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  • 1985-1989  (5)
Collection
Publisher
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Immunofluorescent localization ofSchistosoma mansoni circulating cathodic antigen in tissues of infected mice using monoclonal antibody (1985)
    Springer
    Parasitology research 71 (1985), S. 317-323 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract In the present study the kinetics of the uptake and deposition of the major circulating cathodic antigen (CCA) ofSchistosoma mansoni in liver, spleen, and kidney ofS. mansoni infected Swiss mice was investigated in relation to the duration of infection and infection dose (50, 100, 200 cercariae). The presence of antigen was studied with a direct immunofluorescence reaction on frozen sections of the mouse organs, using a fluorescein isothiocyanate (FITC)-labelled mouse IgM monoclonal antibody recognizing a repeating epitope of CCA. CCA was demonstrable from 2 weeks post infection (p.i.) onwards in Kupffer cells in the liver, from 3–4 weeks p.i. onwards in macrophages in the marginal zones in the spleen and from 8 weeks p.i. onwards in kidney glomeruli. The immunofluorescence reactions on CCA in kidney glomeruli, however, remained relatively weak.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00928334
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  • 2
    Electronic Resource
    Electronic Resource
    Schistosoma mansoni: impaired clearance of model immune complexes consisting of circulating anodic antigen and monoclonal IgG1 in infected mice (1988)
    Springer
    Parasitology research 74 (1988), S. 356-362 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The clearance of schistosome-specific model immune complexes (IC) consisting of circulating anodic antigen (CAA), a gut-associated excretory-secretory antigen, and radiolabeled monoclonal antibody (IgG1) was investigated in mice with a light and heavy Schistosoma mansoni infection and in noninfected control animals. The size analysis of the in vitro prepared and injected IC, as determined by density gradient centrifugation, revealed a wide peak at 11S. In infected animals the injected IC were cleared at a significantly lower rate than in control mice. This was attributed to a decreased uptake of IC by the liver in infected mice. In heavily infected mice, 30 min after injection of 11S IC, 8S, 11S, and 〉11S IC were present in the serum, whereas only small 8S IC were detected in the serum of lightly infected animals and noninfected controls. Immune complexes were also present in the serum of heavily infected mice 30 min after injection of antibody and were detectable as 11S and 〉11S IC. The importance of this study is twofold. First, these results show that schistosome-specific monoclonal antibodies can be used in the production of model immune complexes applicable in clearance studies. Second, our findings might be of importance when the possible pathogenicity of circulating IC in schistosomiasis is considered.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00539458
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  • 3
    Electronic Resource
    Electronic Resource
    Schistosomiasis and in vitro transdifferentiation of murine peritoneal macrophages into fibroblastic cells (1989)
    Springer
    Parasitology research 76 (1989), S. 150-161 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We developed a method for avoiding contamination by fibroblasts when cultures of peritoneal cells are initiated. Macrophages were identified by immunogold detection [light microscope, transmission (TEM) and scanning (SEM) electron microscopes] of membrane antigens (Mac-1+, Thy-1,2−), non-specific esterase activity and ultrastructural features (TEM). As compared with controls, the yield of peritoneal macrophages was 2- and 12-fold higher, respectively, in acutely and chronically infected mice. In all, 30 “chronic”, 18 “acute” and 18 control cultures were followed up. At a given cell-density seeding, the decline of control, “acute” and “chronic” cultures starts at about day 10, 15, and 27, respectively. In “chronic” cultures only, fibroblast-like cells appear from day 6 onwards; their number increases with time. Cells showing characters intermediary between macrophages and fibroblasts were observed. We suggest that fibroblast-like cells result from the in vitro transdifferentiation of a limited number of in vivo committed macrophages.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00930838
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  • 4
    Electronic Resource
    Electronic Resource
    Immunohistochemical characterization of the mononuclear cells in the brain of the rat with an experimental chronicTrypanosoma brucei gambiense infection (1989)
    Springer
    Parasitology research 75 (1989), S. 251-256 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The distribution of T-cell subsets, B cells, and class II MHC antigens was examined within the CNS of rats chronically infected withTrypanosoma brucei gambiense, using appropriate mouse monoclonal antibodies. The mononuclear infiltrates of the leptomeninges and the perivascular areas (Virchow-Robin spaces) were composed of IgM-producing plasma cells and Mott cells and T-helper/inducer cells. Cells defined phenotypically as suppressor/cytotoxic T cells were rare. Anti-Ia reactive cells were also abundant in these inflammatory lesions and in the white matter, representing Ia-expressing neuroglial cells, B cells, activated T cells, and macrophages. The Ia-positive neuroglial cells, possibly acting as accessory cells, associated with numerous T-helper/inducer cells and cells from the B-cell lineage, suggest that a T-dependent B-cell immune response can be initiated within the CNS of rats with a chronicT. b. gambiense infection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00931808
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  • 5
    Electronic Resource
    Electronic Resource
    Autoantibodies to intermediate filaments in experimental infections withTrypanosoma brucei gambiense (1986)
    Springer
    Parasitology research 72 (1986), S. 443-452 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Sera from rats with chronicTrypanosoma brucei gambiense infection were tested for autoantibodies by an indirect immunofluorescence assay. All the sera contained IgM autoantibodies which reacted with blood vessel walls. On cultured vascular smooth muscle cells positive sera reacted with cytoplasmic filaments which were rearranged into perinuclear coils of filaments in colcemid-pretreated smooth muscle cells. These observations strongly suggest that the cytoplasmic autoantigens are intermediate filaments (I.F.). It is probable that the anti-intermediate filament autoantibodies result from polyclonal lymphocyte activation, since in rats experimentally infected withT.b. gambiense the appearance of these autoantibodies occurs already 1 week post-infection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00927888
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