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  • Oxford University Press  (208)
  • Wiley-Blackwell
  • 1
    Electronic Resource
    Electronic Resource
    Single-cell protein from methanol with Enterobacter aerogenes (1987)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 29 (1987), S. 355-357 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An identifiedEnterobacter aerogenesutilizing methanol as a sole carbon source was studied for the optimization of biomass production and the reduction of its nucleic acid content. Results indicated that the highest yield and conversion were obtained at 0.5% methanol. The addition of seawater as a source of trace elements has an adverse effect. However, the addition of urea as source of nitrogen enhanced the growth of E. aerogenes. Heat shock at 60°C for 1 min followed by incubation at 50°C for 2 h caused 72.6% reduction in the nucleic acid.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260290310
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  • 2
    Electronic Resource
    Electronic Resource
    Simulation of an electrochemical carbon dioxide concentrator (1976)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 22 (1976), S. 1042-1050 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The performance of an electrochemical device for the concentration of carbon dioxide from the atmosphere of a spacecraft cabin is analyzed. The removal rates as well as the concentration distributions of the different species are calculated for any set of operating conditions by a model which embodies the fundamental electrolyte properties and design parameters.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690220614
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  • 3
    Electronic Resource
    Electronic Resource
    Stereochemical influences upon the opioid ligand activities of 4-alkyl-4-arylpiperidine derivatives (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Chirality 1 (1989), S. 202-208 
    Details
    ISSN: 0899-0042
    Keywords: opioid ligand ; 4-arylpiperidines ; conformation, NMR ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The synthesis and stereochemistry (configuration and preferred solute conformation) of some 4-alkyl (methyl, n-propyl, isobutyl)-4-(3-hydrxyphenyl)-1-methylpiperidines and corresponding 3-methyl diastereoisomeric pairs are reported, together with their in vivo and in vitro activities as opioid ligands. All potent agonists exhibit a preference for axial 4-aryl chair conformations when protonated, and stereochemical analogies with rigid opioids of the benzomorphan class are discussed. Antagonist properties are found in compounds with preference for equatorial 4-aryl chairs, notably the cis 3,4-dimethyl derivative.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/chir.530010305
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  • 4
    Electronic Resource
    Electronic Resource
    Selection of salt hydrate pairs for use in water control in enzyme catalysis in organic solvents (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 55 (1997), S. 367-374 
    Details
    ISSN: 0006-3592
    Keywords: salt hydrates ; water activity ; subtilisin ; lipase ; organic solvents ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The water activities (aw) of 13 salt hydrate pairs were determined from vapor pressure measurements; aw values for a subset were also estimated from a study of water transfer to isopropylether. The application of salt hydrates as water buffers was investigated in two models: (i) effect of hydration on the initial rate of subtilisincatalyzed transesterification of the nitrophenol ester of CBZ-alanine with butanol; and (ii) effect of hydrates on the equilibrium concentrations of reactants in the esterification of dodecanol and decanoic acid, catalyzed by lipase. Transfer of ions from salt to enzyme particles was also demonstrated. The implications of the results for the successful use of salt hydrates as water buffers are discussed. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55: 367-374, 1997.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Correlation between the distribution of smooth muscle or non muscle myosins and α-smooth muscle actin in normal and pathological soft tissues (1988)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 11 (1988), S. 260-274 
    Details
    ISSN: 0886-1544
    Keywords: cytoskeleton ; atheromatosis ; wound healing ; fibromatosis ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The distribution of smooth muscle (SM) and non muscle myosins was compared with that of α-SM actin in various normal and pathological tissues and in cultured cells by means of indirect immunofluorescence using a monoclonal antibody specific for α-SM actin [anti-αsm-1, Skalli et al., 1986b] and two polyclonal antibodies raised against bovine aortic myosin (ABAM) and human platelet myosin (AHPM), respectively.In normal tissues ABAM stained vascular and parenchymal smooth muscle cells (SMC), myoepithelial cells and myoid cells of the testis in a pattern similar to that reported by other authors with antisera raised against non vascular SM myosin. Cells stained with ABAM were always positive for anti-αsm-1. In human and experimental atheromatous plaques, most cells were positive for AHPM; a variable proportion was also stained for ABAM plus anti-αsm-1. Myofibroblasts from rat granulation tissue, Dupuytren's nodule and stroma from breast carcinoma were constantly positive for AHPM and negative for ABAM; however, myofibroblasts from Dupuytren's nodule and breast carcinoma were anti-αsm-1 positive. Early primary cultures of rat aortic SMC were positive for ABAM and anti-αsm-1 and became negative for ABAM and positive for AHPM after a few days in culture. They remained positive for AHPM and anti-αsm-1 after passages; the staining of AHPM and anti-αsm-1 appeared to be colocalized along the same stress fibers.These results may be relevant for the understanding of SMC function and adaptation, and show that in non malignant SMC proliferation, α-SM actin represents a more general marker of SM origin than SM myosin.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970110405
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  • 6
    Electronic Resource
    Electronic Resource
    [4 + 2]-Cycloaddition of Singlet Oxygen and Phenyltriazolinedione with Bicyclo[6.1.0]nona-2,4,6-triene and Derivatives (1982)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 115 (1982), S. 1170-1177 
    Details
    ISSN: 0009-2940
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: [4+2]-Cycloaddition von Singulett-Sauerstoff und Phenyltriazolindion an Bicyclo[6.1.0]nona-2,4,6-trien und seine DerivateDie Dienreaktivität der Bicyclotriene 1a ([4.3.0]-Isomeres) und 1b ([6.1.0]-Isomeres), von dessen Oxaderivat 6 (Cyclooctatetraenepoxid) sowie von 1,3,5-Cyclooctatrienon (7) gegenüber Singulett-Sauerstoff (1O2) und Phenyl-1,2,4-triazolin-3,5-dion (PTAD) als Dienophile wurde untersucht. Bicyclononatrien 1a liefert das Endoperoxid 9a in 60proz. Ausbeute. Die anderen Substrate 1b, 6 und 7 reagieren nicht mit 1O2. Mit PTAD dagegen wurden aus 1a, 1b, 6 und 7 die Urazole 10a, 8b, 12c und 11 gebildet. Ungewöhnlich ist, daß das Bicyclo[6.1.0]trien 1b nur zum tricyclischen Urazol 8b führt, dem ersten Beispiel solcher [4 + 2]-Cycloaddukte von 1b, während die analoge Oxaverbindung 6 nur tetracyclisches Urazol 12c gibt. Dabei ist zu bemerken, daß 6 mit PTAD beträchtlich träger reagiert als 1b.
    Notes: The dienic reactivity of the bicyclononatrienes 1a ([4.3.0]-isomer) and 1b ([6.1.0]-isomer) and its oxa-derivative 6 (cyclooctatetraene epoxide) as well as 1,3,5-cyclooctatrienone (7), towards singlet oxygen (1O2) and phenyl-1,2,4-triazoline-3,5-dione (PTAD) as dienophiles has been investigated. Except for 1a, which affords the endoperoxide 9a in 60% yield, the remaining substrates 1b, 6, and 7 do not react with 1O2. With PTAD 1a, 1b, 6, and 7 afford the urazoles 10a, 8b, 12c, and 11, respectively. Unusual is the fact that the bicyclo[6.1.0]triene 1b leads only to the tricyclic urazole 8b, the first example of this type of [4 + 2]-cycloadduct of 1b, while the oxa-analog 6 affords only the tetracyclic urazole 12c. Moreover, 6 reacts with PTAD considerably more sluggishly than 1b.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cber.19821150333
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  • 7
    Electronic Resource
    Electronic Resource
    Time scales and other problems in linking simulations of simple chemical systems to more complex ones (1993)
    New York, NY : Wiley-Blackwell
    International Journal of Quantum Chemistry 45 (1993), S. 511-544 
    Details
    ISSN: 0020-7608
    Keywords: Computational Chemistry and Molecular Modeling ; Atomic, Molecular and Optical Physics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: We shall start with very small systems like H2 and H3, computed with very accurate methods (Hylleraas-CI) or atomic systems up to Zn with accurate methods (CI), then move to more complex ones, like C60, but now with somewhat less accurate methods, specifically Hartree-Fock with density functionals, the latter for the correlation energy but not for the exchange energy. For even more complex tasks like geometry optimization of C60, we have resorted to even simpler and parametrized methods, like local density functionals. Then, we could use quantum mechanics either to provide interaction potentials for classical molecular dynamics or to directly solve dynamical systems, in a quantum molecular dynamics approximation. Having demonstrated that we can use the computational output from small systems as input to larger ones, we discuss in detail a new model for liquid water, which is borne out entirely from ab initio methods and nicely links spectroscopic, thermodynamics, and other physicochemical data. Concerning time scales, we use classical molecular dynamics to determine friction coefficients, and with these we perform stochastic dynamic simulations. The use of simulation results from smaller systems to provide inputs for larger system simulations is the “global simulation” approach, which, today, with the easily available computers, is becoming more and more feasible. Projections on simulations in the 1996-1998 period are discussed, new computational areas are outlined, and a N4 complexity algorithm is compared to density functional approaches. © 1993 John Wiley & Sons, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/qua.560450605
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  • 8
    Electronic Resource
    Electronic Resource
    Binding of chemotactic peptide to the outer surface and to whole human spermatozoa with different affinity states (1988)
    New York, NY : Wiley-Blackwell
    Gamete Research 20 (1988), S. 233-239 
    Details
    ISSN: 0148-7280
    Keywords: human sperm ; membrane ; receptors ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Binding of N-formyl-methionyl-L-leucyl-[3H]phenylalanine (fML[3H]Ph) to human ejaculated spermatozoa and to its isolated plasma membrane was studied. Our data confirm the presence of specific receptors for f-MLPh in the human spermatozoa and suggest that whole spermatozoa receptors exist in two affinity states, one high-affinity, low-capacity specific receptor (Kd = 12.3 ± 0.5 nM, n = 22,285 ± 65,008 binding sites per sperm cell) and a second one (Kd = 700 ± 47 nM) that is not saturable, indicating a low-affinity, high-capacity nonspecific site. In contrast, sperm membrane showed only one class of binding site (Kd = 6.4 ± 0.12 nM), which was statistically different from that of the high-affinity binding site of intact spermatozoa. To explain this difference we discuss the possibility that first, the two binding affinities represent two interconvertible states of a single receptor population, which, depending on the metabolic activity of spermatozoa, may change its physicochemical properties; or second, they reflect two different processes, binding and/or transport into the spermatozoa.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120200213
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  • 9
    Electronic Resource
    Electronic Resource
    Heparin and glutathione: Physiological decondensing agents of human sperm nuclei (1989)
    New York, NY : Wiley-Blackwell
    Gamete Research 23 (1989), S. 39-47 
    Details
    ISSN: 0148-7280
    Keywords: nuclei decondensation ; thiol reagents ; reduced glutathione ; glycosaminoglycan ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: It has been proposed that reduced glutathione (GSH) or other thiol reagents may partici pate in the basic mechanism by which sperm-decondensing activity is accomplished. How ever, in vitro, these reagents seem to be inactive and require the presence of other chemi cals, usually detergents. Heparin binds specifically to the sperm membrane and provokes the decondensation of human sperm and the activation of DNA transcription and synthe sis. However, the concentrations at which these effects occur seem to be higher than those expected under physiological conditions. In the present study, thiol reagents at 10 mM concentration, either alone or combined, were completely ineffective in inducing any sig nificant nuclear decondensation after prolonged exposures (24 hr) of incubation. Heparin, 153.8 μM, was capable of inducing only a small increase in nuclear swelling. However, GSH at concentrations as low as 0.1 mM in combination with heparin induces deconden sation of human sperm nuclei in vitro. When GSH concentration was kept constant at 5 mM, nuclear decondensation was induced with heparin at concentrations as low as 11.6 μM, and a maximal decondensation (90%) was obtained with only 21.6 μM of heparin. The latter is more than ten times less than the minimal active concentration of heparin used alone.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120230105
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  • 10
    Electronic Resource
    Electronic Resource
    A rapid screening technique for identifying murine monoclonal antibodies to human apolipoprotein A-I isoforms by Western blot analysis (1987)
    Weinheim : Wiley-Blackwell
    Electrophoresis 8 (1987), S. 552-556 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: A rapid method for determining the reactivity of murine monoclonal antibodies with apolipoprotein A-I (Apo A-I) isoforms has been developed. The method consists of a rapid (1-2 h) prescreening of fusion well culture fluids by using an automated immunofluorescent assay (IF A) to identify fusion well cultures producing monoclonal antibody against Apo A-I and high-density lipoprotein (HDL). Immediately following the IFA procedure, immunodetection of the reactivity pattern of selected fusion well culture fluids is determined by using transblotted isoforms of Apo A-I previously separated by isoelectric focusing. Strips are prepared and stored at 4 °C in protein containing blocking solution before use. Preselected culture fluids could be tested within 6 h for qualitative reactivity with Apo A-I isoforms. Using this procedure we selected several monoclonal antibody-producing cultures for further studies on HDL metabolism based on their differing reactivities with Apo A-I isoforms. This technique should be easily transferable to other laboratories for assessing proteins having multiple isoforms since the crucial steps of separation and blotting can be optimized and performed before actual testing for antibody reactivity.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150081203
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