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  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Parasitology research 33 (1970), S. 355-357 
    ISSN: 1432-1955
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Parasitology research 35 (1971), S. 173-187 
    ISSN: 1432-1955
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The intestine of the infective larva consisted of a solid column of seven cells with no lumen. The cells, which seemed to be enzymatically inactive contained large deposits of glycogen and lipid indicating that the intestine probably functioned as a storage organ at this stage. The development of an intestinal lumen was apparent on the third day of infection when the larva was situated in the host liver. Acid and alkaline phosphatase and leucine amino peptidase activity was detected in the intestine at this stage but feeding had not commenced. Further development of the intestine occurred in the lung stage larva, 8 days after infection, the organ now being fully functional.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Parasitology research 36 (1971), S. 179-192 
    ISSN: 1432-1955
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Electron microscope observations of the “excretory” cell of the infective larva reveal that it contains a large nucleus surrounded by cytoplasm containing numerous organelles, multi-granular bodies, vesicles and granules typical of glandular cells. The proximal region of the “excretory” duct bears a number of scattered microvilli, on its adluminal surface, and the distal region is lined with a thin multilayered cuticle. In the liver stage larva 2 days after infection, 2 lateral “excretory” columns are present. These arise from the “excretory” cell body and extend posteriorly for about half the length of the intestine. Each column contains a narrow longitudinal canal surrounded by cytoplasm rich in mitochondria, granular endoplasmic reticulum, free ribosomes and large vacuoles. Evidence was obtained of the passage of substances through the wall of the canal but their chemical nature was not determined. Further extension of the lateral columns is seen in the 8-day, lung-stage larva, the columns now extending for more than two-thirds the length of the intestine. Their diameter is also increased but their internal structure is essentially similar to that of the 2-day liver-stage larva. The “excretory” duct which arises immediately anterior to the nucleus has a structure similar to that of the lateral columns for the first half of its length, the microvilli described in the infective larva being absent at this stage. The distal half of the duct is lined with cuticle.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Parasitology research 37 (1971), S. 255-266 
    ISSN: 1432-1955
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Electronmicroscope observations of the pharynx of the 2nd stage infective larva reveal that it is devoid of musculature and is therefore incapable of any pumping activity. By the 8th day when the larva is situated in the host lung however, the radial muscles are well developed and the organ appears to be fully functional. Active secretory glands are present in the pharynx of both the infective and lung stage larvae but those of the latter contain a greater variety of secretory bodies. It is suggested that the pharyngeal glands of the infective larva secrete lytic substances to facilitate host tissue penetration whilst the secretory products of the lung stage larva are concerned with a more diverse range of functions including feeding. No valve like structure was discernable at the pharyngeo-intestinal junction of the larvae recovered from the host lungs 8 days after infection. It is suggested that the cells of the pharyngeo-intestinal junction, function as a shock absorber between the highly mobile pharynx and the relatively static intestine.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    ISSN: 1432-1955
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary A study was made, over a period of 12 weeks, of the distribution of a population of immune-adapted worms that had established themselves in the small intestine of rats as a result of exposure to a number of small daily challenge infections with the nematode Nippostrongylus brasiliensis. Immature adapted worms were found to inhabit a greater length of the small intestine than immature primary infection worms. Mature worms, however, were restricted to the anterior two-fifths of the small intestine, the great majority of these infesting the region of the duodenum. The worms were not expelled from the intestine by an acute host-reaction, a large proportion of the worms still being present on the eighth week after the last larval challenge. Evidence was obtained that these immune-adapted worms favoured the duodenal environment rather than the other regions of the intestine and that this attraction for the duodenum was not affected by the immune state of the host.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    ISSN: 1432-1955
    Schlagwort(e): In vitro culture ; Anthelmintics ; Primary screening test ; Nippostrongylus brasiliensis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract A new in vitro test suitable for the large scale screening of chemical compounds for anthelmintic activity is described. The test which utilizes the fourth larval and adult stages ofNippostrongylus brasiliensis in a medium capable of supporting the growth and development of the parasite, detects selectively those compounds which possess either broad spectrum anthelmintic or specific anti-trichostrongyle activity. The screen is easy to operate requiring only minute quantities of experimental compound. It renders fully reproducible results which furthermore can be interpreted objectively. This is the first reported in vitro test directed against the parasitic stages of a nematode that is capable of detecting reliably the activity of a wide range of anthelmintics including thiophanate and all the benzimidazoles.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Digitale Medien
    Digitale Medien
    Springer
    Parasitology research 70 (1984), S. 655-661 
    ISSN: 1432-1955
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract In vitro screens employing newly excysted, 6- and 12-week-old flukes, in a medium permitting the linear growth of the parasites, were assessed. When exposed to certain known fasciolicides, newly excysted flukes were susceptible only to diamphenethide, the free amine of diamphenethide, emetine hydrochloride and albendazole. Older flukes were affected by a much wider range of compounds including the chlorinated hydrocarbons, the substituted phenols and the salicylanilides. However their susceptibility to diamphenethide and its active metabolite was decreased significantly. The activity of fasciolicides in these in vitro assays therefore closely parallels their activity in vivo. When several broad spectrum anti-nematode agents were evaluated against newly excysted flukes in these screens the benzimidazole, isothiocyanate, pyrimidine and imidazothiazole anthelmintics showed activity but 12 potent antiprotozoal agents were all inactive. It is concluded that these in vitro assays were useful for detecting any intrinsic activity that a compound might possess against flukes. Such activity could often be missed in conventional in vivo screens because of problems associated with host pharmacokinetics. Negative results from such in vivo screens could preclude the development of more bioavailable derivatives or pro-drugs as novel and useful fasciolicidal agents.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    Springer
    Parasitology research 70 (1984), S. 395-402 
    ISSN: 1432-1955
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract Approximately 60% of fourth-stage larvae ofNematospiroides dubius recovered from mice 6 days after infection, developed to the young adult stage when cultured over a 7-day period in a complex medium in vitro. Larvae at the late fourth stage of development were found to be highly susceptible to most broad spectrum anthelmintics under in vitro conditions, the benzimidazole, imidazothiazole, pyrimidine, isothiocyanate and macrocyclic lactone compounds all being active at very low concentrations. Narrow spectrum anthelmintics active only against ascarids, pinworms, filariae, cestodes or trematodes had little or no effect on these larvae. Ineffective also were those chlorinated hydrocarbon, substituted phenol and salicylanilide compounds known to affectHaemonchus but not trichostrongylid worms in general. It is concluded that in vitro assays employing larvae ofN. dubius are useful for the stringent screening of compounds for broad spectrum antitrichostrongyle activity. Used in conjunction with in vivo screens employingN. dubius in mice, they also afford means for detecting intrinsic activity against the parasite in a system free from any complicating host pharmacokinetics.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    Publikationsdatum: 2015-07-30
    Print ISSN: 0014-4851
    Digitale ISSN: 1741-2765
    Thema: Maschinenbau
    Publiziert von Springer
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
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