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  • 1
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Groups of European sea bass Dicentrarchus labrax L., broodstock were fed two pelleted, lipid-enriched diets [maize oil, low essential fatty acid (EFA) or fish oil, high EFA]. Those fed the high EFA diet for 12 months or only during vitellogenesis (September to February) with the low EFA diet fed for the remaining 6 months, showed improved egg quality and higher hatching rates when compared to two groups fed the high EFA diet during either previtel-logenesis (February to September) or the spawning period (February to April). The higher quality eggs contained higher levels of total n-3 fatty acids, including increased levels of both docosahexaenoic acid (DHA) and eicosapaentaenoic acid (EPA). High levels of triacylglycerol (TAG) were linked with the poorer quality eggs from those groups fed the low EFA diet during the period of vitellogenesis. The present work identifies vitellogenesis as the period in the maturation during which EFA are incorporated most effectively into the developing oocytes.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 791 (1996), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Microbiology 47 (1993), S. 139-166 
    ISSN: 0066-4227
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 56 (2000), S. 0 
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Fifteen tagged female sea bass Dicentrarchus labrax were sampled weekly from September to April and plasma vitellogenin (VTG), testosterone (T), 17β-estradiol (E2), and two potential maturation inducing steroids (MISs): 17,20β-dihydroxy-4-pregnen-3-one (17,20βP) and 17,20β,21-trihydroxy-4-pregnen-3-one (20βS) assayed. An oocyte sample was obtained via intraovarian cannulation at each sampling time from every female and the stage of development of the most advanced clutch of oocytes determined and related to VTG and hormone plasma levels for each female. The mean number of ovulations per female was 1·75+0·25 when those females that did not present ovulations were excluded and up to 4 ovulations detected in some females. The highest plasma levels of T (c. 6 ng ml-1) were observed during postvitellogenesis and the beginning of maturation while maximum plasma levels of E2 (〉5 ng ml-1) were obtained during late vitellogenesis. VTG plasma levels increased throughout vitellogenesis peaking (c. 2·5 mg ml-1) at postvittelogenesis. For the first time significant changes of plasma progestogens were detected in European sea bass during the sexual cycle. The highest plasma level of 17,20βP (c. 1·1 ng ml-1) was observed during postvitellogenesis while the highest level of 20βS (c. 1·4 ng ml-1) coincided with final maturation. These results suggest that 17,20βP and 20βS play a role in the early and final maturation, respectively, in the European sea bass.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Stimulation of transcription from the Pseudomonas TOL plasmid xylS gene promoter (Ps) and the upperpathway operon promoter (Pu) is dependent on the positive regulator protein XylR activated by an effector molecule such as 3-cholorotoluene, and on RpoN, an RNA polymerase sigma factor. Mutational analysis of the Ps and Pu promoters showed that upstream activator sequences located between -110 and -218bp upstream of the main transcription initiation point are required for regulated expression from these promoters. A search for homologous nucleotide sequences in the -110to -218bp region in Pu and Ps revealed conserved sequences that may act as putative recognition sequences for the XylR protein. Ps and Pu exhibit another well-conserved region at around 50 bp, which is homologous to corresponding sites in other RpoN-dependent promoters and may constitute a binding site for integration host factor (IHF).
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: TOL plasmid PWWO of Pseudomonas putida contains two operons that specify a pathway for the degradation of aromatic hydrocarbons. The ‘upper’ operon encodes enzymes for the oxidation of toluene to benzoate and xylenes to toluates, whereas the meta-cleavage operon specifies the further oxidation of benzoate and toluates. Transcription of the upper pathway operon (s positively regulated by the XylR protein, which is activated by toluene/xylenes and their alcohol catabolic products, in combination with the NtrA protein, a sigma factor. Expression of the meta-operon is positively controlled by the XylS protein which is activated by meta-pathway substrates, and is Independent of NtrA protein. Expression of the meta pathway is also Induced by toluene/xylene-activated XylR protein via a cascade regulatory system in which this protein in combination with NtrA protein stimulates transcription from the xylS gene promoter. Hyper-production of XylS protein in turn provokes high level expression of the meta-operon, which is independent of meta-pathway substrates. The two promoters, which are activated by the XylR and NtrA proteins, the upper pathway promoter and the xylS gene promoter, exhibit three regions of homology centred at –12(5′–TTGCTÃG–3′), –24(5′ -TGGCPuT –3) and –45(5′-TTAAATÃGPuPuGCGPuTc-3′), with respect to their principal transcription initiation points. The possible physiological significance of activated XylR-protein-induced expression of the meta-operon through amplification of XylS protein levels is considered.
    Type of Medium: Electronic Resource
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  • 7
    Publication Date: 2015-06-24
    Description: The family of anticancer complexes that include the transition metal copper known as Casiopeínas® shows promising results. Two of these complexes are currently in clinical trials. The interaction of these compounds with DNA has been observed experimentally and several hypotheses regarding the mechanism of action have been developed, and these include the generation of reactive oxygen species, phosphate hydrolysis and/or base-pair intercalation. To advance in the understanding on how these ligands interact with DNA, we present a molecular dynamics study of 21 Casiopeínas with a DNA dodecamer using 10 μs of simulation time for each compound. All the complexes were manually inserted into the minor groove as the starting point of the simulations. The binding energy of each complex and the observed representative type of interaction between the ligand and the DNA is reported. With this extended sampling time, we found that four of the compounds spontaneously flipped open a base pair and moved inside the resulting cavity and four compounds formed stacking interactions with the terminal base pairs. The complexes that formed the intercalation pocket led to more stable interactions.
    Print ISSN: 0305-1048
    Electronic ISSN: 1362-4962
    Topics: Biology
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  • 8
    Publication Date: 2015-01-20
    Description: Different marine and coastal activities have diverse economic, environmental, and socio-cultural objectives, which can lead to conflict when these multidimensional activities coincide spatially or temporally. This is sometimes driven by a lack of understanding or other users' needs and consequentially adequate planning and the utilization of a common language is essential. By using a transparent approach based on multi-criteria analysis, we characterize and establish priorities for future development/conservation for all users in the coastal area using six representative European Case Studies with different levels of complexity. Results varied according to location, but significantly it was found that stakeholders tended to favour ecological and social over economic objectives. This paper outlines the methodology employed, the results derived, and the potential for this approach to reduce conflict in coastal and marine waters.
    Print ISSN: 1054-3139
    Electronic ISSN: 1095-9289
    Topics: Biology , Geosciences , Physics
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  • 9
    Publication Date: 2016-02-20
    Description: Biological production in heterologous hosts is of interest for the production of the C4 alcohol (butanol) and other chemicals. However, some hurdles need to be overcome in order to achieve an economically viable process; these include avoiding the consumption of butanol and maintaining tolerance to this solvent during production. Pseudomonas putida is a potential host for solvent production; in order to further adapt P. putida to this role, we generated mini-Tn 5 mutant libraries in strain BIRD-1 that do not consume butanol. We analyzed the insertion site of the mini-Tn 5 in a mutant that was deficient in assimilation of butanol using arbitrary PCR followed by Sanger sequencing and found that the transposon was inserted in the malate synthase B gene. Here, we show that in a second round of mutagenesis a double mutant unable to take up butanol had an insertion in a gene coding for a multisensor hybrid histidine kinase. The genetic context of the histidine kinase sensor revealed the presence of a set of genes potentially involved in butanol assimilation; qRT-PCR analysis showed induction of this set of genes in the wild type and the malate synthase mutant but not in the double mutant.
    Keywords: Biotechnology & Synthetic Biology
    Print ISSN: 0378-1097
    Electronic ISSN: 1574-6968
    Topics: Biology
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  • 10
    Publication Date: 2012-08-22
    Description: The build-up of large magmatic complexes can proceed piecemeal over periods of several million years through sequences of complex processes of magma production, differentiation, assimilation, final crystallization and subsequent metasomatic modification. All these stages can produce or modify minerals used as geochronometers, such as zircon, monazite and titanite. The present study exemplifies such complex relationships, also demonstrating how a systematic approach with comprehensive sampling and careful high-resolution U–Pb analyses can yield a coherent picture of the entire magmatic process. The study was conducted on the Pavia pluton, an elongated Variscan intrusion in the Ossa–Morena Zone of Portugal. The geochronological data show that the Pavia pluton was emplaced by the amalgamation of multiple magma pulses into the crust, over a period of c . 11 Myr. An early event at ~340 Ma, revealed by xenocrystic zircon, preceded the magmatic activity at the exposed level of the pluton, but is recognized as the main magmatic event elsewhere in the Ossa–Morena Zone. A second event at 328 Ma formed tonalite, trondhjemite and granodiorite, and subordinate differentiates in the central domains of the pluton (units I and II). A third event at c . 324 Ma emplaced granodiorite in the flanking domains III–V and the contemporaneous and widespread two-mica granite in domain VI, together with late rhyodacite porphyries, microgranodiorites, aplite–pegmatite and pegmatite dikes. A fourth event at 319–317 Ma was characterized by the emplacement of some microgranites and pegmatite dikes. These two last magmatic events also had an effect on the previously emplaced rocks, causing local overgrowths and isotopic resetting of minerals. The occurrence of a fifth magmatic event at depth at 313 Ma is the inferred cause of the hydrothermal activity responsible for local zircon, monazite and titanite resorption and/or recrystallization and for some of the textures exhibited by the main rock-forming minerals. The magmatic episodes were interspersed with periods of quiescence; this cyclicity presumably reflects an external control by the transtensional tectonic regime of the Ossa–Morena Zone.
    Print ISSN: 0022-3530
    Electronic ISSN: 1460-2415
    Topics: Geosciences
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