Skip to main content
SpringerLink
Log in

A rapid transformation method for Solanum tuberosum using binary Agrobacterium tumefaciens vectors

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

A tuber disc transformation and regeneration system was devised for potato (Solanum tuberosum). Tuber discs were found to be the most morphogenetic organ on a medium previously optimised for tomato regeneration. Shoot regeneration from tuber discs was rapid and transformed as shown by nopaline assays and Southern blot analysis. The ease and speed of the tuber disc method will allow for the increased use of this commercially important plant in transformation studies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

MS:

Murashige and Skoog medium

IAA:

indole 3-acetic acid

BAP:

benzylaminopurine

NAA:

naphthalene acetic acid

ZR:

zeatin riboside

References

  • An, G., Watson, B.D., Chiang, C.C. (1986) Plant Physiol. 81:301–305.

    Google Scholar 

  • Bevan, M.W. (1984) Nucl. Acids Res. 12:8711–8721.

    Google Scholar 

  • De Cleene, M., De Ley, J. (1976) Botanical Review 42:389–466.

    Google Scholar 

  • Foulger, D., Jones, M.G.K. (1986) Plant Cell Reports 5:72–77.

    Google Scholar 

  • Gunn, R.E., Day, P.R. (1986) In: Plant Tissue Culture and its Biotechnological Applications. Butterworths, London, pp 313–316.

    Google Scholar 

  • Hangarter, R.P., Peterson, M.D., Good, N.E. (1980) Plant Physiol. 65:761–767.

    Google Scholar 

  • Herrera Estrella, L., De Block, M., Messens, E., Hernalsteens, J.P., Van Montagu, M., Schell, J. (1983) EMBO J. 2:987–995.

    Google Scholar 

  • Horsch, R.B., Fry, S.E., Hoffmann, N.L., Eicholtz, D., Rogers, S.G., Fraley, R.T. (1985) Science 277:1229–1231.

    Google Scholar 

  • Karp, A., Nelson, R.S., Thomas, E., Bright, S.W.J. (1982) Theor. Appl. Genet. 63:265–272.

    Google Scholar 

  • Maniatis, T., Fritsch, E.F., Sambrook, J. (1982) Molecular Cloning: A laboratory manual. Cold Spring Harbor Press, New York.

    Google Scholar 

  • Murashige, T., Skoog, F. (1962) Physiol. Plant. 15:473–497.

    Google Scholar 

  • Ooms, G., Hooykaas, P.J.J., Van Veen, R.J.M., Van Beelten, P., Regensburg, T.J.G., Schilperoort, R.A. (1982) Plasmid 7:15–29.

    Google Scholar 

  • Ooms, G., Burrell, M.M., Karp, A., Bevan, M., Hille, J. (1987) Theor. Appl. Genet. 73:744–750.

    Google Scholar 

  • Otten, L.A.B.M., Schilperoort, R.A. (1978) Biochim. Biophys. Acta 527:494:500.

    Google Scholar 

  • Shepard, J.F., Bidney, D., Shahin, E. (1980) Science 208:17–24.

    Google Scholar 

  • Tatchell, S., Binns, A.N. (1986) Tomato Genetics Co-op. Newsletter 36:35–36.

    Google Scholar 

  • Thomas, E., Bright, S.W.J., Franklin, J., Lancaster, V.A., Miflin, B.J., Gibson, R. (1982) Theor. Appl. Genet. 72:65–68.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Molecular Genetics Department, Institute of Plant Science Research (Cambridge Laboratory), Maris Lane, Trumpington, CB2 2LQ, Cambridge, UK

    S. Sheerman & M. W. Bevan

Authors
  1. S. Sheerman
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. W. Bevan
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by J. Schell

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sheerman, S., Bevan, M.W. A rapid transformation method for Solanum tuberosum using binary Agrobacterium tumefaciens vectors. Plant Cell Reports 7, 13–16 (1988). https://doi.org/10.1007/BF00272967

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00272967

Keywords

Search

Navigation