Summary
In the antimycin-resistant mutantana r-8 of the fission yeastSchizosaccharomyces pombe (Sch.p.) spontaneous mutants were isolated showing high resistance to the aminoglycoside antibiotic paromomycin. All mutants were resistant to the structurally related antibiotic neomycin. Tetrad analysis, mitotic segregation analysis, and mitotic haploidization revealed extrachromosomal, very likely mitochondrial inheritance. In contrast to the rapid segregation of mitochondrial markers in zygotic clones ofSaccharomyces cerevisiae (S.c.) the heteroplasmic state of diploids proved to persist for at least 50 generations after zygote formation. Stationary cultures of the paromomycin-resistant mutantspar r-106 andpar r-112 contain up to 6% respiratory-deficient mutants, but no reversion to paromomycin-sensitivity was observed among 1700–1800 colonies tested. The ability of mutantana r-8 to produce spontaneously respiratory-deficient mutants could be separated from the antimycin-resistant phenotype ofana r-8.
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Del Giudice, L., Wolf, K., Seitz, G. et al. Extrachromosomal inheritance inSchizosaccharomyces pombe . Molec. Gen. Genet. 152, 319–324 (1977). https://doi.org/10.1007/BF00693086
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DOI: https://doi.org/10.1007/BF00693086