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  • Blackwell Publishing Ltd  (4)
  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 122 (2003), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Barley (Hordeum vulgare L.) is often grown on alkaline zinc (Zn)-deficient soils where reductions in yield and grain quality are frequently reported. Currently, the use of Zn-based fertilizer along with Zn-deficiency-tolerant genotypes is considered the most thorough approach for cropping the Zn-deficient soils; however, developing or breeding genotypes with higher Zn efficiency requires a good understanding of the inheritance of tolerance to Zn deficiency. This study was conducted to determine genetic control of this trait in barley. Two parental cultivars ('Skiff, moderately tolerant; and ‘Forrest’, sensitive), 185 F2 plants, and 48 F2-derived F3 families from this cross were screened to determine inheritance of tolerance to Zn deficiency using a visual score of deficiency symptoms. The segregation ratios observed indicated that greater tolerance to Zn deficiency in ‘Skiff compared with ‘Forrest’ at the seedling stage is controlled by a single gene with no dominance. The results also indicate that visual scores are useful for genetic analysis of tolerance to Zn deficiency.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 95 (1995), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: An understanding of the transport pathway used by Zn and Mn to enter developing grains may allow measures to increase the Zn and Mn content of wheat grain grown on Zn/Mn deficient soils. For this reason, transport of Zn and Mn into developing grains of wheat (Triticum aestivum L. cv. Aroona) was investigated. Detached ears (18–22 days post-anthesis) were cultured for 48 h in a solution containing 185 kBq of 65Zn and 185 kBq of 54Mn. Transport of 65Zn to the grain was unaffected by removal of glumes but was slightly reduced after the lemma was removed. Heat girdling the peduncle slightly reduced the amount of 65Zn transported to the grain, whilst heat girdling the rachilla reduced transport of 65Zn to the grain to a greater degree, suggesting phloem transport to the rachilla. The transport inhibitor CCCP (carbonyl cyanide m-chlorophenyl hydrazone) blocked 65Zn transport to grain but not to lemma and glumes. Removing glumes and lemma and heat girdling the peduncle did not affect transport of 54Mn, but transport was slightly affected by heat girdling the rachilla, indicating xylem transport. CCCP blocked transport of 54Mn into the grain but not to lemma and glumes. It was concluded that xylem-to-phloem transfer of Zn occurs in the rachis and to a lesser extent in peduncle and lemma. The results suggest that the lemma may be an important site for phloem loading when the concentration of Zn within the xylem is high. The data also suggest that Mn was predominantly translocated to the spikelets in the xylem, but that transport to the grain was dependent upon membrane transport before entering the grain. Phloem loading of Mn into the grain vascular system may have occurred at the site of xylem discontinuity in the floral axis.
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 97 (1996), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The hypothesis that Zn and Mn are transported within the grain in a similar manner to sucrose was investigated in the developing wheat grain. Detached ears were cultured in solution containing 65Zn, 54Mn and [14C]-sucrose for 10 to 120 min at 18–22 days post-anthesis. At different times the grain was cut transversely into 1-mm sections and the radioactivity in each section determined The embryo region was damaged in some grains to investigate the effect of reduced accumulation rate on the transport of 65Za, 54Mn and [14C]-sucrose to the embryo. The distribution of 65Zn. 54Mn and [14C]-sucrose between the endosperm cavity sap. endosperm, embryo and pericarp in grains labelled for 2.5 and 6 h at 18–22 days post-anthesis was also determined. [14C]-su-crose was initially high in the first, embryo-containing section of the grain but decreased progressively to the distal end of the grain. The amount of 65Zn along the longitudinal axis of the grain was distributed evenly in each 1-mm section, whilst 54Mn accumulated exponentially in the first proximal 1-mm section of the grain and was distributed evenly in the remaining sections. Damaging the embryo had no effect on 65Zn and 54Mn transport to the section containing the embryo. The pericarp contained almost all of the grain 65Za and 54Mn, with small amounts found in the embryo, endosperm and endosperm cavity sap. Increasing amounts of [14C]-sucrose were found in the endosperm as time progressed. The rate of accumulation of 65Zn, 54Mn and [14C]-sucrose was much higher in the embiyo than the endosperm: the difference between the embryo and endosperm was especially large for 65Zn and 54Mn. It is suggested that 65Zn and 54Mn are not transported within the grain in the same way as [14C]-sucrose. [14C]-sucrose moves laterally out of the vascular system of the crease into the endosperm cavity and is subsequently taken up and stored in the endosperm. In contrast, 65Zn and 54Mn appear to be retained within the vascular system of the crease and may be transported more slowly to grain parts such as the embryo and pericarp tissue.
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 121 (2002), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Manganese (Mn) deficiency is a major constraint of alkaline soils around the world, particularly for cultivation of durum wheat, which is more intolerant of low Mn levels than either common wheat or barley. Genetic variation for Mn efficiency exists in the current germplasm of durum wheat. Several restriction fragment length polymorphisms (RFLPs) previously shown to be linked to the Mel1 locus for Mn efficiency on chromosome 4HS of barley were tested on 88 selected F2 plants of the durum cross, ‘Stojocri 2’ (Mn efficient) בHazar’ (Mn inefficient). The Mel1-linked RFLP marker Xcdo583a was closely linked to the trait and explained over 42% of the total variation for Mn efficiency in the ‘Stojocri 2’/‘Hazar’ F2 progeny. This marker has the potential to provide a valuable tool for the marker-assisted selection of Mn-efficient durum progeny derived from crosses with ‘Stojocri 2’.
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