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  • 1
    ISSN: 1573-4986
    Keywords: influenza virus ; hemagglutinin ; glycoprotein glycans ; oligosaccharide structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Fowl plague virus, strain Dutch, was metabolically labeled withd-[2-3H]mannose, or withd-[6-3H]glucosamine, and the small subunit (HA2; 0.8 mg in total) of the viral hemagglutinin was isolated by preparative sodium dodecylsulfate-polyacrylamide gel electrophoresis. After proteolytic digestion, the radioactive oligosaccharides were sequentially liberated from the glycopeptides by treatment with different endo-β-N-acetylglucosaminidases and with peptide:N-glycosidase or, finally, by hydrazinolysis. In this manner, four groups of glycans could be obtained by consecutive gel filtrations and were subfractionated by HPLC. The structures of the individual oligosaccharides were analyzed by micromethylation, by acetolysis or by digestion with exoglycosidases. The major species amongst the high mannose glycans at Ans-406 of the viral glycopolypeptide were found to be Manα1-2Manα1-3(Manα1-2Manα1-6)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNacβ1-4GlcNAc and Manα1-3(Manα1-2Manα1-6)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc, while the complex glycans at Asn-478 are predominantly GlcNAcβ1-2Manα1-3(GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAc (lacking, in part, one of the outerN-acetylglucosamine residues) and GlcNAcβ1-2Manα1-3(Galβ1-4GlcNAcβ1-2Manα1-6)Manβ1-4GlcNAcβ1-4GlcNAc.
    Type of Medium: Electronic Resource
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  • 2
  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Pure and applied geophysics 116 (1978), S. 181-197 
    ISSN: 1420-9136
    Keywords: Electromagnetic field ; Vertical magnetic dipole ; Thin conductive sheet ; Lateral conductivity variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract An analytical formulation is developed for the resultant electromagnetic field of an oscillating vertical magnetic dipole located over a thin conductive sheet of infinite extent. The sheet is characterized by a conductivity-thickness product or conductance σd that may be a function of the horizontal coordinates. The system of integral equations arising in the general formulation is simplified greatly when azimuthal symmetry prevails. Numerical results for a Gaussian variation of σd in the radial direction are presented for the case of a symmetrically located source. These results are for the fields at the level of the source dipole over the conductive sheet. It is shown that the quadrature response of the sheet is enhanced when there is rapid variation of the conductance. The null in the resultant wave tilt is also found to be shifted toward the direction of increasing conductance.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Manuscripta mathematica 22 (1977), S. 87-103 
    ISSN: 1432-1785
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract This note continues the investigations of Knebusch on algebraic curves over real closed fields and was initiated by reading [3]. Especially we ask for the existence of real algebraic functions with given zeroes and poles, a question going back to Witt [4]. We study the real nature of coverings of real algebraic curves, and if the covering has degree two, we get algebraic proofs for results, which in the classical case have been obtained by topological methods in [2].
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Mathematische Annalen 228 (1977), S. 101-111 
    ISSN: 1432-1807
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
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  • 6
    ISSN: 1573-9333
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics , Physics
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 34 (1978), S. 363-364 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Rapid loss of cochlear microphonics in guinea-pigs previously exposed to high-energy impulse noise was shown to be related to the breakdown of the endolymphatic boundary. The cochlear duct was rendered leaky by deterioration of the reticular membrane, and damage of sensory and supporting cells.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 50 (1977), S. 261-270 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung An der Erythrocytenmembran wird die Beziehung zwischen anisotroper Toluidinblaufärbung und Membranstruktur untersucht. Enzymatischer oder chemischer Abbau der Glykokalyx stört die topo-optische Färbung. Eine Abschwächung wird auch durch Digitoninbehandlung Glutaraldehyd-fixierter Zellen erzielt. Durch Chloroform-Methanol-Inkubation wird die anisotrope Toluidinblaufärbung gelöscht, aber sie kann nach Aldehyd-Bisulfitbildung wieder nachgewiesen werden. An agglutinierten Erythrocyten ist die Wirkung einer Chloroform-Methanol-Behandlung stark eingeschränkt. Die reversibel kollabierte Glykokalyx zeigt keine topo-optische Färbung. Die Befunde werden als Hinweis auf die Bedeutung der räumlichen Orientierung der Glykoproteine an der Zelloberfläche gedeutet. Störungen im Aufbau der Glykokalyx durch Hydrolyse bzw. Verlust oder durch Umverteilung von Komponenten verändern die Bedingungen für die anisotrope Toluidinblaufärbung. Eingriffe an der Lipidschicht können mit solchen Veränderungen einhergehen; für die Interkalation von Farbstoffmolekülen in die Lipidschicht ergab sich jedoch kein Anhalt. Die topo-optische Färbung mit Toluidinblau wird auf Grund dieser Ergebnisse als geeignetes Verfahren zur Untersuchung der Glykokalyx von Erythrocyten (und vermutlich auch anderer Zellen) angesehen.
    Notes: Summary The study was concerned with the molecular structure of the erythrocyte membrane and its anisotropic staining with toluidine blue. Enzymatic or chemical degradation impairs the topo-optical staining. Decreased anisotropy results also from digitonin treatment of glutaraldehyde fixed cells. Incubation in a mixture of chloroform and methanol abolishes the anisotropic staining with toluidine blue, but it has been restored by the aldehyde bisulfite-procedure. The efficacy of a treatment with chloroform-methanol is greatly reduced in the case of agglutinated erythrocytes. The reversibly collapsed glycocalyx is devoid of topo-optical staining. The findings refer to the spatial orientation of glycoproteins at the cell surface. Degradation or loss and rearrangement, respectively, change the glycocalyx structure and likewise deteriorate the conditions for the anisotropic staining. Alterations of the lipid layer of the membrane may result in similar effects. There was, however, no indication of an intercalation into the lipid layer of dyestuff molecules. In conclusion, the topo-optical toluidine blue staining is considered a powerful method aiding studies of the glycocalyx of erythrocytes (and presumably of other types of cells too).
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 26 (1985), S. 591-600 
    ISSN: 1434-6052
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A new variant of the nonlocal light-cone expansion in QCD is proposed. The anomalous dimension of the corresponding fermion flavour nonsinglet operator is calculated in the one-loop approximation and shown to coincide (up to a trivial diagonal factor) with the Brodsky-Lepage kernel. The connection of our approach with the standard local conformal light-cone expansion is briefly discussed. An exclusive light-cone dominated process is considered within the framework of our formalism. The evolution equation obtained earlier by different methods is recovered in a particularly straightforward and compact manner.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 33 (1986), S. 275-280 
    ISSN: 1434-6052
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Meson and baryon operators on the light cone are studied which reproduce the well-known parton distribution amplitudes as matrix elements between the vacuum and an one-meson (baryon) state. We extent our previous results to the case of the quark-gluon mixing which is relevan for the description of a flavour-singlet meson. The anomalous dimensions of the considered nonlocal light-cone operators are calculated explicity in the one-loop approximation. We thus recover the evolution kernels for meson and baryon wave functions derived earlier by other authors using different methods.
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