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  • 1
    Electronic Resource
    Electronic Resource
    Hairy root transformation in alfalfa (Medicago sativa L.) (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 523-530 
    Details
    ISSN: 1432-2242
    Keywords: Hairy root ; Medicago sativa L. ; Phenotypic variation ; Agrobacterium rhizogenes ; Genetic transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The widely cultivated forage legume alfalfa (Medicago sativa L.) was transformed with the agropine type Agrobacterium rhizogenes NCPPB 1855. Sterile root and callus cultures were derived from tumorous hairy roots which were easily obtained independent of the plant variety or genotype. Plant regeneration, via somatic embryogenesis, was achieved only when a selected alfalfa line, characterized by high regenerative capability, was utilized. Genetic transformation was confirmed by the presence of agropine and T-DNA. Phenotypic alterations, mainly affecting the root system, were observed in transformed plants. The possibility that T-DNA-induced variations could be useful in the improvement of M. sativa is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00289189
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  • 2
    Electronic Resource
    Electronic Resource
    Time-related behaviour of phenotypic variation in Lotus corniculatus regenerants under field conditions (1985)
    Springer
    Euphytica 34 (1985), S. 619-623 
    Details
    ISSN: 1573-5060
    Keywords: Lotus corniculatus ; birdsfoot trefoil ; tissue culture ; somaclonal variation ; alteration stabiliy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Plants of Lotus corniculatus regenerated from single genotype-derived calli showed for several agronomic and morphological traits a variation similar to that present in the original population. Comparisons of the results obtained under field conditions during the 2nd and 3rd year from plant regeneration show: a) the maintenance of significant variance within callus-derived population for several traits examined; b) the total disappearance of ‘minus variants’ indicating a time-related decrease of depressive in vitro effects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00035397
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  • 3
    Electronic Resource
    Electronic Resource
    Agrobacterium rhizogenes T-DNA genes capable of inducing hairy root phenotype (1987)
    Springer
    Molecular genetics and genomics 209 (1987), S. 475-480 
    Details
    ISSN: 1617-4623
    Keywords: Agrobacterium ; Hairy root ; T-DNA ; Transgenic tobacco
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Segments of the TL-DNA of the agropine type Ri plasmid pRi 1855 encompassing single and groups of open-reading frames were cloned in the Ti plasmid-derived binary vector system Bin 19. Leaf disc infections on Nicotiana tabacum led to transformed plants, some of which showed typical hairy root phenotypes, such as the wrinkled leaf morphology, excessive and partially non geotropic root systems and the ability of leaf explants to differentiate roots in a hormone-free culture medium. Particularly interestingly, most of these traits were shown by plants transformed with a TL-DNA segment encompassing the single ORF 11, corresponding to the rolB locus. Hairy root can be induced by this latter T-DNA segment on wounded stems of tobacco plants; hairy root induction on carrot discs requires, on the contrary, a more complex complement of TL-DNA genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331152
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