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  • 1
    Electronic Resource
    Electronic Resource
    Selective activation of Lyt 2 + precursor T cells by ligation of the antigen receptor (1985)
    [s.l.] : Nature Publishing Group
    Nature 317 (1985), S. 627-629 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The monoclonal antibody F23.1 recognizes the T-cell antigen receptor on 25% of T cells in most mouse strains1. Intact F23.1 antibody in the presence of accessory cells, and either intact antibody or F(ab')2 fragments coupled to Sepharose beads, are mitogenic in bulk cultures for resting T cells in ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/317627a0
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  • 2
    Electronic Resource
    Electronic Resource
    Transient expression of IL-2 receptor precedes the differentiation of immature thymocytes (1987)
    [s.l.] : Nature Publishing Group
    Nature 329 (1987), S. 157-159 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Adoptive intrathymic transfer of unfractionated adult CD4~8~ thymocytes into irradiated Thy-1 congenic recipients resulted in a wave of donor cell repopulation that peaked at about day 12 in the host thymus and at ~3-4 weeks in host lymph nodes and spleen (Table 1). Early after cell transfer we ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/329157a0
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  • 3
    Electronic Resource
    Electronic Resource
    Differentiation potential of subsets of CD4 − 8 − thymocytes (1987)
    [s.l.] : Nature Publishing Group
    Nature 329 (1987), S. 336-339 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] We studied the surface phenotypes of CD4~8~ thymocyte subpopulations in relation to the haematopoietic differentiation marker Jlld because this marker distinguishes stages in the differentiation of both B and T lymphocytes. In particular, it is expressed on most thymocytes but not on peripheral T ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/329336a0
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  • 4
    Electronic Resource
    Electronic Resource
    Footprints of the Sun (1980)
    [s.l.] : Nature Publishing Group
    Nature 283 (1980), S. 244-245 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] WHAT has the Sun been up to lately? The question is becoming important both scientifically and socially, as more evidence appears that the Sun is (and has been) a variable star. Astronomers worry about the Sun's variability as a clue to understanding stellar behaviour. Everyone else is (or should ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/283244a0
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  • 5
    Electronic Resource
    Electronic Resource
    Two Probable Astroblemes in Brazil (1973)
    [s.l.] : Nature Publishing Group
    Nature 244 (1973), S. 561-562 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The Araguainha Dome, centred at 16 46' S, 52c 59' W, is located near the town of Araguainha in the state of Matto Grosso, Brazil. The structure has been regarded as an alkaline intrusive, of which there are many examples in Brazil. It consists of a central uplift about 10 km across, in which ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/244561a0
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  • 6
    Electronic Resource
    Electronic Resource
    A rapid transformation method for Solanum tuberosum using binary Agrobacterium tumefaciens vectors (1988)
    Springer
    Plant cell reports 7 (1988), S. 13-16 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A tuber disc transformation and regeneration system was devised for potato (Solanum tuberosum). Tuber discs were found to be the most morphogenetic organ on a medium previously optimised for tomato regeneration. Shoot regeneration from tuber discs was rapid and transformed as shown by nopaline assays and Southern blot analysis. The ease and speed of the tuber disc method will allow for the increased use of this commercially important plant in transformation studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272967
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  • 7
    Electronic Resource
    Electronic Resource
    Genetic transformation in two potato cultivars with T-DNA from disarmed Agrobacterium (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 744-750 
    Details
    ISSN: 1432-2242
    Keywords: Genetic manipulation ; Neomycin phosphotransferase ; Mixed infection ; Somaclonal variation ; Solanum tuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Derivatives of potato (Solanum tuberosum cv.'s ‘Maris Bard’ and ‘Desiree’) transformed with disarmed T-DNA from genetically engineered Agrobacterium tumefaciens strains were isolated. The transformed plants were recovered from shoot-forming tumours induced by infection of wounds with mixedcultures of shoot-inducing A. tumefaciens strains T37 and either Agrobacterium strain LBA1834(pRAL1834), (Hille et al. 1983) or LBA4404(pBIN6; pRAL4404), (Bevan 1984). Two small-scale feasibility experiments gave at least four ‘Maris Bard’ plants transformed with pRAL1834 T-DNA and two ‘Desiree’ plants with pBIN6 T-DNA. The transformed ‘Maris Bard’ plants were morphologically abnormal and highly aneuploid. This was probably an unfortunate side-effect of a tissue culture-step introduced to promote the efficiency of shoot regeneration. The transformed ‘Desiree’ plants, in contrast, were isolated without promoting additional shoot-growth. They were morphologically normal, contained 47 and the euploid 48 chromosomes per cell respectively and had improved growth on media containing kanamycin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00260785
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  • 8
    Electronic Resource
    Electronic Resource
    Transactivation of a target gene through feedforward loop activation in plants (2000)
    Springer
    Functional & integrative genomics 1 (2000), S. 35-43 
    Details
    ISSN: 1438-7948
    Keywords: Gene expression Plant Positive feedback Transcription Transcription factor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. The expression of many genes encoding transcriptional activators in prokaryotes and eukaryotes is upregulated through positive feedback activation. During positive feedback activation, a transcriptional activator binds to its own promoter and thus increases its own expression as well as the expression of its target genes. In the simplest case, increased levels of the transcriptional activator can be directly correlated with increased expression of its target genes. In this study, we present a gene expression system, designated feedforward loop (FFL) system, which makes use of this kind of positive feedback regulation for the expression of plant transgenes. We show in transient and stable transformation experiments that such a system is functional and that it can be used to obtain high level gene expression in plants. We also provide evidence that the transgene is ubiquitously expressed when using the FFL system. Finally, we discuss the possibilities of using FFL gene expression systems for applications in plant molecular genetics and biotechnology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s101420000001
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  • 9
    Electronic Resource
    Electronic Resource
    The tomato polygalacturonase gene and ripening-specific expression in transgenic plants (1988)
    Springer
    Plant molecular biology 11 (1988), S. 651-662 
    Details
    ISSN: 1573-5028
    Keywords: tomato fruit ripening ; polygalacturonase ; gene sequence ; transgenic tomato ; fruit-specific expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Polygalacturonase (PG) is the major cell wall degrading enzyme of tomato fruit. It is developmentally regulated and is synthesised de novo in ripening fruit. Genomic clones encoding a PG gene of tomato (Lycopersicon esculentum Mill cv. Ailsa Craig) have been isolated, mapped and sequenced. The sequence of the protein-coding region is identical to that of a PG cDNA [20]. Comparison of the cloned restriction fragments with genomic Southern data suggests that there may only be one gene for PG per haploid genome. The PG gene, which covers approximately 7 kb, is interrupted by 8 intervening sequences ranging in size from 99 bp to 953 bp. The transcription start point was identified by S1 mapping and primer extension analysis. About 1.4 kb of 5′ flanking DNA has been sequenced. This contains putative TATA and CAAT boxes and also direct repeat sequences. A transcriptional fusion has been constructed between the putative 1.4 kb promoter fragment and the chloramphenicol acetyl transferase (CAT) gene. Constructs containing this gene have been transferred to tomato using binary vectors. Regenerated transgenic plants express CAT in ripe tomato fruit, but not in unripe tomatoes, leaves, or roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00017465
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  • 10
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    A Shared Memory Module for Asynchronous Arrays of Processors (2007)
    Springer
    Details
    Publication Date: 2007-01-01
    Print ISSN: 1687-3955
    Electronic ISSN: 1687-3963
    Topics: Electrical Engineering, Measurement and Control Technology , Computer Science
    Published by Springer
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