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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 136 (1983), S. 42-48 
    ISSN: 1432-072X
    Keywords: Clostridium lortetii ; Endospores ; Gas vacuoles ; Butyric acid ; Fermentation ; Halophilic bacteria ; Dead Sea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A strain of Clostridium was isolated from Dead Sea sediment, differing from the previously described Clostridium types in its halophilic character. It required NaCl concentrations between 1 and 2 M, and optimal growth was found in 1.4–1.5 M NaCl at 30° C and in 1.7 M NaCl at 45° C. In sporulating cells gas vacuoles developed, generally near the developing terminal endospore only, and these vacuoles remained attached to the mature endospore after degeneration of the vegetative cell. Fermentation products included acetate, butyrate and hydrogen. Glucose and a few other carbohydrates stimulated growth, though they were poorly utilized. A new species name has been proposed for the organism: Clostridium lortetii.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 130 (1981), S. 185-187 
    ISSN: 1432-072X
    Keywords: Bacteriorhodopsin ; Halobacterium ; Dead Sea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A dense bloom of red halobacteria developed in the Dead Sea in the summer 1980, bacterial densities of up to 1.9 x107 cells ml-1 were observed. The population consisted of two types: pleomorphic, cup-shaped cells and rod-shaped cells. A high content of bacteriorhodopsin was found in the bloom (up to 0.4 nmol per mg protein). The rod-shaped Halobacterium was isolated and was shown to contain bacteriorhodopsin.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 136 (1983), S. 184-190 
    ISSN: 1432-072X
    Keywords: Halobacteria ; Magnesium tolerance ; Dead Sea bacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pleomorphic Halobacterium strains isolated from the Dead Sea (H. volcanii, H. marismortui) require high concentrations of divalent cations (75 mM Mg2+) for growth. When suspended in medium containing less than 50 mM Mg2+ cells lose their native shape within minutes and become spherical. This occurs even at elevated sodium chloride concentrations. Concomitant with the morphological changes, a high mlecular weight component which is positive in Coomassie Brilliant Blue and in periodate Schiff stain is released into the surrounding medium. At divalent cation concentrations lower than 100 mM magnesium cells were shown to lose their viability and their ability to incorporate amino acids. The potency of different divalent cations or their combinations to enable growth and stabilize morphology and viability was studied. It is suggested that different mechanisms underlie the divalent cation requirement of the different functions.
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  • 4
    ISSN: 1432-0983
    Keywords: Oversecretion mutants ; Protease defect ; Wall glucan defect ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two chromosomal mutations in yeast that result in oversecretion of the K1 killer toxin protein were examined. A recessive mutation in gene ski5 appears to lead to toxin oversecretion through a defect in a cell surface, PMSF-inhibited protease. A wild type killer strain degraded toxin following synthesis, and degradation could be partially prevented by addition of PMSF to the growth medium. The ski5 mutation caused an approximate ten fold oversecretion of toxin, similar to that seen in a PMSF-treated wild type culture, and no increased oversecretion in the presence of PMSF. The ski5 mutation caused oversecretion of other low molecular weight secreted proteins and appeared to oversecrete the α-factor pheromone, as judged by activity tests. The ski5 mutation was complemented by mutations in ski genes 1–4, and the mutant was not supersensitive to mating pheromones or K2 killer toxin. We also examined killer strains with a mutation in the nuclear gene krel which results in a defective (1→6)-β-D-glucan cell wall receptor for killer toxin. Such strains oversecrete toxin into the growth medium, but also, unexpectedly, oversecrete most other secreted proteins. The defect in (1→6)-β-D-glucan in these mutants appears to perturb the partitioning of secreted proteins between the cell wall and the medium.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Journal of applied electrochemistry 13 (1983), S. 473-487 
    ISSN: 1572-8838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Notes: Abstract It was shown previously that adsorption of ions at the electrical double layer of a high specific surface area carbon electrode can serve for water desalination. This is effected by assembling two such electrodes in a cell, cycling electronic charge between them and applying periodic synchronous pumping of the liquid through the cell. This process has been termed Electrochemical Parametric Pumping (ECPP). In the present article, modified charge coordinates, desalting efficiencies, isopotentiograms (analogous to adsorption isotherms) and optimization considerations of the two adsorptive electrode batch unit of the ECPP were derived and analysed on the basis of the propterties of each single electrode separately.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Journal of applied electrochemistry 13 (1983), S. 489-505 
    ISSN: 1572-8838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Notes: Abstract Multistage electrochemical parametric pumping has been successfully carried out for water desalination. Separation was effected by electroadsorption-desorption cycles of the ions into and from the electrical double layer of high surface carbon electrodes. At steady state and total reflux, the concentration ratio between the upper and lower heads of the electrochemical column was as high as 150. Two models for the build up of the concentration profile within the column are presented. The first is based on a solution of the two-phase mass transport equation using the proper boundary and initial conditions. The second treatment is based on the mixed cells assumptions. In both treatments, interphase equilibrium is assumed using the isopotentiograms as the specific equilibrium curves. Use is also made of a fast computer for the simulation of the electrochemical paramatric pumping cycles. The two models are in good agreement with the experimental results particularly in the cases where the initial concentration is high and interphase equilibrium is maintained.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Journal of optimization theory and applications 33 (1981), S. 25-36 
    ISSN: 1573-2878
    Keywords: Conjugate directions ; quadratic forms ; normal distributions ; probability assessment ; decision analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract A procedure is developed that enables the encoding of a subjectiven-dimensional joint normal probability density function through the assessment of its marginal means and variances andn(n−1)/2 conditional means. The new method is based on the theory of conjugate directions for quadratic forms, and it exploits the fact that normal distributions have quadratic equal-likelihood surfaces. Unlike previous approaches, this new method enables easy detection and resolution of inconsistencies in the assessments that could lead to an indefinite estimate of the covariance matrix.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Journal of optimization theory and applications 37 (1982), S. 137-147 
    ISSN: 1573-2878
    Keywords: Unconstrained optimization ; quasi-Newton methods ; self-scaling variable metric methods ; nonquadratic models
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract Recent attempts to assess the performance of SSVM algorithms for unconstrained minimization problems differ in their evaluations from earlier assessments. Nevertheless, the new experiments confirm earlier observations that, on certain types of problems, the SSVM algorithms are far superior to other variable metric methods. This paper presents a critical review of these recent assessments and discusses some current interpretations advanced to explain the behavior of SSVM methods. The paper examines the new empirical results, in light of the original self-scaling theory, and introduces a new interpretation of these methods based on anL-function model of the objective function. This interpretation sheds new light on the performance characteristics of the SSVM methods, which contributes to the understanding of their behavior and helps in characterizing classes of problems which can benefit from the self-scaling approach.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Journal of optimization theory and applications 43 (1984), S. 167-204 
    ISSN: 1573-2878
    Keywords: Nonlinear optimization ; quasi-Newton methods ; conjugate-directions algorithms ; indefinite quadratic forms ; saddlepoint problems
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract A new class of quasi-Newton methods is introduced that can locate a unique stationary point of ann-dimensional quadratic function in at mostn steps. When applied to positive-definite or negative-definite quadratic functions, the new class is identical to Huang's symmetric family of quasi-Newton methods (Ref. 1). Unlike the latter, however, the new family can handle indefinite quadratic forms and therefore is capable of solving saddlepoint problems that arise, for instance, in constrained optimization. The novel feature of the new class is a planar iteration that is activated whenever the algorithm encounters a near-singular direction of search, along which the objective function approaches zero curvature. In such iterations, the next point is selected as the stationary point of the objective function over a plane containing the problematic search direction, and the inverse Hessian approximation is updated with respect to that plane via a new four-parameter family of rank-three updates. It is shown that the new class possesses properties which are similar to or which generalize the properties of Huang's family. Furthermore, the new method is equivalent to Fletcher's (Ref. 2) modified version of Luenberger's (Ref. 3) hyperbolic pairs method, with respect to the metric defined by the initial inverse Hessian approximation. Several issues related to implementing the proposed method in nonquadratic cases are discussed.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Current microbiology 8 (1983), S. 225-230 
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Halobacterium sodomense, a halophilic bacterium from the Dead Sea, degraded starch to glucose by means of an extracellular amyloglucosidase with a temperature optimum of around 65°C in the presence of 1.4 M NaCl, and around 75°C in the presence of 3.9 M NaCl. The enzyme required salt concentrations higher than 1 M for optimal activity, NaCl, KCl, and MgCl2 being equally suitable as activators. The optimum pH was 7.5.H. sodomense culture supernatants showed only a very low maltose degrading activity. H. sodomense excreted amyloglucosidase constitutively, and relatively high activities were found in cultures grown in the absence of starch; when glucose was added to the growth medium, the amount of enzyme excreted into the medium decreased.
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