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  • 2020-2021
  • 1995-1999  (79)
  • 1965-1969  (2)
  • 1945-1949  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 45 (1998), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Degenerate primers for two regions of sequence homology in the myosin head domain were used in a polymerase chain reaction screen of Tetrahymena thermophila genomic DNA to amplify a 765 bp fragment that was cloned and sequenced. Based on the presence of conserved, myosin-specific sequences, the 765 bp PCR product was identified as a fragment of a myosin gene, the first to be discovered in ciliated protozoa and herein referred to as MYO1. An inverse polymerase chain reaction strategy was used to obtain additional sequence data that included the entire head domain of MYO1. Alignment of the predicted amino acid sequence of the MYO1 head domain with known myosin sequences identified the ATP-binding site, a phosphorylation site, and other myosin-specific consensus regions. In a northern blot analysis, a 765 bp MYO1-specific probe detected a 6.6 kb transcript under highly stringent hybridization conditions. Phylogenetic analysis revealed that the predicted protein encoded by MYO1 is not a member of any of the previously defined myosin classes and therefore represents a presumptive new myosin class.
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  • 2
    Electronic Resource
    Electronic Resource
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 75 (1999), S. 1503-1505 
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The intrinsic "self-trapped" hole center in KD2PO4 crystals has been identified using electron paramagnetic resonance and electron-nuclear double resonance. These defects, labeled [D2PO4]o centers, can be formed at 77 K by irradiating with either 60 kV x rays or the fourth harmonic (266 nm) of a pulsed Nd:YAG laser. The hole is equally shared by two adjacent oxygen ions, and hyperfine interactions with one phosphorus and two equivalent deuterons are observed. The sample used in this investigation was approximately 80% deuterated, thus both [D2PO4]o and [HDPO4]o centers were detected, with the former being dominant. These intrinsic self-trapped hole centers are of interest because of their potential role in the transient optical absorption produced in KD2PO4 crystals at room temperature by intense 266 nm laser pulses. © 1999 American Institute of Physics.
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  • 3
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Biochemistry 8 (1969), S. 633-640 
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 111 (1999), S. 2818-2828 
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: This paper presents an approach based on the saddle-point approximation to study the equilibrium interactions between small molecules and macromolecules with a large number of sites. For this case, the application of the Darwin–Fowler method results in very simple expressions for the stoichiometric equilibrium constants and their corresponding free energies in terms of integrals of the binding curve plus a correction term which depends on the first derivatives of the binding curve in the points corresponding to an integer value of the mean occupation number. These expressions are simplified when the number of sites tends to infinity, providing an interpretation of the binding curve in terms of the stoichiometric stability constants. The formalism presented is applied to some simple complexation models, obtaining good values for the free energies involved. When heterogeneous complexation is assumed, simple expressions are obtained to relate the macroscopic description of the binding, given by the stoichiomeric constants, with the microscopic description in terms of the intrinsic stability constants or the affinity spectrum. © 1999 American Institute of Physics.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Wireless networks 5 (1999), S. 95-109 
    ISSN: 1572-8196
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Computer Science
    Notes: Abstract We introduce a stable multiple access protocol for broadcast channels shared by bursty stations, which we call CARMA-NTQ (for collision avoidance and resolution multiple access with non-persistence and transmission queues). Like previous efficient MAC protocols based on tree-splitting algorithms (e.g., DQRAP), CARMA-NTQ maintains a distributed queue for the transmission of data packets and a stack for the transmission of control packets used in collision resolution. However, CARMA-NTQ does not require the mini-slots commonly used in protocols based on collision resolution. CARMA-NTQ dynamically divides the channel into cycles of variable length; each cycle consists of a contention period and a queue-transmission period. The queue-transmission period is a variable-length train of packets, which are transmitted by stations that have been added to the distributed transmission queue by successfully completing a collision-resolution round in a previous contention period. During the contention period, stations with packets to send compete for the right to be added to the data-transmission queue using a deterministic first-success tree-splitting algorithm, so that a new station is added to the transmission queue. A lower bound is derived for the average throughput achieved with CARMA-NTQ as a function of the size of the transmission queue and the number of queue-addition requests that need to be resolved. This bound is based on the upper bound on the average number of collision resolution steps needed to resolve a given number of queue-add requests.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 60 (1997), S. 391 -393 
    ISSN: 1432-0827
    Keywords: Key words: Bone growth — Fractures — Osteotomy.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract. One hundred and five 5-week-old male rats were used to study the influence of the stability of an osteotomy on longitudinal overgrowth of the bone. In 45 rats (Group 1) a transverse diaphyseal osteotomy of the right tibia was made; the left tibia was left untreated. In the second 45 rats (Group 2) the right tibia was osteotomized after applying an external fixator; the left tibia underwent a sham operation without osteotomy. Fifteen further rats (Group 3) were used as a nonoperated control group. After being measured at different periods up to 20 weeks, the osteotomized tibiae of the Group 1 (unstable) were 16–25% longer than those of their nontreated limbs (P 〈 0.001). The osteotomized tibiae of the Group 2 (stable) were 6–11% longer than those of the sham-operated opposite limbs (P 〈 0.001). The osteotomized and unstable tibiae were 9–17% longer than the osteotomized and stable tibiae (P 〈 0.001) throughout the whole study. Although several factors have been considered to be responsible for longitudinal bone overgrowth after fracture in young animals, this work suggests that bone's stability plays a decisive role in the final outcome.
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  • 7
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 192-194 (Aug. 1995), p. 907-0 
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
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  • 8
    ISSN: 1573-0417
    Keywords: Atacama ; Holocene ; limnogeology ; South America ; paleoclimatology ; lake sediments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract Sedimentological, mineralogical and geochemical analyses of sediment cores from 9 m-deep, saline Laguna Miscanti, Chile (23 ° 44′S, 67 °46′W, 4140 m a.s.l.) together with high-resolution seismic profiles provide a mid to late Holocene time series of regional environmental change in the Atacama Altiplano constrained by 210Pb and conventional 14C dating. The mid Holocene was the most arid interval since the last glacial maximum, as documented by subaerial exposure and formation of hardgrounds on a playa surface. Extremely low lake levels during the mid Holocene appear consistent with lower effective moisture recorded at other sites along the Altiplano and in the Amazon Basin. Termination of this arid period represented a major shift in the regional environmental dynamics and inaugurated modern atmospheric conditions. The cores show a progressive upward increase in effective moisture interrupted by numerous century-scale drier periods of various intensities and durations that characterize a fluctuating late Holocene climate. In spite of chronological uncertainties, the major environmental changes seem to correlate with the available paleorecords from the region providing a coherent account of effective moisture variability in the tropical highlands of South America.
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  • 9
    ISSN: 1573-0417
    Keywords: sedimentology ; saline lake ; meromixis ; Northern Great Plains ; Holocene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract Medicine lake is a small (about 1 km2), shallow (up to 10 m deep), saline (50–170 g l−1) and meromictic lake formed after the retreat of the Wisconsin ice in the north American Great Plains. Based on a detailed sedimentological analysis of cores, we describe and interpret 13 sedimentary subfacies grouped in 9 associations which characterize the following lacustrine subenvironments: clastic littoral (freshwater and saline), springs, microbial mats, bench slope, and pelagial (oxic, alternating oxic-anoxic, anoxic and hypersaline, and organic-dominated). Lateral distribution and vertical evolution of subfacies in our model are controlled by climate fluctuations, climate-related limnological parameters (lake level, TDS and brine composition, and redox conditions), and autocyclic processes (progressive infilling of the basin and higher sedimentation rate in the pclagial realm). Microbial and chemical processes govern deposition in this system, and meromixis plays a decisive role in lake dynamics. Phototropic bacterial plate communites at the chemocline dominated as pelagial organic producers during stable meromictic periods, whereas benthic microbial communities developed during mixed water periods. Water stratification during the Holocene was mainly controlled by three parameters: 1) basin morphometry, 2) lake level, and 3) differences in TDS values between mixolimnion and monimolimnion waters. Sedimentary facies analyses is a powerful descriptive and interpretative tool that greatly contributes to deciphering the high resolution paleoenvironmental information archived in lake sequences. Depositional and paleoenvironmental models provide a dynamic framework for integrating paleolimnological data and other proxy paleorecords. Medicine lake serves as a facies model for shallow, perennial hypersaline, meromictic lakes in modern and ancient lacustrine basins. The sediment sequence from Medicine lake cores is consistent with the general paleoclimatic evolution of the northern Great Plains since the retreat of ice sheets. Our study reveals a plethora of rapid fluctuations in the water cycle both during the middle and the late Holocene. These augment prior paleoclimate reconstructions based on diatom studies of the lower Holocene freshwater to saline transition and on pollen profiles which show little variability during the subsequent long prairie grass episode.
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  • 10
    ISSN: 1432-0827
    Keywords: Key words: Human osteoblastic cells — Skeletal site — Aging.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract. Bone loss with aging may be due, at least in part, to inadequate bone formation. Moreover, the process of bone aging is known to follow a different pattern throughout the skeleton. In this study, we examined the cell proliferation rate (area under the cell growth curve, AUC) and the secretion of C-terminal type I procollagen (PICP), alkaline phosphatase (ALP), and osteocalcin (OC) in primary cultures of osteoblastic cells from human trabecular bone. Osteoblastic cells were obtained for 168 donors (100 women and 68 men). Ninety-eight bone samples were obtained from subjects undergoing knee arthroplastia, 52 aged 50–70 years (64 ± 5) and 46 over age 70 (73 ± 2). Another 70 bone samples were obtained from subjects undergoing hip arthroplastia; 51 were 50–70 years old (64 ± 4) and 19 were over 70 (75 ± 5). Osteoblastic cells from the older donors had a lower proliferation rate and OC secretion than those from younger subjects. However, ALP secretion was higher in the former subjects, whereas PICP secretion was unchanged. Osteoblastic cells from hip had a lower proliferation rate than those from knee. PICP secretion was also lower and ALP secretion was higher in the former cells. In age-matched cell cultures, osteoblastic cells from the knee had higher proliferation rate and PICP secretion than osteoblastic cells from the hip. However, ALP secretion was lower in knee osteoblastic cells than those from hip only in the younger group. With aging, ALP secretion was found to increase in knee osteoblactic cells, whereas OC secretion decreased in osteoblastic cell cultures from the hip. Our findings suggest that bone loss with aging may be accounted for, at least in part, by a decreased osteoblastic cell proliferation and an increased osteoblastic maturation. In addition, our data indicate that these changes with aging do not occur similarly at different skeletal sites.
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