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  • Blackwell Publishing Ltd  (16)
  • Oxford University Press  (13)
  • 2000-2004  (29)
  • 2000  (29)
  • 1
    ISSN: 1749-7345
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: RNase U2 is an endoribonuclease secreted by the fungus Ustilago sphaerogena. Its genomic DNA (rnu2), containing an intron of 116 bp, has been isolated and cloned. The corresponding cDNA has also been synthesized. The recombinant RNase U2 was successfully produced in Pichia pastoris, fused to the yeast alkaline phosphatase signal peptide. The recombinant RNase U2, purified by affinity chromatography, contains three extra amino acids at its amino-terminal end and retains the enzymatic and spectroscopic properties of the natural fungal protein.
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  • 3
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: We report cloning and sequencing of gene ps1 encoding a versatile peroxidase combining catalytic properties of lignin peroxidase (LiP) and manganese peroxidase (MnP) isolated from lignocellulose cultures of the white-rot fungus Pleurotus eryngii. The gene contains 15 putative introns, and the deduced amino acid sequence consists of a 339-residue mature protein with a 31-residue signal peptide. Several putative response elements were identified in the promoter region. Amino acid residues involved in oxidation of Mn2+ and aromatic substrates by direct electron transfer to heme and long-range electron transfer from superficial residues as predicted by analogy with Phanerochaete chrysosporium MnP and LiP, respectively. A dendrogram is presented illustrating sequence relationships between 29 fungal peroxidases.
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 16 (2000), S. 0 
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. The effects of pig slurry applications to a hydrologically isolated field treatment plant (at Solepur) were studied over a period of eight years. Thirty repeated doses, averaging 160 m3 ha−1 were applied from April to October of each year (1991–1995), to reach a total application of 4930 m3 ha−1. All slurry samples were analysed for their total solids (TS), macronutrient (C, N, P, K, Ca) and micronutrient (Cu, Zn) content. In total, 284 tonnes of total solids (57 t TS ha−1 yr−1), 115 tonnes of carbon (23 t C ha−1yr−1), 24.5 tonnes of nitrogen (4900 kg N ha−1 yr−1), 7964 kg of phosphorus (1593 kg P ha−1 yr−1), 16 518 kg of potassium (3304 kg K ha−1 yr−1), 183 kg copper (37 kg Cu ha−1 yr−1) and 266 kg zinc (53 kg Zn ha−1 yr−1) were applied to the soil. Thus, this site provides an opportunity to assess the balance and to examine the long-term behaviour of nutrients under conditions of intensive land application of pig slurries or similar effluents.The main nutrient fluxes through the soil-water system were determined for each element. Over 40% of the total carbon applied was retained by the soil. About 25% of the slurry nitrogen applied remained in the soil profile and 12.5% was leached through the drainage water as nitrate. Most of the slurry phosphorus applied was retained in the soil profile either as P-Dyer extractable (83%), or as total soil phosphorus (112%); 〈0.01% was found in the drainage water. Forty-three per cent of the potassium applied in the slurry was recovered from the soil profile and 15% was recovered in the drainage water. Most of the copper (62%) and zinc (74%) applied in the slurry remained in the soil as EDTA extractractable forms; very low percentages (0.05% and 0.6% respectively) were found in the drainage water.
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  • 5
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: By including hydrophobic chromatography in the purification scheme, 2 homogeneous tomato fruit peroxidase isoenzymes were obtained. The expression of these 2 peroxidases, one acid and the other basic, was determined in tomato fruits grown under different salinity conditions. Increased salinity modified the isoenzyme profile of tomato peroxidase. In tomatoes grown under highly saline conditions, there was an increase in the expression of the acid form with respect to the basic, the acid/basic ratio rising from 4.5 in tomatoes grown under normal saline conditions to 70 in those grown in highly saline conditions. Kinetic studies using 2,2′-azinobis(3-ethylbenzo-thiazolinesulfonic acid) as reducing substrate showed that increased salinity in the growth medium did not modify the kinetic parameter of tomato peroxidase over both hydrogen peroxide or reducing substrate.
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  • 6
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Peaches (Prunus persica L., cv. Babygold 6) harvested at the stage of commercial ripening were pressure-infiltrated with putrescine (1 mM) or GA3 (100 mg L-1) and then stored at 2°C for 14 d. Both treatments increased fruit firmness, putrescine-treated peaches being significantly firmer than control ones. Treatments were also effective in reducing the susceptibility of the fruit to be damaged by mechanically compression with lower volume and surface of the damage zone. Ethylene emission and the respiration rate were reduced in treated fruits, which reflects a delay of the ripening process. The effects of the mechanical stress could be related to increased spermidine levels, rather than ethylene emission and respiration rate during storage.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 65 (2000), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effect of chemical pretreatment with calcium chloride and ethylene diamine tetraacetic acid (EDTA) in combination with 2-phase crossflow microfiltration and ultrafiltration was studied. Solutions of untreated and chemically treated whey were microfiltered at 3 flow rates, with 0%, 10%, and 20% air injection. Significant increases (p 〈 0.05) in flux were found for untreated and EDTA treated whey at 10 L/min when 20% air was applied. Microfiltration permeates also had a higher (p 〈 0.05) protein concentration at this flow rate when air was used. Ultrafiltration of the microfiltration permeates resulted in no difference (p 〈 0.05) between the flux of untreated permeate ultrafiltered with 20% air and calcium chloride treated whey permeate with 0% air injection. This suggests that 2-phase flow with air injection could be used to increase the efficiency of both the microfiltration and ultrafiltration of whey and chemical pretreatment could be minimized.
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  • 8
    ISSN: 1749-7345
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Two feeding trials were conducted to determine the digestibility of a casein-based semi-purified diet and the effects of different protein levels on growth and protein use of spotted sand bass Paralabrax maculatofasciatus juveniles. For trial I, a semipurified diet with vitamin-free casein as the sole source of protein was fed three times a day to apparent satiation, for a period of 20 d. Feces were collected by siphoning each tank. The digestibility of the experimental diet was high: 97% for protein, 89% for lipids, and 84% for gross energy, whereas that of organic matter was 78%. For trial II, seven diets were formulated using vitamin-free casein at graded levels (25, 30, 35, 40, 45, 50, and 55% protein). Triplicate tanks for each dietary treatment were stocked with fish and fed by hand three times a day to apparent satiation for 6 wk. Perfomance of fish fed the different diets was evaluated for survival, percent weight gain, specific growth rate, feed conversion ratio, and protein efficiency ratio. Survival was 100% for all treatments. Growth of spotted sand bass juveniles increased as the dietary protein increased, but no evidence of reaching a plateau was found. The daily feed intake values showed an inverse relation to the protein content of the diets. The feed conversion ratio did not differ among diets containing 40% protein or greater. The results indicate that spotted sand bass juveniles with 2.5-g mean weight need at least 55% dietary protein for best growth when casein is the sole protein source. However, in terms of feed conversion ratio, the requirement apparently could be lower.
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 119 (2000), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The dwarf safflower mutant ‘Enana’ has been developed from the cultivar ‘Rancho’ by chemical mutagenesis. The objective of the present research was to study the inheritance of plant height in crosses between ‘Enana’ and ‘Rancho’. Plants of both lines were reciprocally crossed and the F1, F2 and F3 generations were obtained. The evaluation of plant height in the F2 generation suggested the presence of a single locus controlling this trait. This was confirmed after the evaluation of 164 F2:3 lines, which revealed an F2 segregation fitting a 1:2:1 (dwarf: intermediate: standard) ratio. The locus was designated Dw. As the mutated allele was partly dominant over the wild-type allele, the proposed genotype for ‘Rancho’ was dw dw, whereas that for ‘Enana’ was Dw Dw. Furthermore, a partial cytoplasmic effect on plant height was detected, with the heterozygote Dw dw being about 6 cm taller when ‘Rancho’ was used as female.
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 119 (2000), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The resistance of 12 apricot cultivars to the Dideron type Spanish isolate RB3.30 of plum pox potyvirus was evaluated in controlled conditions in an insect-proof greenhouse. The results of the evaluation demonstrated the resistance of the cultivars ‘Stella’, ‘Stark Early Orange’, ‘Goldrich’, ‘Harcot’, ‘NJA2’, ‘Pandora’ and ‘Avilara’ to the isolate, and the susceptibility of the cultivars ‘A1453’, ‘Bulida’,‘Currot’, ‘Real Fino’ and ‘Canino’
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