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Biotechnologies for Plant Mutation Breeding : Protocols (2017)

Jankowicz-Cieslak, Joanna ; Tai, Thomas H ; Kumlehn, Jochen ; [weitere]

Cham : Springer

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Schlagwort(e): Life sciences ; Genetic engineering ; Agriculture ; Plant genetics ; Plant breeding ; Life Sciences ; Plant Breeding/Biotechnology ; Agriculture ; Genetic Engineering ; Plant Genetics & Genomics

Beschreibung / Inhaltsverzeichnis: Mutagenesis for Crop Breeding and Functional Genomics --- Chemical and Physical Mutagenesis in Jatropha curcas --- Chemical Mutagenesis and Chimera Dissolution in Vegetatively Propagated Banana --- Mutation Induction Using Gamma Irradiation and Embryogenic Cell Suspensions in Plantain (Musa spp.) --- Optimization of Somatic Embryogenesis in Cassava --- Creation of a TILLING Population in Barley after Chemical Mutagenesis with Sodium Azide and MNU --- Site-Directed Mutagenesis in Barley by Expression of TALE Nuclease in Embryogenic Pollen --- Doubled Haploidy as a Tool for Chimera Dissolution of TALEN-Induced Mutations in Barley --- Field Evaluation of Mutagenized Rice Material --- Root Phenotyping Pipeline for Cereal Plants --- Breeding New Aromatic Rice with High Iron using Gamma Radiation and Hybridization --- Utilizing NIRS for Qualitative and Non-Destructive Identification of Seed Mutants in Large Populations --- Protocols for Proteome Analyses of Jatropha curcas --- Low-Cost Methods for DNA Extraction and Quantification --- A Protocol for Benchtop Extraction of Single-Strand-Specific Nucleases for Mutation Discovery --- A Protocol for Validation of Doubled Haploid Plants by Enzymatic Mismatch Cleavage --- Bioinformatics-Based Assessment of the Relevance of Candidate Genes for Mutation Discovery --- Mutation Detection by Analysis of DNA Heteroduplexes in TILLING Populations of Diploid Species --- Determining Mutation Density using Restriction Enzyme Sequence Comparative Analysis (RESCAN) --- Next-Generation Sequencing for Targeted Discovery of Rare Mutations in Rice

Seiten: Online-Ressource (XX, 340 pages) , 76 illustrations, 69 illustrations in color

ISBN: 9783319450216

URL: https://link.springer.com/openurl?genre=book&isbn=978-3-319-45021-6

Sprache: Englisch

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Digitale Medien

Stable haploid poplar callus lines from immature pollen culture (2004)

Deutsch, Frank ; Kumlehn, Jochen ; Ziegenhagen, Birgit ; [weitere]

Oxford, UK; Malden , USA : Munksgaard International Publishers

Physiologia plantarum 120 (2004), S. 0

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ISSN: 1399-3054

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Biologie

Notizen: Embryogenesis and plant regeneration have been obtained from isolated immature pollen of two poplar hybrids (Populus nigra L. × hybrid ‘Aue1’ and ‘Aue2’). In total, 1487 calli or embryos, respectively, larger than 1 mm were generated in a 2-year study. By using a cytokinin containing induction medium, on average 19 calli per responsive immature catkin were formed. Additional supplementation with auxin in 2002 increased the frequency to 72 calli per catkin. Microsatellite marker analyses confirmed haploid origin in most regenerants studied. So far six out of eight obtained regenerative callus lines have maintained their haploid level up to 24 months of development. A number of haploid and doubled haploid plants of different lines are available and have been transferred to soil.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1111/j.0031-9317.2004.0266.x

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