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  • 1975-1979  (3)
Collection
Publisher
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 152 (1977), S. 319-324 
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In the antimycin-resistant mutantana r-8 of the fission yeastSchizosaccharomyces pombe (Sch.p.) spontaneous mutants were isolated showing high resistance to the aminoglycoside antibiotic paromomycin. All mutants were resistant to the structurally related antibiotic neomycin. Tetrad analysis, mitotic segregation analysis, and mitotic haploidization revealed extrachromosomal, very likely mitochondrial inheritance. In contrast to the rapid segregation of mitochondrial markers in zygotic clones ofSaccharomyces cerevisiae (S.c.) the heteroplasmic state of diploids proved to persist for at least 50 generations after zygote formation. Stationary cultures of the paromomycin-resistant mutantspar r-106 andpar r-112 contain up to 6% respiratory-deficient mutants, but no reversion to paromomycin-sensitivity was observed among 1700–1800 colonies tested. The ability of mutantana r-8 to produce spontaneously respiratory-deficient mutants could be separated from the antimycin-resistant phenotype ofana r-8.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 155 (1977), S. 339-346 
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Spontaneous chloramphenicol (cap r)- and erythromycin (ery r)-resistant mutants were isolated from strain ade7–50 h - and the antimycin-resistant mutant ana r-8 ade 7–50 h- of Schizosaccharomyces pombe (Sch. p.). By mitotic segregation analysis all 154 cap r- and 120 ery r-mutants derived from ade 7–50 h - proved to be recessive chromosomal, whereas all 108 cap r- and 200 ery r-mutants originating from ana r-8 were extrachromosomally inherited. The rate of spontaneous cap r- and ery r-mutants was about hundredfold in ana r-8 compared to ade 7–50 h -. Growth of cap r-and ery r-mutants was not inhibited by chloramphenicol or erythromycin, respectively, in glucose-medium and only slightly in glycerol-medium at concentrations which completely inhibited ana r-8. By mitotic segregation-, tetrad-, and mitotic haploidization-analysis the extrachromosomal inheritance of mutants derived from ana r-8 was established. Segregational patterns of cap r- and ery r-determinats during mitosis, meiosis, and mitotic haplidization of diploids are discussed.
    Type of Medium: Electronic Resource
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  • 3
    Publication Date: 1978-04-01
    Print ISSN: 0009-2614
    Electronic ISSN: 1873-4448
    Topics: Chemistry and Pharmacology , Physics
    Published by Elsevier
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