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  • 1
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    Asparagine metabolism and nitrogen distribution during protein degradation in sugar-starved maize root tips (1992)
    Springer
    Details
    Publication Date: 1992-10-01
    Print ISSN: 0032-0935
    Electronic ISSN: 1432-2048
    Topics: Biology
    Published by Springer
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  • 2
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    Larger adenylate energy charge and ATP/ADP ratios in aerenchymatous roots of Zea mays in anaerobic media as a consequence of improved internal oxygen transport (1985)
    Springer
    Details
    Publication Date: 1985-01-01
    Print ISSN: 0032-0935
    Electronic ISSN: 1432-2048
    Topics: Biology
    Published by Springer
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  • 3
    Electronic Resource
    Electronic Resource
    Developmental regulation of anoxic stress tolerance in maize (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 18 (1995), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Anoxia associated with flooding stress is detrimental to plant growth and productivity. When maize seedlings 2 to 7 d old were exposed to anoxic stress, 3-d-old seedlings were found to have much lower tolerance than 2-d-old seedlings. Ninety per cent of 2-d-old seedlings survived 72 h of anoxic stress compared with 0% of the 3-d-old seedlings. Since 2-d-old isolated root tips survived anoxic stress better than 3-d-old tips, the anoxic tolerance of 2-d-old seedlings was independent of the translocation of nutrient reserves from the endosperm to the root. The addition of glucose to the medium improved the anoxia tolerance of 2-d-old seedlings by 25% but had no effect on 3-d-old seedlings. Acclimation by pre-cxposure to 4% oxygen and pre-treatment with 100mmol m−1 abscisic acid (ABA) improved the anoxia tolerance of 3-d-old seedlings by 2- and 4-fold, respectively. However, acclimation and ABA treatment had no effect on 2-d-old seedlings. The results indicate that anoxia tolerance in maize is develop-mentally regulated. The mechanism of anoxia tolerance innate to 2-d-old seedlings was inducible in 3-d-old seedlings by acclimation or treatment with ABA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.1995.tb00604.x
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  • 4
    Electronic Resource
    Electronic Resource
    Biochemical changes induced by accelerated aging in sunflower seeds. II. RNA populations and protein synthesis (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 80 (1990), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Sunflower seeds (Helianthus annuus L. cv Rodeo) slowly lose their viability when subjected to accelerated aging (42°C and 100% relative humidity). After 12 h imbibition, in vivo protein labelling profiles show dramatic changes as a function of accelerated aging. Total and poly(A+)-RNA content of embryonic axes were similar regardless of the time spent in hot and wet conditions. However, the ability of mRNA to be translated in cell-free system was largely reduced in acceleratedly aged seeds. In vitro protein synthesis revealed the observed changes to be largely quantitative rather than qualitative, and to result mainly from post-transcriptional regulation. However, the induction of two proteins (15 and 43 kDa) subsequent to accelerated aging resulted from an increase in their corresponding mRNA, The accelerated aging treatment of sunflower seeds primarily induces quantitative changes in the translation of pre-existing mRNA, rather than the production of new mRNA species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1990.tb05684.x
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  • 5
    Electronic Resource
    Electronic Resource
    Metabolism of [14C]ss-glucose and [14C]-acetate by lettuce embryos during early germination (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 64 (1985), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The metabolism of [‘4C]-labelled glucose and acetate has been investigated during the early germination - before radicle emergence - of lettuce (Lactuca sativa L., cv. Val d'Orge) embryos. Similar amounts of radioactivity from both substrates were evolved as C., or incorporated into organic acids, amino acids and proteins. A large part of the [14C]-glucose was also incorporated into sucrose and polysaecharides, and a small part into the glycerol moiety of lipids. Acetate was massively incorporated into lipids, and only slightly into neutral compounds. These results show that both glucose and acetate can be utilized as respiratory substrates during early germination of lettuce embryos. Various biosynthetic pathways leading to amino acids, proteins, polysaecharides and lipids are active during this period.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1985.tb08534.x
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  • 6
    Electronic Resource
    Electronic Resource
    Induced pore formation in membranes of cortex cells in excised Sorghum roots: its effect on membrane potential and nucleotide leakage (1983)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 57 (1983), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Measurements of membrane potentials were used for screening various molecules capable of inducing pore formation in cellular membranes for their ability to penetrate into excised roots of Sorghum bicolor (L.) Moench.Poly-l-lysine (30 000 MW) and amphotericin B did not affect cortical cells even though they affected epidermal cells; the lysine polymer and the amphotericin B micelle were apparently too large to diffuse into the roots. Polymyxins and filipin depolarized the epidermal cells as well as the cells of the cortex. Depolarization of cortical cells by filipin was slower than that by polymixins.Leakage of adenylate nucleotides was measured to obtain information concerning the size of the pores induced by polymyxins. Incubation with 200 μg/ml polymyxin B caused leakage of ATP from root segments. Leaked nucleotides were found in the medium; however, with time they were dephosphorylated, presumably by cell wall phosphatases. In situ ATP formation was observed when root segments were incubated with polymyxin, ADP, Pi, succinate and glucose, confirming that the induced pores were larger than the size of a nucleotide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1983.tb00735.x
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  • 7
    Electronic Resource
    Electronic Resource
    Effect of oxygen concentration on the senescence and energy metabolism of cut carnation flowers (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 73 (1988), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The effect of O2 concentration on energy metabolism and senescence has been studied in cut flowers of Dianthus caryophyllus L. cv. Scania. As compared to the control (21% O2), 5% O2 delays flower senescence as well as decay of nucleotide level and AEC (adenylate energy charge). An atmosphere of 100% O2 accelerates senescence as well as the decrease of nucleotide level and AEC. While anoxia brings about a faster decrease of ATP and AEC than of total nucleotides, hyperoxia brings about a faster decrease in adenyl nucleotides than in ATP and AEC values. Petal oxygen uptake is over 90% of the maximal value under 4% O2 and saturates at 10% O2. The development of senescence is dicussed as a two phase process (first phase-progressive and second phase-catastrophic) triggered by the action of hyperoxia, first on the system for energy utilization and later on the system for energy production, the degradation of which seems to be linked with increase in membrane permeability and withering.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1988.tb00613.x
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  • 8
    Electronic Resource
    Electronic Resource
    Energy charge and emergence of the coleoptile and radicle at varying oxygen levels in Echinochloa crus-galli (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 62 (1984), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Energy charge, [(ATP + 1/2 ADP)/(ATP + ADP + AMP)], was measured in Echinochloa crus-galli (L.) Beauv. var. oryzicola seeds germinated in an aerobic or anaerobic environment. Within 24 h after the seeds were imbibed, total nucleotides increased and energy charge values went from 0.6 to at least 0.8 in both air and N2. Upon transfer from air to N2, the energy charge initially decreased, but subsequently (within 24 h) increased to at least 0.8. Total nucleotides also increased after an initial decline when transferred from air to N2. Emergence of the coleoptile and radicle were also examined at oxygen levels ranging from 21% to less than 0.005%. At all O2 levels, the time for 50% emergence of coleoptiles was less than for radice emergence. This was most evident when the O2 concentration was 1% or less. These data indicate that one of the adaptations that allows E. crus-galli var. oryzicola to germinate and grow under anaerobic conditions is its ability to maintain a high level of energy. In its natural environment, this would permit coleoptile growth and penetration of the shoot into a more aerobic zone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1984.tb00360.x
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  • 9
    Electronic Resource
    Electronic Resource
    Larger adenylate energy charge and ATP/ADP ratios in aerenchymatous roots of Zea mays in anaerobic media as a consequence of improved internal oxygen transport (1985)
    Springer
    Planta 165 (1985), S. 51-58 
    Details
    ISSN: 1432-2048
    Keywords: Adenine nucleotide ; Adenylate energy charge ; Acrenchyma ; Anaerobiosis ; Anoxia ; Gas space (root) ; Oxygen ; Root (O2 supply) ; Zea (root, oxygen)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Internal transport of O2 from the aerial tissues along the adventitious roots of intact maize plants was estimated by measuring the concentrations of adenine nucleotides in various zones along the root under an oxygen-free atmosphere. Young maize plants were grown in nutrient solution under conditions that either stimulated or prevented the formation of a lysigenous aerenchyma, and the roots (up to 210 mm long) were then exposed to an anaerobic (oxygen-free) nutrient solution. Aerenchymatous roots showed higher values than non-aerenchymatous ones for ATP content, adenylate energy charge and ATP/ADP ratios. We conclude that the lysigenous cortical gas spaces help maintain a high respiration rate in the tissues along the root, and in the apical zone, by improving internal transport of oxygen over distances of at least 210 mm. This contrasted sharply with the low energy status (poor O2 transport) in non-aerenchymatous roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392211
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  • 10
    Electronic Resource
    Electronic Resource
    Molecular cloning and characterization of six cDNAs expressed during glucose starvation in excised maize (Zea mays L.) root tips (1995)
    Springer
    Plant molecular biology 28 (1995), S. 473-485 
    Details
    ISSN: 1573-5028
    Keywords: carbon catabolite repression ; cDNA ; gene expression ; stress-induced genes ; glucose-starvation ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In order to isolate glucose-starvation-related cDNAs in maize (Zea mays L.) root tips, a cDNA library was constructed with poly(A)+ mRNA from 24 h starved root tips. After differential screening of the library, we isolated six different cDNAs (named pZSS2 and pZSS7) which were expressed during glucose starvation. Time course analysis revealed that maximum expression of five of these genes occurs 30 h after the onset of the starvation treatment. On the contrary, the expression of mRNAs corresponding to pZSS4 was maximal at an early stage of starvation and then dramatically decreased. The expression of this gene did not seem to be specific for glucose starvation. The pattern of induction of the genes corresponding to pZSS2, pZSS3, pZSS5, pZSS6 and pZSS7 revealed that non-metabolizable sugars such as L-glucose and mannitol induce mRNA transcription similarly to glucose starvation. When D-glucose or any other metabolizable sugar was supplied, the level of transcripts was reduced. Nucleotide sequence analyses of the six cDNAs allowed identification of five of them by comparison with sequence data bases. The protein encoded by clone pZSS2 is analogous to a wound-induced protein from barley. Clones pZSS4 to pZSS7 encode, respectively, a transmembrane protein, a cysteine protease, a metallothionein-like protein and a chymotrypsin/subtilisin-like protease inhibitor. Clone pZSS3 shares no significant homology with any known sequence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020395
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