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Asparagine metabolism and nitrogen distribution during protein degradation in sugar-starved maize root tips

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Abstract

Excised maize (Zea mays L.) root tips were used to monitor the effects of prolonged glucose starvation on nitrogen metabolism. Following root-tip excision, sugar content was rapidly exhausted, and protein content declined to 40 and 8% of its initial value after 96 and 192 h, respectively. During starvation the contents of free amino acids changed. Amino acids that belonged to the same “synthetic family” showed a similar pattern of changes, indicating that their content, during starvation, is controlled mainly at the level of their common biosynthetic steps. Asparagine, which is a good marker of protein and amino-acid degradation under stress conditions, accumulated considerably until 45 h of starvation and accounted for 50% of the nitrogen released by protein degradation at that time. After 45 h of starvation, nitrogen ceased to be stored in asparagine and was excreted from the cell, first as ammonia until 90–100 h and then, when starvation had become irreversible, as amino acids and aminated compounds. The study of asparagine metabolism and nitrogen-assimilation pathways throughout starvation showed that: (i) asparagine synthesis occurred via asparagine synthetase (EC 6.3.1.1) rather than asparagine aminotransferase (EC 2.6.1.14) or the β-cyanoalanine pathway, and asparagine degradation occurred via asparaginase (EC 3.5.1.1); and (ii) the enzymic activities related to nitrogen reduction and assimilation and amino-acid synthesis decreased continuously, whereas glutamate dehydrogenase (EC 1.4.1.2–4) activities increased during the reversible period of starvation. Considered together, metabolite analysis and enzymic-activity measurements showed that starvation may be divided into three phases: (i) the acclimation phase (0 to 30–35 h) in which the root tips adapt to transient sugar deprivation and partly store the nitrogen released by protein degradation, (ii) the survival phase (30–35 to 90–100 h) in which the root tips expel the nitrogen released by protein degradation and starvation may be reversed by sugar addition and (iii) the cell-disorganization phase (beyond 100 h) in which all metabolites and enzymic activities decrease and the root tips die.

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Abbreviations

AlaAT:

alanine aminotransferase

AspAT:

aspartate aminotransferase

AS:

asparagine synthetase

Asnase:

asparaginase

AsnAT:

asparagine aminotransferase

β-CS:

β-cyanoalanine synthase

GDH:

glutamate dehydrogenase

Glnase:

glutaminase

GOGAT:

glutamate synthase

GS:

glutamine synthetase

NiR:

nitrite reductase

NR:

nitrate reductase

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Authors and Affiliations

  1. INRA, Centre de Recherche de Bordeaux, Station de Physiologie Végétale, BP 81, F-33883, Villenave d'Ornon Cedex, France

    Renaud Brouquisse, Franck James, Alain Pradet & Philippe Raymond

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  1. Renaud Brouquisse
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  2. Franck James
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  3. Alain Pradet
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  4. Philippe Raymond
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Brouquisse, R., James, F., Pradet, A. et al. Asparagine metabolism and nitrogen distribution during protein degradation in sugar-starved maize root tips. Planta 188, 384–395 (1992). https://doi.org/10.1007/BF00192806

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