Publication Date:
1980-03-28
Description:
Groups of three to four mice were gavaged with aqueous solutions of 2 milligrams of morpholine, after which they were exposed to nitrogen dioxide in inhalation chambers at concentrations of 0.2 to 50 parts per million for up to 4 hours. At sequential intervals during the exposure, mice were frozen and pulverized in liquid nitrogen, and the mice powder was extracted with ice-cold 35 percent aqueous methanol and dichloromethane; organic-phase concentrates were analyzed for N-nitrosomorpholine with a thermal energy analyzer interfaced to a gas chromatograph. The N-nitrosomorpholine yields, ranging up to about 2.3 micrograms per mouse, were time-dependent relative to the duration of exposure to nitrogen dioxide and dose-dependent relative to the concentrations of nitrogen dioxide; control levels (in mice that were gavaged with morpholine or distilled water and then exposed to air instead of nitrogen dioxide) were less than 5 nanograms per mouse. These preliminary studies demonstrate the in vivo nitrosating potential of nitrogen oxides.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Iqbal, Z M -- Dahl, K -- Epstein, S S -- New York, N.Y. -- Science. 1980 Mar 28;207(4438):1475-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7361099" target="_blank"〉PubMed〈/a〉
Keywords:
Amines/metabolism
;
Animals
;
Ascorbic Acid/pharmacology
;
Biotransformation
;
Dose-Response Relationship, Drug
;
Mice
;
Morpholines/*metabolism
;
Nitrogen Dioxide/antagonists & inhibitors/*metabolism
;
Nitrosamines/*metabolism
;
Time Factors
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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