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  • 1990-1994  (24)
  • 1975-1979  (10)
  • 1
    Electronic Resource
    Electronic Resource
    Thermal ion-molecule reactions in oxygen-containing molecules. Condensation-elimination and addition reactions in simple aliphatic ketones (1978)
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 82 (1978), S. 639-643 
    Details
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/j100495a003
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  • 2
    Electronic Resource
    Electronic Resource
    Immobilization and Culture of Cells (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 613 (1990), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1990.tb18264.x
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  • 3
    Electronic Resource
    Electronic Resource
    Dose-Dependency of radiation on enzyme production inTrichoderma reesei (1993)
    Springer
    Radiation and environmental biophysics 32 (1993), S. 41-46 
    Details
    ISSN: 1432-2099
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract Effect of irradiation dose on the production of cellulase and amylase related enzymes inTrichoderma reesei was studied, in which post-irradiation time responce pattern was measured. The damage of the cells irradiated with certain irradiation doses (1.40±0.20x105, 2.20±0.10x105, 3.00±0.50 x 10 and 3.50±0.20 x 10 rad) was rapidly recovered. The increased enzyme production in the culture of the irradiated cells resulted from the recovery of radiation damage after irradiation. The function of cell growth was not affected by irradiation below dose of 5 x 105 rad, though the function of enzyme synthesis was drastically affected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01213129
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  • 4
    Electronic Resource
    Electronic Resource
    Immobilization of Streptomyces phaeochromogenes cells at a high concentration by radiation-induced polymerization of glass-forming monomers (1978)
    Springer
    Applied microbiology and biotechnology 6 (1978), S. 13-22 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Characteristics of Streptomyces phaeochromogenes cells immobilized by radiation-induced polymerization of 2-hydroxyethyl methacrylate at low temperatures were studied. It was found that very high concentrations of cells could be trapped effectively on the surface of the polymer matrix. Glucose isomerase activity of immobilized cells increased with increasing cell concentration. No cell leakage from the matrix was observed with repeated use, even at very high cell concentration and low monomer concentrations. The Km value of immobilized cells decreased with increasing cell concentration and with decreasing monomer concentration; it was close to that of intact cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00500852
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  • 5
    Electronic Resource
    Electronic Resource
    Adsorption of γ-globulin on polymer surfaces having various chemical and physical structures (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 41 (1990), S. 177-184 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The adsorption of human γ-globulin on polymer surfaces having various chemical and physical structures was studied. The γ-globulin adsorption on the crosslinked, rigid polymers of polyethyleneglycol dimethacrylate was considerably affected by the length of oxyethylene chains, in which the hydrophilicity of the polymers increased as the oxyethylene chains lengthened. The γ-globulin adsorption on the rigid polymers of tetraethyleneglycol dimethacrylate (or diacrylate) was lower than other soft polymers such as hydrogel, owing to the presence of oxyethylene chains. The polymer having a porous structure appeared to be a high adsorption material for the γ-globulin and the degree of the adsorption varied with pore size.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1990.070410115
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  • 6
    Electronic Resource
    Electronic Resource
    Enzyme immobilization by radiation-induced polymerization of 2-hydroxyethyl methacrylate at low temperatures (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 21 (1979), S. 847-861 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Enzyme immobilization by radiation-induced polymerization of hydrophilic glass forming monomers, such as 2-hydroxyethyl methacrylate, was studied. Enzyme radiation damage could be sufficiently retarded at low temperatures. The immobilized enzyme activity yield was markedly higher at low temperature than at higher temperature polymerization. At low temperatures the polymerized composite had a porous structure owing to ice crystallization which depends on the monomer concentration. It was deduced that the enzyme was partially trapped on the polymer surface, partially isolated in the pore, and partially occluded inside the polymer matrix. A decrease in activity caused by enzyme leakage was observed with repeated use in enzyme reactions where the composites had a large porosity. The activity yield showed a maximum at certain optimum porosities, i.e., at optimum monomer concentrations. Continuous enzyme reaction was preferably carried out using immobilized enzyme columns.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260210508
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  • 7
    Electronic Resource
    Electronic Resource
    Enzyme immobilization by radiation-induced polymerization of hydrophobic glass-forming monomers at low temperatures (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 21 (1979), S. 863-873 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Enzyme immobilization was studied by means of radiation-induced polymerization of hydrophobic glass-forming monomers at low temperatures. The polymerized hydrophobic composite was generally obtained in microspheric form. Enzymatic activity showed little decrease with repeated use in these systems. The particle size of the microsphere increased with increasing monomer concentration, and activity yield had a maximum at an optimum monomer concentration. Immobilization by copolymerization of hydrophilic and hydrophobic comonomers was also investigated and a maximum activity yield was found at a certain monomer concentration. A model scheme for immobilization at low temperatures was proposed and discussed.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260210509
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  • 8
    Electronic Resource
    Electronic Resource
    Radiation-induced decomposition and enzymatic hydrolysis of cellulose (1978)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 20 (1978), S. 1309-1315 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260200817
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  • 9
    Electronic Resource
    Electronic Resource
    Immobilization of Streptomyces phaerochromogenes by radiation-induced polymerization of glass-forming monomers (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 21 (1979), S. 679-688 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Immobilization of Streptomyces phaerochromogenes was radiation induced polymerization of 2-hydroxylate at low temperatures. Radiation damage of the enzyme could be avoided by choosing irradiation at low temperatures. The enzymatic activity of immobilized cells increased remarkably with a decrease in the irradiation temperature of about -24°C. In constrast to the case of cell-free enzyme immobilization, the most characteristic case was that in these immobilized cells, the enzymatic activity did not decrease with repeated use even in the composite obtained at much lower monomer concentrations. Another characteristic of immobilized cells was the increase in enzymatic activity in the initial stage of repeated use, which could be attributed to the swelling effect of the polymer matrix, thereby increasing the enzymatic activity to whole cells.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260210412
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  • 10
    Electronic Resource
    Electronic Resource
    Iodine-initiated, solid-state copolymerization of tetraoxane with 1,3-dioxolane in the presence of methylal. VI. Studies on copolymer composition (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 23 (1979), S. 3395-3409 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: In order to elucidate the mechanism of copolymerization of tetraoxane-1,3-dioxolane, the consumption rate of 1,3-dioxolane was measured by using gas chromatography, and the copolymer composition was determined by high-resolution NMR spectroscopy and differential scanning calorimetry. It was found that 1,3-dioxolane is rapidly consumed in the early stage of copolymerization if 1,3-dioxolane is added at once. The copolymer thus obtained is characterized by a heterogeneous distribution of 1,3-dioxolane in the copolymer chain. However, if 1,3-dioxolane is added continuously during the progress of the copolymerization, the distribution and content of 1,3-dioxolane in the copolymer chain was found to be very homogeneous.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1979.070231123
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