ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    The use of submicroscopic gold particles combined with video contrast enhancement as a simple molecular probe for the living cell (1986)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 6 (1986), S. 105-113 
    Details
    ISSN: 0886-1544
    Keywords: video-microscopy ; colloidal gold ; immunocytochemistry ; microtubules ; receptors ; saltatory motion ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We describe a new approach to probe the molecular biology of the living cell that uses small colloidal gold particles coupled to specific ligands. They are visualized in cells by bright-field, video enhanced contrast microscopy. We describe the basic aspects of the technique and provide examples of applications to intracellular motility, cell membrane dynamics, receptor translocation, internalization, and intracellular routing. We also provide examples of the use of this approach in immunospecific labelling of cells and tissue sections.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970060207
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Effect of follicle-stimulating hormone on metabolism and maturation in Bufo arenarum oocytes (1989)
    New York, NY : Wiley-Blackwell
    Gamete Research 23 (1989), S. 349-356 
    Details
    ISSN: 0148-7280
    Keywords: FSH ; ovary ; amphibian ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: In the fully grown Bufo arenarum oocyte, carbohydrate breakdown during the autumn-winter season is accomplished mainly through the glycolytic pathway followed by the krebs cycle. During the breeding season (spring-summer), carbohydrates are used mainly through the pentose phosphate cycle and through the variant of the Krebs cycle known as the glutamic aspartic cycle.The metabolism operating in the oocytes was determined using the following paramenters: 1) the capacity of isolated mitochondria to oxidize citrate and fumarate; 2) the enzymatic activities of phosphofructokinase (PEK) and glucose-6-phosphate dehydrogenase (G-6-PDH); and 3) cirate and ATP compartmentalization.The present paper shows that follicle-stimulating hormone (FSH) would be one of the factors responsible for summer metabolism, since ovary fractios obtained from winter specimens treated with the hormone acquired the metabolic characteristics corresponding to oocytes obtained from breeding-season animals from dose-response, and response in the function of time curves, it could be assumed that the optimum doses and times were 0.1 μg FSH/ml of incubation medium and 30 min treatment, respecitively.The metabolic effect of FSH upon oocytes could be mediaated by the adenylate cyclase cAMP system, since treatment of ovric fractions with cAMP 10-3 M reproduced the effects obtained with the hormone. In addition, 0.02 mg/ml tetracyline proved to block the effect of FSH. A direct metabolic action of FSH on body cavity oocytes (without follicle cells) was observed when submitting these oocytes to the same hormonal treatment.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120230311
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Quantitative electromyography of the masticatory muscles of Pteropus giganteus (Megachiroptera) (1988)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 196 (1988), S. 73-106 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Mastication has been studied by cinematography and quantitative electromyography while flying foxes, Pteropus giganteus, were freely feeding on standardized pieces of apple, soaked raisin, and banana.The primarily orthal mandibular movements are caused by mainly bilaterally symmetrical firing of all the masticatory muscles. Asymmetric activity in the superficial and deep masseter and medial pterygoid causes slight protrusion early in opening. Slight lateral deviations at the end of opening and at the start of closing are caused by asymmetric and asynchronous activity in the pterygoids and digastrics, and by asynchronous firing of the deep temporalis and zygomaticomandibularis. Food consistency affects movement characteristics as well as characteristics of muscular activity.In this study electromyograms were digitized and the number of spikes and mean amplitude per interval (set by the filming rate) recorded. Although a significant correlation exists between these descriptors, the product thereof appears to be the best predictor of certain kinematic variables (cycle length and maximum excursion of the mandible). On the other hand, the changes in magnitude of muscular activity as a function of the position of a cycle in the reduction sequence and as a function of food consistency are more translated in a variation of the mean amplitude than in a variation of the number of spikes per interval. Observed variation differs among muscles studied. It is most apparent in the superficial and deep masseter and least in the temporalis and zygomaticomandibularis.Late cycles of apple and raisin mastication are long and exhibit large gapes but almost no anterior movement. The adductor activity frequently shows a synchronized, pulsatile pattern leading to an unfused tetanus.
    Additional Material: 16 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051960107
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Epidermal growth factor receptor expression during morphological differentiation of pheochromocytoma cells, induced by nerve growth factor or dibutyryl cyclic AMP (1987)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 131 (1987), S. 409-417 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Rat pheochromocytoma cells (clone PC12) possess functional surface receptors for both nerve growth factor (NGF) and epidermal growth factor (EGF). PC12 cells respond to NGF as well as to dibutyryl cyclic AMP (dbcAMP) by arrest of cell proliferation and initiation of morphological differentiation, while EGF acts as a mitogen. Exposure of PC12 cells to NGF for several days resulted in a complete loss of rapid EGF responses, such as membrane ruffling and activation of active K+ transport. EGF binding studies revealed that this loss of EGF responses was due to an almost complete reduction of the number of EGF binding sites. In contrast, exposure of PC12 cells to dbcAMP for 2 days did not affect the rapid EGF responses, despite the morphological differentiation. Moreover, EGF binding studies demonstrated a twofold increase in the number of high-affinity binding sites and a small increase in the number of low-affinity sites. In addition, exposure of the cells to dbcAMP caused a twofold increase of EGF-receptor phosphotyrosine kinase activity. These results indicate that neither EGF-binding or the presence of EGF receptors nor the rapid EGF responses are sufficient for persistent proliferation, on one hand, or sufficient to avoid morphological differentiation, on the other.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041310313
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Effects of bovine mammary alpha-lactalbumin on hyperactivation and sperm-zona pellucida binding of mouse spermatozoa (1989)
    New York, NY : Wiley-Blackwell
    Gamete Research 24 (1989), S. 415-426 
    Details
    ISSN: 0148-7280
    Keywords: galactosyltransferase ; capacitation ; epididymis ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The effects of bovine mammary alpha-lactalbumin on the motility and zona-binding characteristics of mouse sperm were investigated. Two properties of sperm associated with capacitation, hyperactivated motility, and the ability of sperm to bind to the zona pellucida of oocytes were shown to be suppressed by alpha-lactalbumin. These inhibitory effects were not accompanied by changes in the percentage of motile cells or by differences in the velocity parameters of the hyperactivated and non-hyperactivated spermatozoa. Bovine serum albumin prevented and reversed the alpha-lactalbumin-induced suppression of hyperactivation. Sperm-zona pellucida binding was partially restored by lowering the alpha-lactalbumin concentration in the medium in which sperm were allowed to bind to the zona pellucida. The results suggest that mouse sperm are decapacitated by bovine mammary alpha-lactalbumin. The counteracting effect of bovine serum albumin to the suppressive action of alpha-lactalbumin on the flagellum suggests the involvement of a mechanism different from the action of alpha-lactalbumin on the sperm head inhibiting binding to the zona pellucida.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120240408
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Orthogonal-field-alternation gel electrophoresis banding patterns of DNA from yeasts (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 193-204 
    Details
    ISSN: 0749-503X
    Keywords: Orthgonal-field-alternation gel electrophoresis ; karyotyping ; ascomycetous yeasts ; basidiomycetous yeasts ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Chromosomal DNAs from various yeast species were separated by orthogonal-field-alternation gel electrophoresis (OFAGE). To this end we developed a spheroplasting and lysis method to obtain intact DNA from both ascomycetous and basidiomycetous yeasts. The OFAGE banding patterns of 22 ascomycetous and four basidiomycetous yeast strains were compared. The strains represented species from the genera: Brettanomyces, Candida, Croococcus, Filobasidiella, Geotrichum, Hansenula, Kluyveromyces, Pachysolen, Pichia, Rhodosporidium, Rhodotorula, Saccharomyces, Saccharomycodes, Saccharomycopis, Schizosaccharomyces and Zygosaccharomyces. Variations occurred in the number of bands and their positions in the gel, not only among strains of different genera but also among species from the same genus and even between varieties of the same species. The ascomycetous yeasts, with the exeption of Saccharomyces cerevisiae, only showed one to five bands of DNA larger than 1000 kilobase pairs (kb) in general none smaller. The paterns of the four basidiomycetous yeasts revealed also a few large DNA bands but in addition one to six bands ranging in size from 500 to 1000 kb, with the exception of a single smaller chromosome in Rhodotorula mucilaginosa. From the OFAGE banding patterns of strains studied here it appears that in Saach. cerevisiae the partitioning of DNA over chromosomes in unique. But rather than the large number of chromosomes, the presence of four chromosomes with less than 500 kb of DNA is characteristic for Sacch. cerevisiae.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020307
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Movements of intracellular particles in undifferentiated amebae of Dictyostelium discoideum (1987)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 7 (1987), S. 258-271 
    Details
    ISSN: 0886-1544
    Keywords: video and fluorescence microscopy ; saltatory particle movements ; cytoskeleton ; microtubules ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We recorded live, undifferentiated amebae of Dictynstelium discoideum by video microscopy and analyzed the behavior of cytoplasmic particles and granules. Cytoplasmic streaming and saltatory movements are the two major types of particle movements that occur in interphase amebae. Saltatory movements predominated in an area around the nucleus-associated body (NAB) and many were radial toward or away from it, the velocity being very similar in both directions. Some saltations were simple forward movements, and others were complex to-and-fro movements with as many as seven turnabouts. For a given leg of movement the velocity was not uniform along the path. Small particles (〈 1 μm) moved faster (X = 2.8 μm/s) than large (∼ 1 μm; X = 2.1 μm/s) and very large (〉 1 μm; X = 1.4 μm/s) particles, but the smallest particles were visible only in the running image and could not be analyzed. Ultrastructurally, saltating particles are digestive vacuoles and vesicles of various sizes, appearances, and contents, which are numerous particularly in the vicinity of the NAB. Several lines of evidence pointed to a role of microtubules (MTs) in saltatory particle movements. Composites of particle tracks corresponded closely to MT arrays visualized by immunofluorescence. No saltations occurred in mitotic amebae that lack cytoplasmic MTs, but the movements resumed toward the end of division, concurreduced with the rebuilding of the complex of cytoplasmic MTs. Nocodazole reduced and eventually slopped saltatory movements over a period of 3 h, when aberrant MT patterns were the rule. Saltations in slime mold amebae may be an eye-catching feature of intracellular transport functioning in endo- and exocytosis in the shuffling of vesicles containing factors involved in ameboid movement, and in the transduction of external signals to the cell center.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970070308
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Organization and dynamics of a cortical fibrous network of Paramecium: The outer lattice (1987)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 7 (1987), S. 315-324 
    Details
    ISSN: 0886-1544
    Keywords: cytoskeleton ; cell morphogenesis ; immunofluorescence ; antimyosin monoclonal antibody ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A monoclonal antibody, CC212, raised against ciliated cortices of quail oviduct cells and characterized as an antimyosin of smooth muscle and nonmuscle cells, was shown to specifically label a regular cortical network in Paramecium and to recognize two Triton X-100-insoluble polypeptides at 130 and 50 kDa. However, no evidence was obtained that these polypeptides are related to myosin.An immunofluorescence study and ultrastructural immunogold localization allowed us to identify the CC212-decorated material as a component of the outer lattice, a submembrane cytoskeletal network which runs along the top of the ridges visible by scanning electron microscopy and delineates the periphery of each cortical unit. The dynamics of the outer lattice during the cell cycle was studied by immunofluorescence and it was found that the outer lattice growth is achieved without disruption of the preexisting meshes by longitudinal elongation and additon of new transverse partitions. A striking disorganization of the outer lattice was observed in a thermosensitive mutant primarily altered in basal body duplication. These observations suggest possible functions of the outer lattice and demonstrate the interdependence of basal body duplication, surface growth, and outer lattice remodelling.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970070404
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Gene targeting and cell motility (1989)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 14 (1989), S. 62-68 
    Details
    ISSN: 0886-1544
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970140113
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Cell shape and organization of F-actin and microtubules in randomly moving and stationary amebae of Dictyostelium discoideum (1986)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 6 (1986), S. 176-185 
    Details
    ISSN: 0886-1544
    Keywords: Dictyostelium discoideum ; video and fluorescence microscopy ; random ameboid movement ; stationary mitotic amebae ; cytoskeleton ; microtubule center ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We investigated, by video-light microscopy and fluorescence microscopy with probes specific for microtribules (MTs) and F-actin, the relationship between cytoskeletal elements, cell shape and behavior of vegetative, undifferentiated amebae of Dictyostelium discoideum, strain NC-4. In an unconstrained situation, as on the underside of a coverglass in a thin layer of liquid medium, interphase cells moved around randomly in a polypodial or monopodial fashion. Locomotion was characterized by the formation of pseudodigits, rounded or pointed pseudopodia, and retraction fibers. F-actin occurred in all these structures, as well as in a thin cortical layer. Microtubules extended into some of the cellular extensions rich in F-actin. Pseudopodial activity, but not locomotion, also took place at the interface between medium and air, demonstrating that ameboid movement requires contact with a solid substrate. Stationary mitotic amebae on glass were studded with continuously changing, peripheral spike-shaped filopodia that also contained F-actin. During telophase and cytokinesis the spikes were gradually replaced by pseudopodia in transition to the fully motile phase.In live cells, the nucleus-associated body (NAB), which is at the center of the complex of cytoplasmic MTs [CMTC; term from Brinkley, Fuller, and Highfield, 1975] was in a rather fixed position; it did not orient in a concerted fashion to follow changes in the direction of cell movement. In amebae fixed and processed for fluorescence microscopy after a period of recorded movement, the NAB was not preferentially positioned with respect to the nucleus and the direction of movement. It is unlikely that the NAB exerts a directional control during randon ameboid movement. The complex of cytoskeletal MTs must be very dynamic or flexible to adjust to the rapid changes of cell shape.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970060216
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...