Publikationsdatum:
2002-07-06
Beschreibung:
Blue light regulates many physiological processes in fungi, but their photoreceptors are not known. In Neurospora crassa, all light responses depend on the Per-Arnt-Sim (PAS) domain-containing transcription factor white collar-1 (wc-1). By removing the WC-1 light, oxygen, or voltage domain, a specialized PAS domain that binds flavin mononucleotide in plant phototropins, we show that light responses are abolished, including light entrainment of the circadian clock. However, the WC-1-mediated dark activation of frq remains normal in this mutant, and the circadian clock can be entrained by temperature. Furthermore, we demonstrate that the purified Neurospora WC-1-WC-2 protein complex is associated with stoichiometric amounts of the chromophore flavin-adenine dinucleotide. Together, these observations suggest that WC-1 is the blue-light photoreceptor for the circadian clock and other light responses in Neurospora.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉He, Qiyang -- Cheng, Ping -- Yang, Yuhong -- Wang, Lixing -- Gardner, Kevin H -- Liu, Yi -- New York, N.Y. -- Science. 2002 Aug 2;297(5582):840-3. Epub 2002 Jul 4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Physiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12098705" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
Amino Acid Sequence
;
Circadian Rhythm/radiation effects
;
Color
;
DNA, Fungal/genetics/metabolism
;
DNA-Binding Proteins/chemistry/deficiency/genetics/*metabolism
;
Darkness
;
Dimerization
;
Flavin Mononucleotide/metabolism
;
Flavin-Adenine Dinucleotide/metabolism
;
Fungal Proteins/chemistry/genetics/metabolism
;
Gene Deletion
;
Gene Expression Regulation, Fungal/radiation effects
;
*Light
;
Molecular Sequence Data
;
Neurospora crassa/genetics/*metabolism/radiation effects
;
Oxygen/metabolism
;
Photoreceptors, Microbial/chemistry/genetics/*metabolism
;
Promoter Regions, Genetic/genetics
;
Protein Binding
;
Protein Structure, Tertiary
;
Response Elements/genetics
;
Temperature
;
Transcription Factors/chemistry/deficiency/genetics/*metabolism
Print ISSN:
0036-8075
Digitale ISSN:
1095-9203
Thema:
Biologie
,
Chemie und Pharmazie
,
Informatik
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
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