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  • PANGAEA  (8)
  • American Society for Microbiology  (6)
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  • 1
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    Experiment on marine fungus Microascus brevicaulis strain: Comparative growth curves of LF580 and LF580-M26 (2015)
    PANGAEA
    Details
    Publication Date: 2023-03-14
    Keywords: Biomass; Biomass, standard error; Experiment day; pH; pH, standard deviation; Species; Strain; Time in hours
    Type: Dataset
    Format: text/tab-separated-values, 288 data points
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    DATA PANGAEA
  • 2
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    Physico-chemical parameters and accession numbers from the Logatchev Hydrothermal Field, located 15°N on the Mid-Atlantic Ridge (2021)
    PANGAEA
    Details
    Publication Date: 2023-03-15
    Description: Hydrothermal fluid samples were retrieved during dives made by the remotely operated vehicle (ROV) QUEST (MARUM, University of Bremen) during the HYDROMAR I (M60/3, 2004) and HYDROMAR II (M64/2, 2005) cruises to the Logatchev hyrothermal field. The samples were retrieved using a pumped flow-through system (Kiel Pumping System KIPS) specially designed for the ROV QUEST. Once on board the ship, the liquids intended for microbiological studies were concentrated on 0.2 µm pore size polycarbonate filters (Sartorius) and stored at -20°C. The pH and sulfide concentrations were determined immediately after sample recovery. The pH was measured (Mettler electrodes with Ag/AgCl reference electrode) at 25°C in unfiltered sample aliquots. Sulfide concentrations were determined photometrically following the methylene blue method or, for samples with low concentrations, by voltammetry (Metrohm Application Bulletin 199/3e). Methane was analyzed on board by applying a purge and trap technique. For on-board measurements of dissolved hydrogen the water sample was degassed into a high-grade vacuum. Aliquots of the released gas were analyzed by gas chromatography (Thermo Electron Corporation Trace GC Ultra with a pulsed discharge detector). The abundance of bacterial and archaeal taxa was investigated by sequencing of the 16S rRNA gene. The diversity of the cbbL, cbbM and aclb was investigated by sequencing of the genes.
    Keywords: Accession number, genetics; ANME-2; Area/locality; chemoautotrophy; Epsilonproteobacteria; fluid chemistry; Gene name; Hydrogen; hydrogen oxidation; Hydrogen sulfide; LHF; Logatchev_Hydrothermal-Field; Logatchev hydrothermal field; Methane; Methanococcales; Mid-Atlantic Ridge; pH; Remote operated vehicle QUEST; ROVQ; sulfur oxidation; Temperature, water; ultramafic-hosted
    Type: Dataset
    Format: text/tab-separated-values, 26 data points
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    DATA PANGAEA
  • 3
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    Experiment on marine fungus Microascus brevicaulis strain (2015)
    PANGAEA
    In:  Supplement to: Kramer, Annemarie; Beck, Hans Christian; Kumar, Abhishek; Kristensen, Lars Peter; Imhoff, Johannes F; Labes, Antje (2015): Proteomic Analysis of Anti-Cancerous Scopularide Production by a Marine Microascus brevicaulis Strain and Its UV Mutant. PLoS ONE, 10(10), e0140047, https://doi.org/10.1371/journal.pone.0140047
    Details
    Publication Date: 2023-01-13
    Description: The marine fungus Microascus brevicaulis strain LF580 is a non-model secondary metabolite producer with high yields of the two secondary metabolites scopularide A and B, which exhibit distinct activities against tumour cell lines. A mutant strain was obtained using UV mutagenesis, showing besides higher production levels faster growth and differences in pellet formation. Comparative proteomics were applied to gain deeper understanding of the regulation of production and of the physiology of this fungus and its mutant. For this purpose, an optimised protein extraction protocol was established. Here, we show the first proteome study of a marine fungus. In total, 4759 proteins were identified. The central metabolic pathway of LF580 could be mapped by using KEGG pathway analysis and GO annotation. Using iTRAQ labelling, 318 proteins were shown to be significantly regulated in the mutant strain: 189 were down- and 129 upregulated. Proteomics are a powerful tool for the understanding of regulatory aspects: The differences on proteome level could be attributed to a limited nutrient availability in wild type strain due to a strong pellet formation. This information can be applied to optimisation on strain and process level. The linkage between nutrient limitation and pellet formation in the non-model fungus M. brevicaulis is in consensus with the knowledge on model organisms like Aspergillus niger and Penicillium chrysogenum.
    Type: Dataset
    Format: application/zip, 2 datasets
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  • 4
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    NMR spectrum of lindgomycin from a Marine Fungus of the Lindgomycetaceae (2016)
    PANGAEA
    In:  Supplement to: Wu, Bin; Wiese, Jutta; Labes, Antje; Kramer, Annemarie; Schmaljohann, Rolf; Imhoff, Johannes F (2015): Lindgomycin, an Unusual Antibiotic Polyketide from a Marine Fungus of the Lindgomycetaceae. Marine Drugs, 13(8), 4617-4632, https://doi.org/10.3390/md13084617
    Details
    Publication Date: 2023-01-13
    Description: An unusual polyketide with a new carbon skeleton, lindgomycin (1), and the recently described ascosetin (2) were extracted from mycelia and culture broth of different Lindgomycetaceae strains, which were isolated from a sponge of the Kiel Fjord in the Baltic Sea (Germany) and from the Antarctic. Their structures were established by spectroscopic means. In the new polyketide, two distinct domains, a bicyclic hydrocarbon and a tetramic acid, are connected by a bridging carbonyl. The tetramic acid substructure of compound 1 was proved to possess a unique 5-benzylpyrrolidine-2,4-dione unit. The combination of 5-benzylpyrrolidine-2,4-dione of compound 1 in its tetramic acid half and 3-methylbut-3-enoic acid pendant in its decalin half allow the assignment of a new carbon skeleton. The new compound 1 and ascosetin showed antibiotic activities with IC50 value of 5.1 (±0.2) µM and 3.2 (±0.4) µM, respectively, against methicillin-resistant Staphylococcus aureus.
    Type: Dataset
    Format: application/pdf, 49.3 kBytes
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  • 5
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    Experiment on marine fungus Microascus brevicaulis strain: Overview of Gene Ontology data of mutant strain LF580-M26 in comparision to wild type strain LF580 (2015)
    PANGAEA
    Details
    Publication Date: 2023-01-13
    Keywords: Accession number; Category; Comment; Description; Enzyme code; Gene Ontology term
    Type: Dataset
    Format: text/tab-separated-values, 1828 data points
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  • 6
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    (Table 2) Hydrogen sulphide and methane content, and loss on ignition in sediments obtained in the Gotland Deep, Baltic Sea
    PANGAEA
    In:  Supplement to: Schmaljohann, Rolf; Piker, Levent; Imhoff, Johannes F (1998): The distribution of methane and hydrogen sulfide in basin sediments of the central and southern Baltic Sea. Meyniana, 50, 191-211, https://doi.org/10.2312/meyniana.1998.50.191
    Details
    Publication Date: 2023-05-12
    Description: The distribution of methane and hydrogen sulfide concentrations in sediments of various basins of the Baltic Sea was investigated during 4 cruises in 1995 and 1996. Significant differences in the concentrations of both compounds were recorded between the basins and also between different areas within the Gotland Deep. High-methane sediments with distinctly increasing concentrations from the surface to deeper layers were distinguished from low-methane sediments without a clear gradient. Methane concentrations exhibited a fair correlation with the sediment accumulation rate, determined by measuring the total thickness of the post-Ancylus Holocene sequence on echosounding profiles in the Gotland Deep. Only weak correlations were observed with the content of organic matter in the surface layers of the sediments. Hydrogen sulfide concentrations in the sediments showed a positive correlation with methane concentrations, but, in contrast to methane concentrations, were strongly influenced by the transition from oxic to anoxic conditions in the water column between 1995 and 1996. Sediments in the deepest part of the Gotland Basin (〉237 m water depth), covering an area of approximately 35 km**2, were characterized by especially high accumulation rates (〉70 cm/ka) and high methane and hydrogen sulfide contents. Concentrations of these compounds decreased rapidly towards the slope of the basin.
    Keywords: AL93; Arcona Basin; BY15A; Date; Depth, bottom/max; DEPTH, sediment/rock; Depth, top/min; Direction; Distance; Elevation 2; Event label; GC; GotlDeep; Gravity corer; Hydrogen sulfide; Layer thickness; Loss on ignition; Methane, sediment; Sample code/label
    Type: Dataset
    Format: text/tab-separated-values, 613 data points
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  • 7
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    Physico-chemical parameters and accession numbers from the Lilliput hydrothermal field, southern Mid-Atlantic Ridge (9°S) (2021)
    PANGAEA
    Details
    Publication Date: 2024-02-02
    Description: Hydrothermal vent fluids were sampled with a pumped flow-through system with PFA sampling bottles (Kiel Pumping System KIPS) by the ROV QUEST. Fuid chemical parameters were determined immediately after sample recovery. Ph was measured at 20°C in unfiltered sample aliquots. Sulfide concentrations were measured colorimetrcally following the methylene blue method. Fe(II) and Fe(III) was measured colorimetrically with the phenantroline assay. Total Fe was measured by reducing all Fe(III) to Fe(II) with ascorbic acid. Fe(III) was calculated from the diffrence between Fe(II) and total Fe. Light dissolved hydrocharbons were analysed on board applying a purge and trap technique. Dissolved hydrogen was mesured onboard by a GC equipped with a PDD. Stable carbon isotope signatures of methane were analysed using coupled gas chromatography-combustion-isotope ration mass spectrometry. The abundance of bacterial and archaeal taxa was investigated by sequencing of the 16S rRNA gene. The diversity of the cbbL, cbbM and aclb was investigated by sequencing of the genes.
    Keywords: Accession number, genetics; Area/locality; Candidate Divisions; Endmember fluid portion; Epsilonproteobacteria; fish; Gene name; Hydrogen; Hydrogen sulfide; Iron 2+; Iron 3+; low-temperature hydrothermal fluids; M64/1; M64/1_Lilliput-hydrothermal-field; M68/1; M68/1_Lilliput-hydrothermal-field; MARSUED2; MARSUED3; Meteor (1986); Methane; microbial community; Mid-Atlantic Ridge; pH; Remote operated vehicle QUEST; ROVQ; Sampling date; sulfur oxidation; Temperature, water; Thiomicrospira; δ13C, methane
    Type: Dataset
    Format: text/tab-separated-values, 42 data points
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  • 8
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    Betaine is the main compatible solute of halophilic eubacteria (1984)
    American Society for Microbiology
    In:  Journal of Bacteriology, 160 (1). pp. 278-479.
    Details
    Publication Date: 2016-01-22
    Description: A number of moderately halophilic bacteria of diverse taxonomic groups have been studied to determine the intracellular concentrations of organic compounds at various salt concentrations. Betaine was accumulated in all of these organisms in proportion to the salinity of the medium, suggesting that this compound plays a major role in osmoregulation.
    Type: Article , PeerReviewed
    Format: text
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  • 9
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    Genome Sequence of Bacillus subtilis subsp. spizizenii gtP20b, Isolated from the Indian Ocean (2011)
    American Society for Microbiology
    In:  Journal of Bacteriology, 193 (5). pp. 1276-1277.
    Details
    Publication Date: 2019-09-23
    Description: Bacillus subtilis is an aerobic spore-forming Gram-positive bacterium that is a model organism and of great industrial significance as the source of diverse novel functional molecules. Here we present, to our knowledge, the first genome sequence of Bacillus subtilis strain gtP20b isolated from the marine environment. A subset of candidate genes and gene clusters were identified, which are potentially involved in production of diverse functional molecules, like novel ribosomal and nonribosomal antimicrobial peptides. The genome sequence described in this paper is due to its high strain specificity of great importance for basic as well as applied researches on marine organisms
    Type: Article , PeerReviewed
    Format: text
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  • 10
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    Bch-Y-based degenerate primers target all types of anoxygenic photosynthetic bacteria in a single PCR reaction (2009)
    American Society for Microbiology
    In:  Applied and Environmental Microbiology, 75 . pp. 7556-7559.
    Details
    Publication Date: 2019-09-23
    Description: To detect anoxygenic bacteria containing either type 1 or type 2 photosynthetic reaction centers in a single PCR, we designed a degenerate primer set based on the bchY gene. The new primers were validated in silico using the GenBank nucleotide database as well as by PCR on pure strains and environmental DNA.
    Type: Article , PeerReviewed
    Format: text
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