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  • 1
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Most genes for resistance to barley leaf scald map either to the Rrs1 locus on the long arm of chromosome 3H, or the Rrs2 locus on the short arm of chromosome 7H. Other loci containing scald resistance genes have previously been identified using lines derived from wild barley, Hordeum vulgare ssp. spontaneum. A single dominant gene conditioning resistance to scald was identified in a third backcross (BC3F3) line derived from an Israeli accession of wild barley. The resistance gene is linked to three microsatellite markers that map to the long arm of chromosome 7H; the closest of these loci, HVM49, maps 11.5 cM from the resistance gene. As no other scald resistance genes have been mapped to this chromosome arm, it is considered to be a novel scald resistance locus. As the Acp2 isozyme locus is linked to this scald resistance locus, at 17.7 cM, Acp2 is assigned to chromosome 7H. Molecular markers linked to the novel scald resistance gene, designated Rrs15, can be used in breeding for scald resistance.
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  • 2
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Three transgenic sugarcane populations produced by Agrobacterium tumefaciens infection were analysed for the presence of genomic variability. Plants of the original cultivar, plants regenerated without transformation, as well as transformed and untransformed calli were used as control treatments. Amplified fragment length polymorphism (AFLP) of DNA extracted from leaves or calli assessed genomic profiling. The average DNA polymorphism within each population was determined by calculating the polymorphism index, while the extent of genomic dissimilarity among individual plants within transgenic populations was verified in unweighted pair group method using arithmetic averages dendrograms. The results showed that the production of transgenic sugarcane plants by A. tumefaciens infection is accompanied by limited but detectable genomic changes and that, on average, these occur at the same rate in plant populations carrying different transgenes. Main factors contributing to somaclonal variation in transgenic sugarcane plants have been verified by pre-existing DNA polymorphism into the donor genotype and in vitro culture steps during the transformation procedure. The relevant practical conclusion from this finding is that the AFLP analysis may be effectively used to identify individual transgenic plants with the least genomic deviation from the parental ones. The selected genotype would be conserved as cultivated sugarcane is asexually propagated.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Marine mammal science 21 (2005), S. 0 
    ISSN: 1748-7692
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Mark-recapture techniques are widely used to estimate the size of wildlife populations. However, in cetacean photo-identification studies, it is often impractical to sample across the entire range of the population. Consequently, negatively biased population estimates can result when large portions of a population are unavailable for photographic capture. To overcome this problem, we propose that individuals be sampled from a number of discrete sites located throughout the population's range. The recapture of individuals between sites can then be presented in a simple contingency table, where the cells refer to discrete categories formed by combinations of the study sites. We present a Bayesian framework for fitting a suite of log-linear models to these data, with each model representing a different hypothesis about dependence between sites. Modeling dependence facilitates the analysis of opportunistic photo-identification data from study sites located due to convenience rather than by design. Because inference about population size is sensitive to model choice, we use Bayesian Markov chain Monte Carlo approaches to estimate posterior model probabilities, and base inference on a model-averaged estimate of population size. We demonstrate this method in the analysis of photographic mark-recapture data for bottlenose dolphins from three coastal sites around NE Scotland.
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 248 (2005), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The bacterial stringent response is a pleiotrophic physiological response that is evoked when bacteria are subjected to nutrient stress and is mediated through the accumulation of hyperphosphorylated guanine nucleotides ((p)ppGpp) which are synthesized by the combined action of the relA and spoT gene products. The relA and spoT genes were cloned from Neisseria gonorrhoeae strain MS11 and various insertional and deletion mutants were constructed. Deletion of the gonococcal relA gene abrogated the production of (p)ppGpp when the organism was starved for the amino acid serine. Also, N. gonorrhoeaeΔrelA null mutants were impaired for growth when propagated on rich medium, a phenotype that could be relieved by deleting the spoT gene. Sequence analysis of the gonococcal SpoT polypeptide indicated a strong similarity to its Escherichia coli counterpart. However, in contrast to studies with E. coli, insertional spoT mutants could be obtained that still accumulated (p)ppGpp when gonococci were starved for nutrients provided that the non-polar insertions were located downstream of the putative phosphohydrolase active site. In time course studies, it is also shown that gonococci rapidly accumulate (p)ppGpp (within 5 min) when encountering nutrient deprivation.
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 70 (2005), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: North American ginseng (Panax quinquefolius) has a long history of use and is currently a commercially reliable natural health commodity. Ginsenosides or triterpene saponins are generally regarded as bioactive constituents for several observed health effects associated with ginseng. North American ginseng was dried using 3 different drying techniques to assess the ginsenoside content of prepared extracts. Drying methods included freeze-drying (FD), air-drying (AD), and vacuum microwave-drying (VMD) of ginseng root. High-performance liquid chromatography (HPLC) analysis showed that FD ginseng processing gave greater (P≥ 0.05) amounts of the fingerprint ginsenosides Rg1 (28 ± 0.9 mg/g, dry weight) and Re (45 ± 0.1) compared with AD (Rg1 19 ± 0.7, Re 29 ± 0.1) and VMD (Rg1 22 ± 0.8, Re 24 ± 0.1); whereas, VMD produced greater amounts of Rb1 (83 ± 0.1) and Rd (13 ± 0.0) than FD (Rb1 62 ± 0.1, Rd 9 ± 0.1) and AD (Rb1 69 ± 0.1, Rd 5 ± 0.0), respectively. Total ginsenoside content was similar for FD and VMD and was the lowest (P≥ 0.05) for AD. Electrospray mass spectrometry (ESI-MS) analysis showed a total of 12 compounds detected in FD ginseng compared with 10 compounds in ginseng dried by both VMD and AD. Our results support the fact that FD and VMD drying methods of North American ginseng can improve both extraction efficiency and actual retention of individual ginsenoside in root material.
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    International journal of selection and assessment 13 (2005), S. 0 
    ISSN: 1468-2389
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Economics
    Notes: In response to Schmidt, Le, and Ilies' (2003) call for further assessment of transient error variance in measures of key individual difference variables, the current study examines transient error estimates for eight speeded cognitive ability scales and a composite scale. One hundred twenty-three participants were tested on two occasions with a 1-week interval between administrations. Estimates of transient error ranged from a low of .01 to a high of .18, with an average value of .09. The coefficient of equivalence, based on the split-half method, resulted in overestimates of reliability by as little as 1.90% to as much as 25.96%, with an average 12.96%.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    International journal of selection and assessment 13 (2005), S. 0 
    ISSN: 1468-2389
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Economics
    Notes: Standardized tests are commonly used to select individuals in both pre-employment and educational settings. Nevertheless, research has yet to sufficiently explore the relationship between test anxiety (TA) and test performance in selection contexts. The goal of this study was to assess the dimensionality of TA, the relations between TA and test performance, and the impact of gender on TA within the realm of personnel selection. Test anxiety was found to be bi-dimensional and negatively related to performance on a cognitively based selection instrument. Gender moderated the relation between TA and test performance, with stronger associations for males. This finding is consistent with the sex-linked anxiety coping theory that is advanced here. Implications of this study and avenues for future research are discussed.
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    International journal of selection and assessment 13 (2005), S. 0 
    ISSN: 1468-2389
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Economics
    Notes: A situational judgment test (SJT) and a Big 5 personality test were administered to 203 participants under instructions to respond honestly and to fake good using a within-subjects design. Participants indicated both the best and worst response (i.e., Knowledge) and the most likely and least likely response (i.e., Behavioral Tendency) to each situation. Faking effect size for the SJT Behavioral Tendency response format was (d=.34) when participants responded first under honest instructions and (d=.15) when they responded first under faking instructions. Those for the Big 5 dimensions ranged from d=.26 to d=1.0. For the Knowledge response format results were inconsistent. Honest condition Knowledge SJT scores were more highly correlated with cognitive ability (r=.56) than were Behavioral Tendency SJT scores (r=.38). Implications for researchers and practitioners are discussed.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 21 (2005), S. 0 
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. Over the last three decades there has been a great increase in the production of waste from urban, industrial and agricultural activity that could be recycled as a source of plant nutrients, and used to enhance soil quality. The use of these materials could partially offset the need for mineral fertilizers, giving both economic and environmental benefits. An incubation experiment was carried out using different organic waste materials applied to a Cambic Arenosol. Air-dried soil was mixed with increasing amounts of composted solid municipal waste, secondary pulp-mill sludge, hornmeal, poultry manure, solid phase from pig slurry, and composted pig manure, resulting in applications equivalent to 0, 40, 80, 120, 160 and 200 kg ha−1 of Kjeldahl nitrogen. The samples were incubated for 244 days under a controlled environment of 24 °C and 60% water holding capacity of the soil. The increasing amounts of waste applied always led to a greater amount of potentially available nitrogen present in the soil/waste mixture. Based on the proportion of their active N fraction, wastes were ranked: poultry manure〉hornmeal〉solid phase from pig slurry〉composted pig manure〉secondary pulp-mill sludge〉composted municipal solid waste. The results were well described by a one-pool exponential mineralization model, and mineral N formation was proportional to the quantity of waste applied. Of the wastes tested, those from animal sources showed greater nitrogen mineralisation. Nitrification was rapid, and concentrations of ammonium nitrogen remained relatively small.
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  • 10
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This study tests relationships between, on the one hand, field-saturated infiltration rate into the uppermost sloping soil layer (Kfsi and field-saturated hydraulic conductivity in soil horizons (Kfsh) and, on the other hand, in situ determined macropore sizes in soil profile descriptions. It was carried out at six locations along a transect on a slope with loamy soils. The macropores were classified into representative size indices based on pore diameters (Ptd) and pore areas (Pta). A strong relationship was found between Kfsi and Pta, and between Kfsh and Ptd. The approach is promising and the methodology could be further developed with the aim of generalizing the functional relationships, so that Kfs can be estimated in areas where soil survey databases contain information on pore size abundance but lack Kfs values.
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