Summary
Treatment of resident peritoneal macrophages of rats with small unilamellar vesicles of dipalmitoylphosphatidylcholine (DPPC SUV) potentiated their activation for tumor cell lysis by endotoxins. The fluorescence polarization of diphenylhexatriene (DPH) embedded in rough endoplasmic reticulum membranes isolated from DPPC SUV-treated macrophages was enhanced. The average fluorescence lifetime of DPH and the rotational correlation time deduced from anisotropy decay were unchanged, whereas the residual anisotropy and hence the order parameter were increased. The measurement of the fluorescence anisotropy of DPH as a function of the temperature showed a phase transition. No phase transition was observed in the rough endoplasmic reticulum membranes of macrophages either treated or not treated with cholesterol/DPPC SUV (1/1; mol/mol). The synergistic effect of DPPC SUV on the tumoricidal activity of macrophages induced by endotoxins appears to be correlated with the changes in the properties of the rough endoplasmic reticulum membranes. Both effects were transient; they had the same kinetics of induction and reversion, and they were both inhibited by cholesterol.
Similar content being viewed by others
References
Beutler, B., Krochin, N., Milsark, I.W., Luedke, C., Cerami, A. 1986. Control of cachectin (tumor necrosis factor) synthesis: Mechanisms of endotoxin resistance.Science 232:977–980
Brasitus, T.A., Tall, A.R., Schachter, D. 1980. Thermotropic transitions in rat intestinal plasma membranes studied by differential scanning calorimetry and fluorescence polarization.Biochemistry 19:1256–1261
Chapman, D., Urbina, J., Keough, K.M. 1974. Biomembrane phase transitions. Studies of lipid-water systems using differential scanning calorimetry.J. Biol. Chem. 249:2512–2521
Chen, L.A., Dale, R.E., Roth, S., Brand, L. 1977. Nanosecond time-dependent fluorescence depolarization of diphenylhexatriene in dimyristoyllecithin vesicles and the determination of “microviscosity”.J. Biol. Chem. 252:2163–2169
Dijkstra, J., Mellors, J.W., Ryan, J.L., Szoka, F. 1987. Modulation of the biological activity of bacterial endotoxin by incorporation into liposomes.J. Immunol. 138:2663–2670
Fidler, I.J., Barnes, Z., Fogler, W.E., Kirsh, R., Bugelski, P., Poste, G. 1982. Involvement of macrophages in the eradication of established metastases following intravenous injection of liposomes containing macrophage activators.Cancer Res. 42:496–501
Gallais, J., Vincent, M., Alfsen, A. 1982. Dynamic structure of bovine adrenal cortex microsomal membranes studied by time-resolved fluorescence anisotropy of all-trans-1,6-diphenyl-1,3,5-hexatriene.J. Biol. Chem. 257:4036–4041
Hammerstrom, J. 1982. Soluble cytostatic factor(s) released from human monocytes. I. Production and effect on normal and transformed human target cells.Scand. J. Immunol. 15:311–318
Hare, F., Lussan, C. 1978. Mean viscosities in microscopic systems and membrane bilayers.FEBS Lett. 94:321–325
Heyn, M.P. 1979. Determination of lipid order parameters and rotational correlation times from fluorescence depolarization experiment.FEBS Lett. 108:359–364
Hildenbrand, K., Nicolau, C. 1979. Nanosecond fluorescence anisotropy decays of 1,6-diphenyl-1,3,5-hexatriene in membranes.Biochim. Biophys. Acta 553:365–377
Huang, L., Ozato, K., Pagano, R.E. 1978. Interactions of phospholipid vesicles with murine lymphocytes. I. Vesicle-cell adsorption and fusion as alternate pathways of uptake.Membr. Biochem. 1:1–25
Jacobson, K., Papahadjopoulos, D. 1975. Phase transitions and phase separations in phospholipid membranes induced by changes in temperature, pH, and concentration of bivalent cations.Biochemistry 14:152–161
Jeannin, J.F., Olsson, N.O., Martin, F. 1983. Liposome induction or enhancement of macrophage-mediated cancer cell lysis.Int. J. Cancer 31:75–80
Jeannin, J.F., Reisser, D., Lagadec, P., Olsson, N.O., Martin, F. 1985. Synergistic effect of liposomes and endotoxins on the activation of rat macrophages tumoricidal activity.Immunobiology 170:211–231
Kawato, S., Kinosita, K., Jr., Ikegami, A. 1977. Dynamic structure of lipid bilayers studied by nanosecond fluorescence technique.Biochemistry 16:2319–2324
Kinosita, K., Jr., Kawato, S., Ikegami, A. 1977. A theory of fluorescence polarization decay in membranes.Biophys. J. 20:289–305
Kinosita, K., Jr., Kataoka, R., Kimura, Y., Gotoh, O., Ikegami, A. 1981. Dynamic structure of biological membranes as probed by 1,6-diphenyl-1,3,5-hexatriene: A nanosecond fluorescence depolarization study.Biochemistry 20:4270–4277
Klein, R., Ballini, J.P., Tatischeff, I. 1982. Photophysics of indole in tetramethylsilane solution. Is there a photoionization threshold?J. Phys. Chem. 86:4400–4404
Koizumi, K., Shimizu, S., Koizumi, K.T., Nishida, K., Sato, C., Ota, K., Yamanaka, N. 1981. Rapid isolation and lipid characterization of plasma membranes from normal and malignant lymphoid cells of mouse.Biochim. Biophys. Acta 649:393–403
Kramers, M.T.C., Patrick, J., Bottomley, J.M., Quinn, P.J., Chapman, D. 1980. Studies of liposomes interactions with rat thymocytes.Eur. J. Biochem. 110:579–585
Kull, F.C., Cuatrecasas, P. 1984. Necrosin: Purification and properties of a cytotoxin derived from a murine macrophage-like cell line.Proc. Natl. Acad. Sci. USA 81:7932–7936
Lakowicz, J.R., Prendergast, F.G., Hogen, D. 1979. Differential polarized phase fluorimetric investigations of diphenylhexatriene in lipid bilayers. Quantitation of hindered depolarizing rotations.Biochemistry 18:508–519
Lentz, B.R., Barenholz, Y., Thompson, T.E. 1976. Fluorescence depolarization studies of phase transitions and fluidity in phospholipid bilayers. 1. Single component phosphatidylcholine liposomes.Biochemistry 15:4521–4528
Lipari, G., Szabo, A. 1980. Effect of vibrational motion on fluorescence depolarization and nuclear magnetic resonance relaxation in macromolecules and membranes.Biophys. J. 30:489–506
Livingstone, C.J., Schachter, D. 1980. Lipid dynamics and lipidprotein interactions in rat hepatocytes plasma membranes.J. Biol. Chem. 255:10902–10908
MacKay, R.J., Russel, S.W. 1986. Protein changes associated with stages of activation of mouse macrophages for tumor cell killing.J. Immunol. 137:1392–1398
Margolis, L.B. 1984. Cell interaction with model membranes probing, modification and stimulation of cell surface functions.Biochim. Biophys. Acta 779:161–189
Martin, F., Caignard, A., Jeannin, J.F., Leclerc, A., Martin, M.S. 1983. Selection by trypsin of two sublines of rat colon cancer cells forming progressive or regressive tumors.Int. J. Cancer 32:623–627
Matthews, N. 1984. Anti-tumor cytotoxin from macrophages: No correlation between cytotoxin adsorption by tumour cell lines and their cytotoxin susceptibility.Immunology 53:537–543
Meltzer, M.S. 1981. Macrophage activation for tumor cytotoxicity: Characterization of priming and trigger signals during lymphokine activation.J. Immunol. 127:179–183
Morland, B., Morland, J. 1978. Selective induction of lysosomal enzyme activities in mouse peritoneal macrophages.J. Reticuloendothel. Soc. 23:469–477
Ozato, K., Huang, L., Pagano, R.E. 1978. Interactions of phospholipid vesicles with murine lymphocytes. II. Correlation between altered surface properties and enhanced proliferatives response.Membr. Biochem. 1:27–42
Papahadjopoulos, D., Jacobson, K., Nir, S., Isac, T. 1973. Phase transition in phospholipid vesicles. Fluorescence polarization and permeability measurements concerning the effect of temperature and cholesterol.Biochim. Biophys. Acta 311:330–348
Petty, H.R., McConnell, H.M. 1983. Cytochemical study of liposome and lipid vesicle phagocytosis.Biochim. Biophys. Acta 735:77–85
Poste, G. 1980. The interactions of lipid vesicles (liposomes) with cultured cells and their use as carriers for drugs and macromolecules.In: Liposomes in Biological Systems. G. Gregoriadis and A.C. Allison, editors. pp. 101–151. Wiley and Sons, New York
Poste, G., Papahadjopoulous, D. 1978. The influence of vesicle membrane properties on the interaction of lipid vesicles with cultured cells.Ann. N. Y. Acad. Sci. 308:164–181
Ryan, J.L., Yohe, W.B., Morrison, D.C. 1980. Stimulation of peritoneal cell arginase by bacterial lipopolysaccharides.Am. J. Pathol. 99:451–462
Sandra, A., Paltzer, W.B., Thomas, M.J. 1981. Morphological differentiation of murine neuroblastoma induced by liposomes.Exp. Cell Res. 132:473–477
Sene, C., Genest, D., Obrenovitch, A., Wahl, P., Monsigny, M. 1978. Pulse fluorimetry of 1,6-diphenyl-1,3,5-hexatriene incorporated in membrane of mouse leukemic L1210 cells.FEBS Lett. 88:181–186
Shinitzky, M., Barenholz, Y. 1974. Dynamics of the hydrocarbon layer in liposomes of lecithin and sphingomyelin containing dicetylphosphate.J. Biol. Chem. 249:2652–2657
Shinitzky, M., Barenholz, Y. 1978. Fluidity parameters of lipid regions determined by fluorescence polarization.Biochim. Biophys. Acta 515:367–394
Sone, S., Lopez-Berestein, G., Fidler, I.J. 1985. Kinetics and function of tumor cytotoxic factor(s) produced by human blood monocytes activated to the tumoricidal state.J. Natl. Cancer Inst. 74:583–590
Stevenson, H.C., Foon, K.A., Sugarbaker, P.H. 1986. Ex vivo activated monocytes and adoptive immunotherapy trials in colon cancer patients.Trans. Med.: Rec. Tech. Adv. 211:75–82
Suurkuusk, J., Lentz, B.R., Barenholz, Y., Bittonen, R.L., Thompson, T.E. 1976. A calorimetric and fluorescent probe study of the gel-liquid crystalline phase transition in small single-lamellar dipalmitoylphosphatidylcholine vesicles.Biochemistry 15:1393–1401
Tatischeff, I., Klein, R. 1976. Fluorescence quantum yield of the aromatic amino-acids as a function of excitation wavelength.In: Excited States of Biological Molecules. J.B. Birks, editor. pp. 375–387. J. Wiley and Sons, New York
Varesio, L. 1985. Imbalanced accumulation of ribosomal RNA in macrophages activated in vivo or in vitro to a cytolytic stage.J. Immunol. 134:1262–1262
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jeannin, J.F., Klein, R., Reisser, D. et al. Correlation between the synergistic effect of liposomes and endotoxins on the activation of macrophage tumoricidal activity and the effect of liposomes on the rough endoplasmic reticulum of macrophages. J. Membrain Biol. 104, 107–118 (1988). https://doi.org/10.1007/BF01870923
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01870923