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  • 1970-1974  (3)
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  • 1
    Unknown
    Amsterdam ; New York : North-Holland
    Pages: Online-Ressource (x, 274 pages)
    ISBN: 9780444103574
    Language: English
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 81 (1974), S. 363-372 
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A new method is proposed for the isolation of tobacco mesophyll protoplasts in which salts were used as the plasmolyticum instead of the traditionally applied sugars. With this method protoplasts were regularly obtained and practically independent of the physiological state of the plants. In addition, a salt medium was developed in which the majority of protoplasts dedifferenciate and divide at a developmental state in which no wall could be detected by plasmolysis. After transferring these cells into another medium they were able to regenerate a wall and to divide.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 124 (1973), S. 167-176 
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The karyotypes of females and males ofSphaerocarpos donnellii differ in that there is a large essentially heterochromatic X chromosome in the female (approx. 25 volume-% of the autosomes) and a small Y chromosome in the male (0.1–3 volume-% of the autosomes). DNA from females and males differ in buoyant densities in cesium chloride equilibrium gradients (1.7025 and 1.7035g cm-3, respectively) and in melting points (87.5 and 88.5°C in SSC). The differences are statistically significant. Base analyses revealed 2.5 Mol-% of the rare base 5-methyl cytosine. Upon reassociationSphaerocarpos DNA behaves kinetically in a heterogeneous manner. About 22% of the DNA is repetitive with an average kinetic complexity of 1.6×108 daltons. The kinetic complexity of the slow reassociating DNA fraction, considered to be of the single copy type, is 3.2×1010 daltons. No difference in the renaturation behavior between DNA of males and females could be detected with the techniques used. Our data thus indicate that X chromosomal DNA cannot contain large amounts of highly repeated nucleotide sequences and that it is slightly enriched in AT content compared to the autosomes.
    Type of Medium: Electronic Resource
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