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  • Artikel  (65)
  • 1990-1994  (29)
  • 1980-1984  (36)
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  • 1
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 5 (1991), S. 0 
    ISSN: 1365-2958
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie , Medizin
    Notizen: We have isolated a hybrid gene, composed of the first 455 nucleotides of hisP and nucieotides 275–1107 of malK, the genes coding for the nucleotide-binding components of the high-affinity transport systems for histidine and maltose in Salmonella typhimurium, respectively. The fusion had occurred by recombination within 11 homologous base pairs located between the two DNA fragments. In the chimeric protein peptidic motifs A and B, proposed to be part of the nucleotide-binding fold, originate from HisP and MalK, respectively. Plasmid pES42-39, harbouring the hybrid gene, was shown to complement only a malK mutation but failed to complement a hisP deletion mutation. The chimeric protein was identified by immunoblotting as a protein with an apparent molecular mass of 49kDa. Removal of the C-terminal 77 amino acid residues from the chimeric protein resulted in the loss of function in transport. In contrast, 51 amino acid residues could be removed from the C-terminus of wild-type MalK without any effect. Upon overproduction the chimeric protein, as wild-type MalK, inhibited expression of the malB regulon. However, both truncated proteins, when overproduced, did not exhibit this activity. Based on these results, a tentative model of the functional domains of MalK is presented.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    ISSN: 1365-2958
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie , Medizin
    Notizen: In the framework of the European project aimed at the sequencing of the Bacillus subtilis genome the DNA region located between gerB (314°) and sacXV (333°) was assigned to the Institut Pasteur. In this paper we describe the cloning and sequencing of a segment of 97 kb of contiguous DNA. Ninety-two open reading frames were predicted to encode putative proteins among which only forty-two were found to display significant similarities to known proteins present in databanks, e.g. amino acid permeases, proteins involved in cell wall or antibiotic biosynthesis, various regulatory proteins, proteins of several dehydrogenase families and enzymes II of the phosphotransferase system involved in sugar transport. Additional experiments led to the identification of the products of new B. subtilis genes, e.g. galactokinase and an operon involved in thiamine biosynthesis.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    European journal of wildlife research 26 (1980), S. 126-132 
    ISSN: 1439-0574
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Beschreibung / Inhaltsverzeichnis: Summary In a wild boar enclosure in Austria, one individual several years old was observed smell marking and defending food. Apples were smell marked with urine and saliva. Young animals did not eat this food after having tested it by sniffing, although the marking ♂ did. The wild boar defended his feeding ground against men. In the process, he showed in a weaker or ritualised form behavioural patterns from the innate characteristics of parading, threatening and fighting. In addition, there is a report of an old boar in which acoustic impulses (the noises of a food container being used by another boar) triggered off a demonstration of rank and appropriation at the feeding ground. This trigger was more forceful than „avoidance of the enemy“. Mention is made of a case of imitation and learning in the overcoming of an obstacle.
    Kurzfassung: Résumé Dans un parc à sangliers situé en Autriche un sanglier mâle adulte a été observé lorsqu'il procédait à des marquages olfactifs et lorsqu'il défendait sa nourriture. Des pommes furent marquées olfactivement au moyen d'urine et de salive. Contrairement au mâle en question, des sujets juvéniles n'ont pas absorbé cette nourriture apreast en avoir goûté par voie olfactive. Le verrat défendit son lieu de nourrissage contre les humains. A cette occasion il manifesta, sous une forme atténuée ou ritualisée, un comportement spécifique de défi, de menace et de combat. On fait également état d'une démonstration de rang social et d'appropriation sur une aire de nourrissage par un sanglier mâle âgé déclenchée par des stimuli acoustiques simulant le bruit provoqué par l'utilisation de mangeoires par d'autres sangliers. Ce déclencheur était plus puissant que l'„évitement d'un ennemi“. On signale un cas d'imitation et d'apprentissage lors du franchissement d'un obstacle.
    Notizen: Zusammenfassung In einem österreichischen Wildschweingatter wurde ein mehrjähriger Keiler beim Duftmarkieren und Verteidigen von Futter beobachtet. Mit Harn und Speichel wurden Äpfel geruchlich markiert. Jungtiere haben dieses Futter nicht verzehrt, nachdem sie es olfaktorisch geprüft hatten, wohl aber das markierende ♂. Gegenüber Menschen verteidigte der Keiler seinen Futterplatz. Dabei zeigte er in abgeschwächter od. ritualisierter Form Verhaltensweisen aus dem innerartlichen Imponier-, Droh- und Kampfverhalten. Es wird ferner berichtet über einen alten Keiler, bei dem akustische Reize, die Geräusche eines von anderen Wildschweinen benutzten Futterbehälters, eine Rang- und Inbesitznahmedemonstration am Futterplatz auslösten. Dieser Auslöser war stärker als die „Feindvermeidung“. Ein Fall von Nachahmung und Lernen bei der Überwindung eines Hindernisses wird erwähnt.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    The European physical journal 39 (1980), S. 233-238 
    ISSN: 1434-6036
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract The characteristic ferroelectric dispersion of the complex dielectric constant of the ferroelectric AgNa(NO2)2 is measured as function of temperature and hydrostatic pressure. For the first time the temperature and pressure dependences of the static order parameter susceptibility and the order parameter relaxation time could be reproducibly determined. The results are interpreted in terms of a kinetic Ising model, whose parameters show a characteristic pressure dependence. The results are discussed on the basis of thermodynamic relations and related to the dynamical behaviour of other order-disorder ferroelectrics.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Journal of applied electrochemistry 22 (1992), S. 204-214 
    ISSN: 1572-8838
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Elektrotechnik, Elektronik, Nachrichtentechnik
    Notizen: Abstract One of the primary obstacles to be overcome for the development of economical fuel cells is the high cost of the membrane electrolyte. The currently favoured polymer electrolytes consist of poly(tetrafluoroethylene) backbone structures and poly(perfluorosulphonic acid) side chains. In an effort to find lower cost membranes, some radiation-grafted copolymer membranes were investigated. All the membranes contained poly(styrenesulphonic acid) side chains. Three different backbone polymer structures were studied: low-density poly(styrene), poly(tetrafluoroethylene)/poly(perfluoropropylene), and poly(tetrafluoroethylene). The results indicate that the membrane consisting of a poly(tetrafluoroethylene)/poly(styrenesulphonic acid) copolymer is a promising candidate as a fuel-cell electrolyte.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    Springer
    The European physical journal 296 (1980), S. 81-85 
    ISSN: 1434-601X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract The half-life of the 1/2− isomer in89Mo has been determined in an experiment where an enriched92Mo target was irradiated by 350 or 700 ms bursts of 60 MeV protons. Analysis of the gamma-ray spectra collected as a function of time between irradiations revealed gamma rays at 118.8 and 268.5 keV decaying with a 190±15 ms half-life. The hindrance ofE3 transitions inN=47 andZ=47 nuclides is discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Digitale Medien
    Digitale Medien
    Springer
    Human genetics 〈Berlin〉 65 (1984), S. 242-247 
    ISSN: 1432-1203
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The Dwl specificity was highly correlated with the serologically determined HLA-DR1 antigen in the Eighth International Histocompatibility Workshop 1980. By testing a large number of HLA-Dwl, DR1 defined homozygous typing cells (HTC) in a checkerboard primary mixed lymphocyte reaction, on a panel of about 30 HLA-DR1 heterozygous individuals, and in family segregation, three Dwl “subtypes” could be defined in association with certain HLA-A, -B, and -C-antigens. HTC TA, FRI, and FRA carrying the HLA haplotypes A11, B35, Cw4 or A3, B35, Cw4 in the homozygous state gave positive typing results with most HLA-DR1 positive panel members and stimulated only four other Dw1 HTCs (SRR≦35%). In contrast to this operationally “broad” specificity, Dw1-HTC-HEN (HLA-A2, B44, C-, homozygous) was non-stimulatory to all HTCs except one, but gave high responses against these, leading to the definition of a “narrow” specificity included in the “broad” one. Another such “narrow” specificity was represented by HTC FEE (HLA-A2, B27, Cw2 homozygous). Typing patterns with FEE were mostly different from those defined with other HTC. In family studies a specific typing pattern for this HTC could be shown to segregate with HLA. However, within some of these responses a contribution of the HLA haplotype in the trans position must be assumed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    ISSN: 1432-2048
    Schlagwort(e): Amyloplasts ; Enzyme conversion ; Phosphorylose (starch) ; Senescence ; Solanum ; Starch phosphorylase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Phosphorylase was purified from young and senescent potato tubers. Antibodies raised against the enzyme from young tubers crossreacted with phosphorylase from old tissue, although the latter exhibited different physico-chemical properties. In polyacrylamide gel electrophoresis it migrated with higher mobility, its subunit molecular weight was determined in the range of 40,000 in contrast to 100,000 of the phosphorylase in young tubers. The enzyme of senescent tubers displayed an isoelectric point of 5.4 different from the one of young tubers with 5.0, and the diffusion coefficients of the two enzymes varied. The appearance of the phosphorylase form typical for senescent tissue is connected with changes in the intracellular localization as revealed by immunofluorescence. Before massive starch accumulation is initiated, non-vacuolated subepidermal cells contain antigenically active material in their cytoplasm. During starch accumulation in fully differentiated storage parenchyma, only amyloplasts fluoresce, indicating the presence of adsorbed phosphorylase protein. Cytoplasmic phosphorylase can be detected in the continuance of senescence and, finally, after 16 months of tuber storage, the particle-bound enzyme had mostly disappeared. Simultaneously, we observed membrane destruction and decomposition on the ultrastructural level. The phosphorylase from senescent potatoes is a converted molecule and seems to be formed by proteolytic cleavage. The location of phosphorylase in the amyloplasts during starch synthesis indicates that it also plays a role in starch synthesis and not only in its degradation.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    Digitale Medien
    Digitale Medien
    Springer
    Planta 152 (1981), S. 177-180 
    ISSN: 1432-2048
    Schlagwort(e): Concanavalin receptors ; Endoplasmic reticulum ; Lepidium ; Statocytes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Using Concanavalin A (Con A) labeled with fluorescein isothiocyanate, we studied the intracellular localization of receptor molecules in the calyptra of 24-h dark-grown cress roots. Fixation in glutaraldehyde gave positive binding of the distal complex of the endoplasmic reticulum and the nucelus in the statocytes. In contrast, fixation in formaldehyde did not preserve the membrane-associated receptors, but revealed Con A affinity of the starch grain surface within the amyloplasts. Treatment of glutaraldehydefixed sections with non-ionic detergents led to partial solubilization of membrane components: the starch grain surface turned positive, though the positive binding of Con A to the endoplasmic reticulum and the nucleus remained unaffected. We therefore conclude that the Con A receptor in the membrane is a glycoprotein tightly inserted in other components of the compartment.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    Digitale Medien
    Digitale Medien
    Springer
    Probability theory and related fields 58 (1981), S. 125-138 
    ISSN: 1432-2064
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Mathematik
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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