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  • American Association for the Advancement of Science (AAAS)
  • 1985-1989  (8)
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  • 1
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 1989-03-10
    Description: Tension and intracellular free calcium concentration [( Ca2+]i) were measured simultaneously in single smooth muscle cells isolated from the anterior byssus retractor muscle (ABRM) of Mytilus edulis that were loaded with the fluorescent Ca2+ indicator fura-2. Electrical stimulation evoked a transient elevation of [Ca2+]i associated with a "catch" contraction. During the catch state, however, [Ca2+]i was effectively at its resting level and was unaffected by 5-hydroxytryptamine, which induced a rapid relaxation from catch. The results indicate that a maintained high [Ca2+]i is not required for the maintenance of catch tension in intact ABRM and that there was no significant change in [Ca2+]i upon abolition of catch.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ishii, N -- Simpson, A W -- Ashley, C C -- Wellcome Trust/United Kingdom -- New York, N.Y. -- Science. 1989 Mar 10;243(4896):1367-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Zoological Institute, Faculty of Science, University of Tokyo, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2922614" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Benzofurans ; Bivalvia ; Calcium/*physiology ; Fluorescent Dyes ; Fura-2 ; In Vitro Techniques ; *Muscle Contraction ; Muscle, Smooth/*physiology ; Spectrometry, Fluorescence/instrumentation/methods
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 2
    Publication Date: 1989-12-15
    Description: The Voyager 2 encounter with the Neptune system included radio science investigations of the masses and densities of Neptune and Triton, the low-order gravitational harmonics of Neptune, the vertical structures of the atmospheres and ionospheres of Neptune and Triton, the composition of the atmosphere of Neptune, and characteristics of ring material. Demanding experimental requirements were met successfully, and study of the large store of collected data has begun. The initial search of the data revealed no detectable effects of ring material with optical depth tau [unknown] 0.01. Preliminary representative results include the following: 1.0243 x 10(26) and 2.141 x 10(22) kilograms for the masses of Neptune and Triton; 1640 and 2054 kilograms per cubic meter for their respective densities; 1355 +/- 7 kilometers, provisionally, for the radius of Triton; and J(2) = 3411 +/- 10(x 10(-6)) and J(4) = -26(+12)(-20)(x10(-6)) for Neptune's gravity field (J〉(2) and J(4) are harmonic coefficients of the gravity field). The equatorial and polar radii of Neptune are 24,764 +/- 20 and 24,340 +/- 30 kllometers, respectively, at the 10(5)-pascal (1 bar) pressure level. Neptune's atmosphere was probed to a pressure level of about 5 x 10(5) pascals, and effects of a methane cloud region and probable ammonia absorption below the cloud are evident in the data. Results for the mixing ratios of helium and ammonia are still being investigated; the methane abundance below the clouds is at least 1 percent by volume. Derived temperature-pressure profiles to 1.2 x 10(5) pascals and 78 kelvins (K) show a lapse rate corresponding to "frozen" equilibrium of the para- and ortho-hydrogen states. Neptune's ionosphere exhibits an extended topside at a temperature of 950 +/- 160 K if H(+) is the dominant ion, and narrow ionization layers of the type previously seen at the other three giant planets. Triton has a dense ionosphere with a peak electron concentration of 46 x 10(9) per cubic meter at an altitude of 340 kilometers measured during occultation egress. Its topside plasma temperature is about 80 +/- 16 K if N(2)(+) is the principal ion. The tenuous neutral atmosphere of Triton produced distinct signatures in the occultation data; however, the accuracy of the measurements is limited by uncertainties in the frequency of the spacecraft reference oscillator. Preliminary values for the surface pressure of 1.6 +/- 0.3 pascals and an equivalent isothermal temperature of 48 +/- 5 K are suggested, on the assumption that molecular nitrogen dominates the atmosphere. The radio data may be showing the effects of a thermal inversion near the surface; this and other evidence imply that the Triton atmosphere is controlled by vapor-pressure equilibrium with surface ices, at a temperature of 38 K and a methane mixing ratio of about 10(-4).〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Tyler, G L -- Sweetnam, D N -- Anderson, J D -- Borutzki, S E -- Campbell, J K -- Eshleman, V R -- Gresh, D L -- Gurrola, E M -- Hinson, D P -- Kawashima, N -- Kursinski, E R -- Levy, G S -- Lindal, G F -- Lyons, J R -- Marouf, E A -- Rosen, P A -- Simpson, R A -- Wood, G E -- New York, N.Y. -- Science. 1989 Dec 15;246(4936):1466-73.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17756001" target="_blank"〉PubMed〈/a〉
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    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 3
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 1989-03-03
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Simpson, R T -- New York, N.Y. -- Science. 1989 Mar 3;243(4895):1220.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17799904" target="_blank"〉PubMed〈/a〉
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 4
    Publication Date: 1986-07-04
    Description: Voyager 2 radio occultation measurements of the Uranian atmosphere were obtained between 2 and 7 degrees south latitude. Initial atmospheric temperature profiles extend from pressures of 10 to 900 millibars over a height range of about 100 kilometers. Comparison of radio and infrared results yields mole fractions near the tropopause of 0.85 and 0.15 +/- 0.05 for molecular hydrogen and helium, respectively, if no other components are present; for this composition the tropopause is at about 52 kelvins and 110 millibars. Distinctive features in the signal intensity measurements for pressures above 900 millibars strongly favor model atmospheres that include a cloud deck of methane ice. Modeling of the intensity measurements for the cloud region and below indicates that the cloud base is near 1,300 millibars and 81 kelvins and yields an initial methane mole fraction of about 0.02 for the deep atmosphere. Scintillations in signal intensity indicate small-scale stucture throughout the stratosphere and upper troposphere. As judged from data obtained during occultation ingress, the ionosphere consists of a multilayer structure that includes two distinct layers at 2,000 and 3,500 kilometers above the 100-millibar level and an extended topside that may reach altitudes of 10,000 kilometers or more. Occultation measurements of the nine previously known rings at wavelengths of 3.6 and 13 centimeters show characteristic values of optical depth between about 0.8 and 8; the maxim value occurs in the outer region of the in ring, near its periapsis. Forward-scattered signals from this ring have properties that differ from those of any of Saturn's rings, and they are inconsistent with a discrete scattering object or local (three-dimensional) assemblies of orbiting objects. These signals suggest a new kdnd of planetary ring feature characterized by highly ordered cylindrical substructures of radial scale on the order of meters and azimuthal scale of kilometers or more. From radio data alone the mass of the Uranian system is GM(sys) = 5,794,547- 60 cubic kilometers per square second; from a combination of radio and optical navigation data the mass of Uranus alone is GM(u) = 5,793,939+/- 60 cubic kilometers per square second. From all available Voyager data, induding imaging radii, the mean uncompressed density of the five major satellites is 1.40+/- 0.07 grams per cubic centimeter; this value is consistent with a solar mix of material and apparently rules out a cometary origin of the satellites.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Tyler, G L -- Sweetnam, D N -- Anderson, J D -- Campbell, J K -- Eshleman, V R -- Hinson, D P -- Levy, G S -- Lindal, G F -- Marouf, E A -- Simpson, R A -- New York, N.Y. -- Science. 1986 Jul 4;233(4759):79-84.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17812893" target="_blank"〉PubMed〈/a〉
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 5
    Publication Date: 1986-12-05
    Description: Cortisol production requires the activity of only 17 alpha-hydroxylase, whereas the formation of sex steroids requires both 17 alpha-hydroxylase and 17,20-lyase activities. Studies in reconstituted enzyme systems have suggested that a single steroid hydroxylase, 17 alpha-hydroxylase cytochrome P-450 (P-450(17) alpha), catalyzes both activities. By expression of bovine adrenocortical P-450(17 alpha) in COS 1 (transformed monkey kidney) cells, which normally contain no detectable P-450(17) alpha, it has now been established in situ that a single polypeptide chain does catalyze both the 17 alpha-hydroxylase and the 17,20-lyase reactions. This heterologous system supports 17 alpha-hydroxylation of pregnenolone and progesterone with equal efficiency, but catalyzes about five times as much 17,20-lyase activity when 17 alpha-hydroxypregnenolone is the substrate than when 17 alpha-hydroxyprogesterone is the substrate. For these activities to be observed in COS 1 cells, newly synthesized apocytochrome P-450(17) alpha must bind heme and insert into the endoplasmic reticulum such that endogenous cytochrome P-450 reductase can support hydroxylation. Thus, COS 1 cells are a useful system for expression and study of various forms of cytochrome P-450.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Zuber, M X -- Simpson, E R -- Waterman, M R -- 5-T 32HD07190/HD/NICHD NIH HHS/ -- AM 238350/AM/NIADDK NIH HHS/ -- HD 13234/HD/NICHD NIH HHS/ -- New York, N.Y. -- Science. 1986 Dec 5;234(4781):1258-61.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3535074" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cattle ; Cercopithecus aethiops ; Cytochrome P-450 Enzyme System/genetics/*metabolism ; DNA/*metabolism ; Fluorescent Antibody Technique ; Kidney/cytology ; Multienzyme Complexes/genetics/metabolism ; Pregnenolone/metabolism ; Progesterone/metabolism ; Steroid 17-alpha-Hydroxylase/*metabolism ; Steroid Hydroxylases/genetics/*metabolism
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    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 6
    Publication Date: 1985-02-08
    Description: To show that honey bees are effective biological monitors of environmental contaminants over large geographic areas, beekeepers of Puget Sound, Washington, collected pollen and bees for chemical analysis. From these data, kriging maps of arsenic, cadmium, and fluoride were generated. Results, based on actual concentrations of contaminants in bee tissues, show that the greatest concentrations of contaminants occur close to Commencement Bay and that honey bees are effective as large-scale monitors.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bromenshenk, J J -- Carlson, S R -- Simpson, J C -- Thomas, J M -- New York, N.Y. -- Science. 1985 Feb 8;227(4687):632-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17781823" target="_blank"〉PubMed〈/a〉
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  • 7
    Publication Date: 1985-11-08
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Simpson, R L -- New York, N.Y. -- Science. 1985 Nov 8;230(4726):658-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17797287" target="_blank"〉PubMed〈/a〉
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  • 8
    Publication Date: 1986-07-04
    Description: The endogenous small subunit of the ribulose-1,5-bisphosphate carboxylase gene rbcS and the light-harvesting chlorophyll a/b-binding protein gene (LHCP) of pea are expressed in a light-inducible manner and are active mainly in green chloroplast-containing tissue. Chimeric genes under control of the 5'-flanking sequences of the rbcS ss3.6 or LHCP AB80 genes from pea were used to study the factors relating to the issue-specific and lightinducible expression of these nuclear-encoded genes in transgenic tobacco plants. The results show that plastid development plays a crucial role in the activation of expression of these chimeric genes. Particular members of each of the above gene families respond differently to tissue-specific and environmental factors. Furthermore, the light-inducible expression directed by the 5'-flanking sequence of ss3.6 rbcSgene is not exclusively mediated by phytochrome, but probably is controlledin large part by another photoreceptor.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Simpson, J -- VAN Montagu, M -- Herrera-Estrella, L -- New York, N.Y. -- Science. 1986 Jul 4;233(4759):34-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17812887" target="_blank"〉PubMed〈/a〉
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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