ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary Ethylenediamine (EDA) is toxic to the cyanobacterium Anabaena variabilis and inhibits nitrogenase activity. The inhibition of nitrogenase was prevented by pretreatment of cells with l-methionine-d,l-sulphoximine (MSX). Mutant strains of Anabaena variabilis (ED81, ED92), resistant to EDA, had low levels of glutamine synthetase (GS) biosynthetic activity compared with the wild type strain. ED92 had a low level of GS protein whereas ED81 had a similar level to that of the parent strain as estimated using antibodies against GS. Both strains fixed N2 and liberated NH4 + into the media. Following immobilization of the mutant strains, sustained photoproduction of NH4 + was obtained in air-lift reactors at rates of up to 50 μmol NH4 + mg chl a−1 h−1, which were comparable to the rates obtained when immobilized cyanobacteria were treated with MSX.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00266283
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