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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 89 (1985), S. 3185-3188 
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 93 (1989), S. 2891-2893 
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 91 (1987), S. 4024-4029 
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-203X
    Keywords: Gossypium hirsutum ; Somatic embryos ; Regeneration ; Morphogenesis ; Genotype
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Seventeen cultivars of cotton (Gossypium hirsutum L.) were evaluated for callus initiation and maintenance using 3 initiation media and 3 maintenance media. After a series of transfers of a 3% glucose media, calli were placed on a 3% sucrose medium. After several weeks calli were observed for the presence of embryo-like structures. Cultivars Coker 201 and Coker 315 were identified as embryogenic. Embryogenic callus has since been routinely obtained within 6 weeks by initiating callus on glucose media for 3–4 weeks followed by transfer to sucrose media. Histological examination has shown that embryos are derived from isodiametric, densely cytoplasmic cells and follow predictable patterns of development. Upon maturity, transfer to auxin-free media with reduced sucrose levels results in embryo germination. Regenerated plants can be transferred to greenhouse within 90 days of callus initiation.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-203X
    Keywords: Cotton ; Gossypium hirsutum L. ; Somatic embryogenesis ; storage proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The storage protein content of somatic embryos of Gossypium hirsutum L. cv. Coker 201 was determined using extinction level, antigen/antibody association detection methods. Mature storage protein was first detected in early globular-stage somatic embryos at a total concentration of 0.36% of the embryo protein mass. Tulip-stage and mature somatic embryos were comprised of 3.0% and 1.3% mature storage protein, respectively. Maximum storage protein synthesis was found to occur during early globular- and early heart-stages. During this period of development, significant levels of protein precursors were found also to accumulate. The pattern of storage protein synthesis, processing and accumulation paralleled the pattern that has been reported for the zygotic system, although somatic embryos accumulate storage protein at much earlier stages and to a lesser degree. The possibility of using complex biochemical pathways to monitor embryogenic systems in vitro is discussed.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 69 (1985), S. 279-284 
    ISSN: 1432-2242
    Keywords: Nuclear-cytoplasmic interaction ; Chlorophyll ; Glycine max L. ; Maternal inheritance ; Plastid ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genetic analysis of a yellow foliar mutant in soybean (Glycine max L. Merr.) showed maternal inheritance of the mutant phenotype designatedcyt-Y 3. The mutant was grown beside normal green sibs (cyt-G 3) under three different photosynthetic photon flux densities (PPFD), and samples were collected to determine pigment content and for electron microscopy analyses of plastid ultrastructure. The plastid ultrastructure ofcyt-Y 3 appeared normal at low PPFD and the carotenoid level ofcyt-Y 3 was also normal, but the chlorophyll content was only approximately one-third that ofcyt-G 3. Under medium and high PPFD,cyt-Y 3 plastids lacked a structured thylakoid, and total chlorophyll content was only 28% and 1% of normal, respectively; the carotenoid levels ofcyt-Y 3 also dropped to 33% and 2% of normal, respectively. These data indicate that the effect of high PPFD oncyt-Y 3 might result from a deficiency in a plastid membrane protein. The resulting changes in membrane configuration could then interfere with the accumulation or stabilization of chlorophylls and carotenoids, thereby resulting in the subsequent photooxidation of both at medium and high PPFD. This mutant could be useful in the study of thylakoid biosynthesis and pigment stabilization, or could provide a source of conditionally identifiable plastids for organelle segregation studies.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 77 (1989), S. 768-776 
    ISSN: 1432-2242
    Keywords: Chloroplast DNA ; RFLP ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restriction fragment length polymorphisms (RFLPs) have been used to detect intragenic sequence diversity in Glycine subgenus soja chloroplast DNA. The distribution of these RFLPs allow Glycine max and G. soja accessions to be grouped according to cytoplasmic genetic relatedness. DNA clones from mung bean chloroplast DNA were used to locate the RFLPs to specific regions of the chloroplast genome. In the course of the experiments, several previously unobserved RFLPs were also identified. At least six molecular changes were detected, including both restriction site loss or gain and insertion/deletion events. Three of the fragment polymorphisms detected are due to changes in the juncture region between one inverted repeat region and the large single-copy region. Probes detecting polymorphisms in three representative soybean genotypes were used to screen additional cultivars and Plant Introductions. The distribution of RFLP patterns in these accessions were consistent with the patterns of previously described cytoplasmic groupings, with the exception of one accession, which formed a new plastome group.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 77 (1989), S. 786-792 
    ISSN: 1432-2242
    Keywords: Restriction fragment length polymorphism Glycine max ; Glycine soja
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Fifty-eight soybean accessions from the genus Glycine, subgenus Soja, were surveyed with 17 restriction fragment length polymorphism (RFLP) genetic markers to assess the level of molecular diversity and to evaluate the usefulness of previously identified RFLP markers. In general, only low levels of molecular diversity were observed: 2 of the 17 markers exhibited three alleles per locus, whereas all others had only two alleles. Thirty-five percent of the markers had rare alleles present in only 1 or 2 of the 58 accessions. Molecular diversity was least among cultivated soybeans and greatest between accessions of different soybean species such as Glycine max (L.) Merr. and G. soja Sieb. and Zucc. Principal component analysis was useful in reducing the multidimensional genotype data set and identifying genetic relationships.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    International journal of thermophysics 9 (1988), S. 265-271 
    ISSN: 1572-9567
    Keywords: automation ; computer control ; dilatometer ; thermal expansion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The method used to automate two dual push-rod dilatometers is presented. The two dilatometers are operated simultaneously under computer control and the data are collected concurrently. The computer system, a PDP 11, is part of a laboratory network where any one of four systems can control the dilatometers. The thermal expansion results are calculated and displayed in real time.
    Type of Medium: Electronic Resource
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  • 10
    Publication Date: 1989-04-01
    Print ISSN: 0022-3654
    Electronic ISSN: 1541-5740
    Topics: Chemistry and Pharmacology , Physics
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