rs are encoded by two genes physically linked on chromosome 2. The IL8RA and IL8RB genes have open reading frames (ORF) lacking introns. By direct sequencing of the polymerase chain reaction products, we sequenced the IL8R genes of cell lines from four non-human primates: chimpanzee, gorilla, orangutan, and macaca. The IL8RB encodes an ORF in the four non-human primates, showing 95% – 99% similarity to the human IL8RB sequence. The IL8RA homologue in gorilla and chimpanzee consisted of two ORF 98% – 99% identical to the human sequence. The macaca and orangutan IL8RA homologues are pseudogenes: a 2 base pair insertion generated a sequence with several stop codons. In addition, we describe the physical linkage of these genes in the four non-human primates and discuss the evolutionary implications of these findings.

Notes: Abstract Interleukin-8 is a chemokine with a potent neutrophil chemoatractant activity. In humans, two different cDNAs encoding human IL8 receptors designated IL8RA and IL8RB have been cloned. IL8RA binds IL8, while IL8RB binds IL8 as well as other α-chemokines. Both human IL8Rs are encoded by two genes physically linked on chromosome 2. TheIL8RA andIL8RB genes have open reading frames (ORF) lacking introns. By direct sequencing of the polymerase chain reaction products, we sequenced theIL8R genes of cell lines from four non-human primates: chimpanzee, gorilla, orangutan, and macaca. TheIL8RB encodes an ORF in the four non-human primates, showing 95%–99% similarity to the human IL8RB sequence. TheIL8RA homologue in gorilla and chimpanzee consisted of two ORF 98%–99% identical to the human sequence. The macaca and orangutanIL8RA homologues are pseudogenes: a 2 base pair insertion generated a sequence with several stop codons. In addition, we describe the physical linkage of these genes in the four non-human primates and discuss the evolutionary implications of these findings.

Notes: Abstract Around solar maximum, the dominant interplanetary phenomena causing intense magnetic storms (Dst〈−100 nT) are the interplanetary manifestations of fast coronal mass ejections (CMEs). Two interplanetary structures are important for the development of storms, involving intense southward IMFs: the sheath region just behind the forward shock, and the CME ejecta itself. Whereas the initial phase of a storm is caused by the increase in plasma ram pressure associated with the increase in density and speed at and behind the shock (accompanied by a sudden impulse [SI] at Earth), the storm main phase is due to southward IMFs. If the fields are southward in both of the sheath and solar ejecta, two-step main phase storms can result and the storm intensity can be higher. The storm recovery phase begins when the IMF turns less southward, with delays of ≈1–2 hours, and has typically a decay time of 10 hours. For CMEs involving clouds the intensity of the core magnetic field and the amplitude of the speed of the cloud seems to be related, with a tendency that clouds which move at higher speeds also posses higher core magnetic field strengths, thus both contributing to the development of intense storms since those two parameters are important factors in genering the solar wind-magnetosphere coupling via the reconnection process. During solar minimum, high speed streams from coronal holes dominate the interplanetary medium activity. The high-density, low-speed streams associated with the heliospheric current sheet (HCS) plasma impinging upon the Earth's magnetosphere cause positive Dst values (storm initial phases if followed by main phases). In the absence of shocks, SIs are infrequent during this phase of the solar cycle. High-field regions called Corotating Interaction Regions (CIRs) are mainly created by the fast stream (emanating from a coronal hole) interaction with the HCS plasma sheet. However, because the Bz component is typically highly fluctuating within the CIRs, the main phases of the resultant magnetic storms typically have highly irregular profiles and are weaker. Storm recovery phases during this phase of the solar cycle are also quite different in that they can last from many days to weeks. The southward magnetic field (Bs) component of Alfvén waves in the high speed stream proper cause intermittent reconnection, intermittent substorm activity, and sporadic injections of plasma sheet energy into the outer portion of the ring current, prolonging its final decay to quiet day values. This continuous auroral activity is called High Intensity Long Duration Continuous AE Activity (HILDCAAs). Possible interplanetary mechanisms for the creation of very intense magnetic storms are discussed. We examine the effects of a combination of a long-duration southward sheath magnetic field, followed by a magnetic cloud Bs event. We also consider the effects of interplanetary shock events on the sheath plasma. Examination of profiles of very intense storms from 1957 to the present indicate that double, and sometimes triple, IMF Bs events are important causes of such events. We also discuss evidence that magnetic clouds with very intense core magnetic fields tend to have large velocities, thus implying large amplitude interplanetary electric fields that can drive very intense storms. Finally, we argue that a combination of complex interplanetary structures, involving in rare occasions the interplanetary manifestations of subsequent CMEs, can lead to extremely intense storms.

Notes: Abstract Cattle tick infestations constitute a major problem for the cattle industry in tropical and subtropical regions of the world. Traditional control methods have been only partially successful, hampered by the selection of chemical-resistant tick populations. The Boophilus microplus Bm86 protein was isolated from tick gut epithelial cells and shown to induce a protective response against tick infestations in vaccinated cattle. Vaccine preparations including the recombinant Bm86 are used to control cattle tick infestations in the field as an alternative measure to reduce the losses produced by this ectoparasite. The principle for the immunological control of tick infestations relies on a polyclonal antibody response against the target antigen and, therefore, should be difficult to select for tick-resistant populations. However, sequence variations in the Bm86 locus, among other factors, could affect the effectiveness of Bm86-containing vaccines. In the present study we have addressed this issue, employing data obtained with B. microplus strains from Australia, Mexico, Cuba, Argentina and Venezuela. The results showed a tendency in the inverse correlation between the efficacy of the vaccination with Bm86 and the sequence variations in the Bm86 locus (R2 = 0.7). The mutation fixation index in the Bm86 locus was calculated and shown to be between 0.02 and 0.1 amino acids per year. Possible implications of these findings for the immunoprotection of cattle against tick infestations employing the Bm86 antigen are discussed.