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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 47 (2004), S. 0 
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Most widely used medium for cultivation of methanotrophic bacteria from various environments is that proposed in 1970 by Whittenbury. In order to adapt and optimize medium for culturing of methanotrophs from freshwater sediment, media with varying concentrations of substrates, phosphate, nitrate, and other mineral salts were used to enumerate methanotrophs by the most probable number method. High concentrations (〉1 mM) of magnesium and sulfate, and high concentrations of nitrate (〉500 μM) significantly reduced the number of cultured methanotrophs, whereas phosphate in the range of 15–1500 μM had no influence. Also oxygen and carbon dioxide influenced the culturing efficiency, with an optimal mixing ratio of 17% O2 and 3% CO2; the mixing ratio of methane (6–32%) had no effect. A clone library of pmoA genes amplified by PCR from DNA extracted from sediment revealed the presence of both type I and type II methanotrophs. Nonetheless, the cultivation of methanotrophs, also with the improved medium, clearly favored growth of type II methanotrophs of the Methylosinus/Methylocystis group. Although significantly more methanotrophs could be cultured with the modified medium, their diversity did not mirror the diversity of methanotrophs in the sediment sample detected by molecular biology method.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2021-10-20
    Description: MOSES (Modular Observation Solutions for Earth Systems) is a novel observation system that is specifically designed to unravel the impact of distinct, dynamic events on the long-term development of environmental systems. Hydro-meteorological extremes such as the recent European droughts or the floods of 2013 caused severe and lasting environmental damage. Modelling studies suggest that abrupt permafrost thaw events accelerate Arctic greenhouse gas emissions. Short-lived ocean eddies seem to comprise a significant share of the marine carbon uptake or release. Although there is increasing evidence that such dynamic events bear the potential for major environmental impacts, our knowledge on the processes they trigger is still very limited. MOSES aims at capturing such events, from their formation to their end, with high spatial and temporal resolution. As such, the observation system extends and complements existing national and international observation networks, which are mostly designed for long-term monitoring.Several German Helmholtz Association centers have developed this research facility as a mobile and modular “system of systems” to record energy, water, greenhouse gas and nutrient cycles on the land surface, in coastal regions, in the ocean, in polar regions, and in the atmosphere – but especially the interactions between the Earth compartments. During the implementation period (2017-2021), the measuring systems were put into operation and test campaigns were performed to establish event-driven campaign routines. With MOSES’ regular operation starting in 2022, the observation system will then be ready for cross-compartment and cross-discipline research on the environmental impacts of dynamic events.
    Print ISSN: 0003-0007
    Electronic ISSN: 1520-0477
    Topics: Geography , Physics
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