ISSN:
1052-9306
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The feasibility of using specifically deuterated polyunsaturated fatty acyl pyrrolidides for the mass spectrometric localization of double bonds is analysed in detail. Two separate isotopic effects are considered, which affect the analysis: first, the chemical isotope effect that occurs during deuteration with 2H2-diimine; and second, 2H/H isotope effects that take place within the mass spectrometer. An unusual four-carbon-unit elimination reaction has been identified in long-chain pyrrolidides (〉C20), and this may give rise to confusing sequence fragment ion data. We conclude that this method may give correct results for up to six double bonds under certain circumstances, but that the variables that need to be considered include spectrometer geometry adn thus the type of instrument used.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bms.1200130809
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