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  • 1
    Electronic Resource
    Electronic Resource
    ConcanavalinA receptors and the chemosensory behaviour of Tetrahymena thermophila (1997)
    Springer
    Experimental biology online 1 (1997), S. 1-12 
    Details
    ISSN: 1430-3418
    Keywords: Concanavalin A receptors ; Recognition ; Chemosensory behaviour ; Tetrahymena
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The relationship between concanavalin A (ConA) receptors and the chemosensory behaviour of the ciliated protozoan Tetrahymena thermophila was studied using the peptide chemoattractants proteose peptone and fibroblast growth factor. Studies on the chemosensory behaviour in semisolid methylcellulose showed that 50 μg/ml ConA selectively inhibited the persistent element of swimming behaviour by reducing time runs of cells responding to proteose peptone from 12.2±4.5 min to 0.8±0.3 min. Methyl-alpha-D-mannoside, but not methyl-alpha-D-galactoside, abolished the inhibitory effect of ConA, suggesting that mannoside-containing ConA receptors are involved in maintaining a persistent swimming behaviour. Control experiments, carried out in liquids where persistent swimming is less important for cellular behaviour, showed that ConA did not affect proteose-peptone-induced chemoattraction under these conditions as measured by a two-phase assay for chemoattraction. Also, no inhibitory effect of ConA could be found on swimming rates when individual velocities of ConA-treated cells were determined. When tested in liquid chemoattraction assays, ConA was found to be a weak but significant chemoattractant. Studies of the cellular location of ConA receptors on the plasma membrane of starved cells showed an unequal distribution. A preferential clustering of receptors at the anterior end of the cell was observed when determined at high concentrations (100 μg/ml) of fluorescent ConA. Methyl-alpha-D-mannoside but not methyl-alpha-D-galactoside abolished the fluorescent ConA labelling, indicating a preferential clustering of these mannoside-containing receptors at the anterior part of the plasma membrane and cilia. At lower concentrations (25 μg/ml), FITC-ConA produced more general labelling of the entire cell membrane. The results suggest that ConA receptors are necessary for the persistent element of swimming and that binding of ConA to its receptors interferes with processes related to signal transduction rather than by limiting the free movement of cilia required for locomotion. The gradient of receptors seen at high FITC-ConA concentrations may be important for a putative spatial chemosensory mechanism, i.e. chemotaxis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s00898-996-0005-0
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  • 2
    Electronic Resource
    Electronic Resource
    On the Role of Calcium in the Regulatory Volume Decrease (RVD) Response in Ehrlich Mouse Ascites Tumor Cells (1997)
    Springer
    The journal of membrane biology 157 (1997), S. 281 -299 
    Details
    ISSN: 1432-1424
    Keywords: Key words: Volume regulation — Phosphoinositidase C — Ins(1,4,5)P3— Ca2+signaling — K+ channels — Charybdotoxin — Clotrimazole
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract. The putative role for Ca2+ entry and Ca2+ mobilization in the activation of the regulatory volume decrease (RVD) response has been assessed in Ehrlich cells. Following hypotonic exposure (50% osmolarity) there is: (i) no increase in cellular Ins(1,4,5)P3 content, as measured in extracts from [2-3H]myoinositol-labeled cells, a finding at variance with earlier reports from our group; (ii) no evidence of Ca2+-signaling recorded in a suspension of fura-2-loaded cells; (iii) Ca2+-signaling in only about 6% of the single, fura-2-loaded cells at 1-mm Ca2+ (1% only at 0.1-mm Ca2+ and in Ca2+-free medium), as monitored by fluorescence-ratio imaging; (iv) no effect of removing external Ca2+ upon the volume-induced K+ loss; (v) no significant inhibition of the RVD response in cells loaded with the Ca2+ chelator BAPTA when the BAPTA-loading is performed in K+ equilibrium medium; (vi) an inhibition of the swelling-induced K+ loss (about 50%) at 1-mm Ba2+, but almost no effect of charybdotoxin (100 nm) or of clotrimazole (10 μm), reported inhibitors of the K+ loss induced by Ca2+-mobilizing agonists. Thus, Ca2+signaling by Ca2+ release or Ca2+ entry appears to play no role in the activation mechanism for the RVD response in Ehrlich cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002329900236
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of chronic oral furosemide administration on the 24-hour cycle of lithium clearance and electrolyte excretion in humans (1990)
    Springer
    European journal of clinical pharmacology 38 (1990), S. 275-280 
    Details
    ISSN: 1432-1041
    Keywords: Lithium clearance ; furosemide ; circadian variations ; sodium ; potassium ; creatinine clearance ; electrolyte excretion ; renal tubular physiology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary The effect of chronic furosemide treatment on the circadian cycle of lithium clearance (CLLi) and electrolyte excretion has been examined in 8 young, male volunteers, by performing two 24 h clearance experiments, before and after one week of treatment with furosemide 80 mg once daily. After 8 days on furosemide there was a significant decrease in creatinine clearance (−21%), plasma Na (−8.4 mM) and plasma K (−0.58 mM). At that time, however, there were no changes in 24 h-values of CLLi or Na excretion, although the magnitude of the circadian variation in CLLi and other renal parameters was increased. Both CLLi and CLNa were increased in the first 3 h following furosemide administration and thereafter they fell below the control level in the remaining hours of the experiment. From the absolute and fractional changes in CLLi it is suggested that compensatory Na conservation in response to chronic furosemide treatment occurs between doses, and that it involves decreased output from the proximal tubules combined with increased fractional Na reabsorption in the distal nephron.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315030
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  • 4
    Electronic Resource
    Electronic Resource
    Oxidation of atmospheric methane in Northern European soils, comparison with other ecosystems, and uncertainties in the global terrestrial sink (2000)
    Oxford, UK : Blackwell Science Ltd
    Global change biology 6 (2000), S. 0 
    Details
    ISSN: 1365-2486
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: This paper reports the range and statistical distribution of oxidation rates of atmospheric CH4 in soils found in Northern Europe in an international study, and compares them with published data for various other ecosystems. It reassesses the size, and the uncertainty in, the global terrestrial CH4 sink, and examines the effect of land-use change and other factors on the oxidation rate.Only soils with a very high water table were sources of CH4; all others were sinks. Oxidation rates varied from 1 to nearly 200 μg CH4 m−2 h−1; annual rates for sites measured for ≥1 y were 0.1–9.1 kg CH4 ha−1 y−1, with a log-normal distribution (log-mean ≈ 1.6 kg CH4 ha−1 y−1). Conversion of natural soils to agriculture reduced oxidation rates by two-thirds –- closely similar to results reported for other regions. N inputs also decreased oxidation rates. Full recovery of rates after these disturbances takes 〉 100 y. Soil bulk density, water content and gas diffusivity had major impacts on oxidation rates. Trends were similar to those derived from other published work. Increasing acidity reduced oxidation, partially but not wholly explained by poor diffusion through litter layers which did not themselves contribute to the oxidation. The effect of temperature was small, attributed to substrate limitation and low atmospheric concentration.Analysis of all available data for CH4 oxidation rates in situ showed similar log-normal distributions to those obtained for our results, with generally little difference between different natural ecosystems, or between short-and longer-term studies. The overall global terrestrial sink was estimated at 29 Tg CH4 y−1, close to the current IPCC assessment, but with a much wider uncertainty range (7 to 〉 100 Tg CH4 y−1). Little or no information is available for many major ecosystems; these should receive high priority in future research.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2486.2000.00356.x
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  • 5
    Electronic Resource
    Electronic Resource
    Sugarbeet yield response to competition from Sinapis arvensis or Lolium perenne growing at three different distances from the beet and removed at various times during early growth (2002)
    Oxford, UK : Blackwell Science Ltd
    Weed research 42 (2002), S. 0 
    Details
    ISSN: 1365-3180
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A sugarbeet field experiment was conducted in 1999 and 2000 to measure beet yield where Sinapis arvensis or Lolium perenne were growing in the crop row at 2, 4 or 8 cm from the beet plants. The weeds were removed by cutting once in the growing season in either late May, mid-June or early July. The number of neighbouring beet plants to every target beet plant was recorded. Projected leaf cover of a subset of the data with non-cut weeds was analysed by using image analysis to investigate whether this could be used to predict beet yield loss early in the growing season. Increasing the distance between beet and weed from 2 to 8 cm increased the beet yield significantly by an average of 20%, regardless of weed species. The dry weight of non-cut and re-growing weeds at harvest time decreased when cutting was postponed to the period between mid-June and early July. The number of neighbours described a sigmoidal yield decline of the single beet plants. Results from image analysis showed that approximately 33 g of beet yield was lost in October/November for each per cent relative projected leaf cover of the weeds in May, despite variation in growing conditions. The results are discussed in relation to potentials for robotic in-row weed control.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3180.2002.00301.x
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  • 6
    Electronic Resource
    Electronic Resource
    Cutting weeds with a CO2 laser (2001)
    Oxford UK : Blackwell Science Ltd
    Weed research 41 (2001), S. 0 
    Details
    ISSN: 1365-3180
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Stems of Chenopodium album. and Sinapis arvensis. and leaves of Lolium perenne. were cut with a CO2 laser or with a pair of scissors. Treatments were carried out on greenhouse-grown pot plants at three different growth stages and at two heights. Plant dry matter was measured 2 to 5 weeks after treatment. The relationship between dry weight and laser energy was analysed using a non-linear dose–response regression model. The regression parameters differed significantly between the weed species. At all growth stages and heights S. arvensis was more difficult to cut with a CO2 laser than C. album. When stems were cut below the meristems, 0.9 and 2.3 J mm−1 of CO2 laser energy dose was sufficient to reduce by 90% the biomass of C. album and S. arvensis respectively. Regrowth appeared when dicotyledonous plant stems were cut above meristems, indicating that it is important to cut close to the soil surface to obtain a significant effect. When cutting L. perenne plants with 2-true leaves at a height of 2 cm from the soil surface with a laser, the biomass decreased significantly compared with plants cut by scissors, indicating a delay in regrowth. This delay was not observed for the dicotyledonous plants nor for the other growth stages of L. perenne.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3180.2001.00212.x
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  • 7
    Electronic Resource
    Electronic Resource
    Using laser to measure stem thickness and cut weed stems (2002)
    Oxford, UK : Blackwell Science Ltd
    Weed research 42 (2002), S. 0 
    Details
    ISSN: 1365-3180
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Stem thickness of the weed Solanum nigrum and the crop sugarbeet was determined with a He–Ne laser using a novel non-destructive technique measuring stem shadow. Thereafter, the stems were cut close to the soil surface with a CO2 laser. Treatments were carried out on pot plants, grown in the greenhouse, at two different growth stages, and plant dry matter was measured 2–5 weeks after treatment. The relationship between plant dry weight and laser energy was analysed using two different non-linear dose–response regression models; one model included stem thickness as a variable, the other did not. A binary model was also tested. The non-linear model incorporating stem thickness described the data best, indicating that it would be possible to optimize laser cutting by measuring stem thickness before cutting. The general tendency was that more energy was needed the thicker the stem. Energy uses on a field scale are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3180.2002.00282.x
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  • 8
    Electronic Resource
    Electronic Resource
    Real-time weed detection, decision making and patch spraying in maize, sugarbeet, winter wheat and winter barley (2003)
    Oxford, UK : Blackwell Science Ltd
    Weed research 43 (2003), S. 0 
    Details
    ISSN: 1365-3180
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Information on temporal and spatial variation in weed seedling populations within agricultural fields is very important for weed population assessment and management. Most of all, it allows a potential reduction in herbicide use, when post-emergence herbicides are only applied to field sections with weed infestation levels higher than the economic weed threshold; a review of such work is provided. This paper presents a system for site-specific weed control in sugarbeet (Beta vulgaris L.), maize (Zea mays L.), winter wheat (Triticum aestivum L.) and winter barley (Hordeum vulgare L.), including online weed detection using digital image analysis, computer-based decision making and global positioning systems (GPS)-controlled patch spraying. In a 4-year study, herbicide use with this map-based approach was reduced in winter cereals by 60% for herbicides against broad-leaved weeds and 90% for grass weed herbicides. In sugarbeet and maize, average savings for grass weed herbicides were 78% in maize and 36% in sugarbeet. For herbicides against broad-leaved weeds, 11% were saved in maize and 41% in sugarbeet.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3180.2003.00349.x
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  • 9
    Electronic Resource
    Electronic Resource
    A decision algorithm for patch spraying (2003)
    Oxford, UK : Blackwell Science Ltd
    Weed research 43 (2003), S. 0 
    Details
    ISSN: 1365-3180
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: It has been established that weeds are spatially aggregated with a spatially varying composition of weed species within agricultural fields. Site-specific spraying therefore requires a decision method that includes the spatial variation of the weed composition and density. A computerized decision method that estimates an economic optimal herbicide dose according to site-specific weed composition and density is presented in this paper. The method was termed a ‘decision algorithm for patch spraying’ (DAPS) and was evaluated in a 5-year experiment, in Denmark. DAPS consists of a competition model, a herbicide dose–response model and an algorithm that estimates the economically optimal doses. The experiment was designed to compare herbicide treatments with DAPS recommendations and the Danish decision support system PC-Plant Protection. The results did not show any significant grain yield difference between DAPS and PC-Plant Protection; however, the recommended herbicide doses were significantly lower when using DAPS than PC-Plant Protection in all years. The main difference between the two decision models is that DAPS integrates crop–weed competition and estimates the net return as a continuous function of herbicide dose. The hypothesis tested is that the benefit of using lower herbicide doses recommended by DAPS would disappear after a few years because weed density will increase and thus require higher doses. However, the results of weed counting every year did not confirm this hypothesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3180.2003.00344.x
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  • 10
    Electronic Resource
    Electronic Resource
    On analysis and optimization in structural acoustics — Part I: Problem formulation and solution techniques (1998)
    Springer
    Structural and multidisciplinary optimization 16 (1998), S. 83-95 
    Details
    ISSN: 1615-1488
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract This paper is devoted to problems of structuralacoustic coupling with emphasis on analysis, design sensitivity analysis and optimization. The paper is divided into two parts, and it is the aim of Part I to (i) give a brief survey of recent developments in sensitivity analysis and sound emission and NVH (Noise, Vibration and Harshness) design of acoustically loaded structures, and (ii) discuss alternative objective functions and optimization formulations for structural acoustics. The aims of Part II are to (i) present consistent numerical techniques commonly used for treatment of coupled structural and acoustic dynamics, (ii) use the structural optimization tool ODESSY for solution of several coupled problems, and (iii) compare the numerical efficiency of alternative techniques and the relevance of selected objective functions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01202818
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