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  • 1
    Electronic Resource
    Electronic Resource
    Are centromeric dots kinetochores? (1975)
    [s.l.] : Nature Publishing Group
    Nature 254 (1975), S. 463-463 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] EiBERG1 described a Giemsa staining technique which reveals specific paired dots in the centromere region of human chromosomes in cells treated with colce-mid and hypotonic KCI. He suggested that the dots may represent organelles associated with spindle fibres. Evans and Ross2 demonstrated similar, ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/254463a0
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  • 2
    Electronic Resource
    Electronic Resource
    Indirect immunofluorescence of microtubules in Dictyostelium discoideum - A study with polyclonal and monoclonal antibodies to tubulins
    Amsterdam : Elsevier
    Experimental Cell Research 151 (1984), S. 183-193 
    Details
    ISSN: 0014-4827
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0014-4827(84)90367-7
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  • 3
    Electronic Resource
    Electronic Resource
    Tubulins of cellular slime molds analysed by SDS-PAGE and immunoblotting
    Amsterdam : Elsevier
    Cell Biology International Reports 14 (1990), S. 202 
    Details
    ISSN: 0309-1651
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0309-1651(90)90910-Q
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  • 4
    Electronic Resource
    Electronic Resource
    Cytoskeletal proteins in a fraction of nuclei from Dictyostelium amebae
    Amsterdam : Elsevier
    Cell Biology International Reports 14 (1990), S. 83 
    Details
    ISSN: 0309-1651
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0309-1651(90)90432-X
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  • 5
    Electronic Resource
    Electronic Resource
    Hyphal tip organization inAllomyces arbuscula (1977)
    Springer
    Protoplasma 93 (1977), S. 231-247 
    Details
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Light and electron microscopic observations on vegetative hyphae ofAllomyces arbuscula revealed the specialized organization of the tip. There were some minor differences related to culture conditions, but the main ultrastructural features common to all hyphal tips disclosed a special type of organization distinct from that of other fungi. A crescent-shaped apical zone consisted of vesicles and membrane cisternae embedded in a granular matrix. Vesicles fused with the apical plasmalemma and presumably contributed to its expansion and to wall growth. The apical zone contained few ribosomes and generally no other organelles. Mitochondria were concentrated in the immediate subapical zone and scattered through the remainder of the hyphae, as were microbodies. Microtubules formed an asterlike structure with its center in the apical zone. Proximally of the apex, microtubules were axially oriented. Nuclei occurred only a certain distance from the tip. The elements of the apex may maintain the polarity of the hyphae via a gradient and hold it in a state of vegetative growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01275656
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  • 6
    Electronic Resource
    Electronic Resource
    From proto-mitosis to mitosis — An alternative hypothesis on the origin and evolution of the mitotic spindle (1984)
    Springer
    Origins of life and evolution of the biospheres 13 (1984), S. 183-193 
    Details
    ISSN: 1573-0875
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract Based on the assumption that the ancestral proto-eukaryote evolved from an ameboid prokarybte I propose the hypothesis that nuclear division of the proto-eukaryote was effected by the same system of contractile filaments it used for ameboid movement and cytosis. When the nuclear membranes evolved from the cell membrane, contractile filaments remained associated with them. The attachment site of the genome in the nuclear envelope was linked to the cell membrane by specialized contractile filaments. During protomitosis, i.e., nuclear and cell division of the proto-eukaryote, these filaments performed segregation of the chromosomes, whereas others constricted and cleaved the nucleus and the mother cell. When microtubules (MTs) had evolved in the cytoplasm, they also became engaged in nuclear division. Initially, an extranuolear bundle of MTs assisted chromosome segregation by establishing a defined axis. The evolutionary tendency then was towards an increasingly important role for MTs. Spindle pole bodies (SPBs) developed from the chromosomal attachment sites in the nuclear envelope and organized an extranuclear central spindle. The chromosomes remained attached to the SPBs during nuclear division. In a subsequent step the spindle became permanently lodged inside the nucleus. Chromosomes detached from the SPBs and acquired kinetochores and kinetochore-MTs. At first, this spindle segregated chromosomes by elongation, the kinetochore-MTs playing the role of static anchors. Later, spindle elongation was supplemented by poleward movement of the chromosomes. When dissolution of the nuclear envelope at the beginning of mitosis became a permanent feature, the open spindle of higher eukaryotes was born.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00927170
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  • 7
    Electronic Resource
    Electronic Resource
    Movements of intracellular particles in undifferentiated amebae of Dictyostelium discoideum (1987)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 7 (1987), S. 258-271 
    Details
    ISSN: 0886-1544
    Keywords: video and fluorescence microscopy ; saltatory particle movements ; cytoskeleton ; microtubules ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We recorded live, undifferentiated amebae of Dictynstelium discoideum by video microscopy and analyzed the behavior of cytoplasmic particles and granules. Cytoplasmic streaming and saltatory movements are the two major types of particle movements that occur in interphase amebae. Saltatory movements predominated in an area around the nucleus-associated body (NAB) and many were radial toward or away from it, the velocity being very similar in both directions. Some saltations were simple forward movements, and others were complex to-and-fro movements with as many as seven turnabouts. For a given leg of movement the velocity was not uniform along the path. Small particles (〈 1 μm) moved faster (X = 2.8 μm/s) than large (∼ 1 μm; X = 2.1 μm/s) and very large (〉 1 μm; X = 1.4 μm/s) particles, but the smallest particles were visible only in the running image and could not be analyzed. Ultrastructurally, saltating particles are digestive vacuoles and vesicles of various sizes, appearances, and contents, which are numerous particularly in the vicinity of the NAB. Several lines of evidence pointed to a role of microtubules (MTs) in saltatory particle movements. Composites of particle tracks corresponded closely to MT arrays visualized by immunofluorescence. No saltations occurred in mitotic amebae that lack cytoplasmic MTs, but the movements resumed toward the end of division, concurreduced with the rebuilding of the complex of cytoplasmic MTs. Nocodazole reduced and eventually slopped saltatory movements over a period of 3 h, when aberrant MT patterns were the rule. Saltations in slime mold amebae may be an eye-catching feature of intracellular transport functioning in endo- and exocytosis in the shuffling of vesicles containing factors involved in ameboid movement, and in the transduction of external signals to the cell center.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970070308
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  • 8
    Electronic Resource
    Electronic Resource
    Cell shape and organization of F-actin and microtubules in randomly moving and stationary amebae of Dictyostelium discoideum (1986)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 6 (1986), S. 176-185 
    Details
    ISSN: 0886-1544
    Keywords: Dictyostelium discoideum ; video and fluorescence microscopy ; random ameboid movement ; stationary mitotic amebae ; cytoskeleton ; microtubule center ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We investigated, by video-light microscopy and fluorescence microscopy with probes specific for microtribules (MTs) and F-actin, the relationship between cytoskeletal elements, cell shape and behavior of vegetative, undifferentiated amebae of Dictyostelium discoideum, strain NC-4. In an unconstrained situation, as on the underside of a coverglass in a thin layer of liquid medium, interphase cells moved around randomly in a polypodial or monopodial fashion. Locomotion was characterized by the formation of pseudodigits, rounded or pointed pseudopodia, and retraction fibers. F-actin occurred in all these structures, as well as in a thin cortical layer. Microtubules extended into some of the cellular extensions rich in F-actin. Pseudopodial activity, but not locomotion, also took place at the interface between medium and air, demonstrating that ameboid movement requires contact with a solid substrate. Stationary mitotic amebae on glass were studded with continuously changing, peripheral spike-shaped filopodia that also contained F-actin. During telophase and cytokinesis the spikes were gradually replaced by pseudopodia in transition to the fully motile phase.In live cells, the nucleus-associated body (NAB), which is at the center of the complex of cytoplasmic MTs [CMTC; term from Brinkley, Fuller, and Highfield, 1975] was in a rather fixed position; it did not orient in a concerted fashion to follow changes in the direction of cell movement. In amebae fixed and processed for fluorescence microscopy after a period of recorded movement, the NAB was not preferentially positioned with respect to the nucleus and the direction of movement. It is unlikely that the NAB exerts a directional control during randon ameboid movement. The complex of cytoskeletal MTs must be very dynamic or flexible to adjust to the rapid changes of cell shape.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970060216
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  • 9
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    From proto-mitosis to mitosis ? An alternative hypothesis on the origin and evolution of the mitotic spindle (1984)
    Springer
    Details
    Publication Date: 1984-03-01
    Print ISSN: 0169-6149
    Electronic ISSN: 1573-0875
    Topics: Biology , Geosciences
    Published by Springer
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  • 10
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    Are centromeric dots kinetochores? (1975)
    Springer Nature
    Details
    Publication Date: 1975-04-01
    Print ISSN: 0028-0836
    Electronic ISSN: 1476-4687
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Published by Springer Nature
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