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  • 1
    Electronic Resource
    Electronic Resource
    Transient electric field generated by nonequilibrium states in superconducting Pb films (1991)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 59 (1991), S. 3333-3335 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Nonequilibrium states can be excited in superconducting Pb films with laser pulses of picosecond duration. We used the technique of electro-optic sampling to observe the transient electric field generated by these states in the presence of a supercurrent. It follows from our experiment that the switching between the superconducting and normal states occurs within 1 ps after the laser excitation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.105722
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  • 2
    Electronic Resource
    Electronic Resource
    The temperature dependence of the resistive response of superconducting Pb films upon picosecond optical excitation (1991)
    Springer
    Applied physics 52 (1991), S. 155-159 
    Details
    ISSN: 1432-0630
    Keywords: 74.30.Ga ; 73.60.Ka
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract We report a study of the temperature dependence of the transient resistivity of superconducting Pb thin films excited by short duration (∼70 ps) laser pulses of varying irradiances. The results are analyzed in terms of a model proposed by Elesin which features the existence of a nonstationary, inhomogeneous, intermediate state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00323734
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  • 3
    Electronic Resource
    Electronic Resource
    A study about pH chemically modified electrodes based on amino-derivatives of naphthalene (1992)
    Springer
    Microchimica acta 107 (1992), S. 27-36 
    Details
    ISSN: 1436-5073
    Keywords: chemically modified electrode ; pH sensor ; poly(1-naphthylamine) ; poly(2,3-diaminonaphthalene)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The electrochemical polymerization of amino-derivatives of naphthalene has been studied on the platinum wire electrodes. The effects of acidity of the modifying media and the potential scan rate on the cyclic voltammograms are verified. As potentiometric pH sensors, the electrodes prepared from 1-naphthylamine and 2,3-diaminonaphthalene showed performance characteristics superior to some other electrodes tested. The electrode modified with 1-naphthylamine in the optimum medium showed a nearly Nernstian response of 4.20–13.70 pH and a slope of -54.8 mV/pH, while the linear range of the electrode prepared by 2,3-diaminonaphthalene was 4.00–13.60 pH, with a slope of -52.4mV/pH.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01772350
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  • 4
    Electronic Resource
    Electronic Resource
    Purification of outer membrane iron transport receptors from Escherichia coli by fast protein liquid chromatography: FepA and FecA (1993)
    Springer
    BioMetals 6 (1993), S. 25-35 
    Details
    ISSN: 1572-8773
    Keywords: binding competition ; ferric enterobactin receptor protein (FepA) ; ferric citrate receptor protein (FecA) ; iron transport receptors ; purification of membrane proteins ; specificity of binding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Fast protein liquid chromatography (FPLC) with DEAE-Sepharose Fast Flow, PBE-94 and Q-Sepharose Fast Flow columns are applied to the purification of the ferric enterobactin protein receptor (FepA). The apparent single band of FepA on SDS-PAGE is isolated and purified into two proteins with very similar molecular weights. The two proteins are identified to be FepA and ferric citrate protein receptor (FecA) by N-terminus amino acid determination and a computer search with the Gene Bank file. The assay of binding activities of these proteins shows that both FepA and FecA bind ferric enterobactin, with the former having about double the activity of the latter. Competition studies shows that Fe-MECAM is competitively bound to both proteins and that ferric parabactin only slightly competes with [55Fe]ferric enterobactin. It is found that ferrichrome A has no effect on the binding of the receptor proteins with ferric enterobactin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00154229
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  • 5
    Electronic Resource
    Electronic Resource
    Molecular identification of a soybean protein kinase gene family by using PCR (1992)
    Springer
    Plant molecular biology 18 (1992), S. 581-584 
    Details
    ISSN: 1573-5028
    Keywords: gene family ; polymerase chain reaction ; protein kinases ; signal transduction ; soybean (Glycine max L.)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In this study we report identification of six members of a protein kinase gene family from soybean (Glycine max L.). Two fully degenerate oligonucleotide primers corresponding to two conserved motifs (DLK-PENV and GTHEYLAPE) in the catalytic domains of eukaryotic protein serine/threonine kinases were used in a polymerase chain reaction (PCR) to amplify soybean cDNA. Sequence analysis showed that 28 of the PCR sequences represented six different putative protein serine/threonine kinases. These results not only demonstrate that catalytic domains of protein kinases are highly conserved between plants and other eukaryotes but also suggest that there are multiple genes encoding protein kinases in plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040673
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  • 6
    Electronic Resource
    Electronic Resource
    Molecular cloning and biochemical characterization of a receptor-like serine/threonine kinase from rice (1994)
    Springer
    Plant molecular biology 26 (1994), S. 791-803 
    Details
    ISSN: 1573-5028
    Keywords: ATP ; GTP ; protein kinase ; receptor ; rice ; signal transduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A receptor-like protein kinase, OsPK10, has been cloned from rice (Oryza sativa). The 2.8 kb cDNA contains an open reading frame capable of encoding a peptide sequence of 824 amino acids. The topological features of the predicted OsPK10 protein include an N-terminal signal peptide, a cysteine-rich extracellular ligand-binding domain, a membrane-spanning segment, and a cytoplasmic domain possessing all the hallmarks of catalytic domains of eukaryotic protein kinases. The cytoplasmic domain was selectively expressed in Escherichia coli and assayed for kinase activity. The results show the protein is capable of autophosphorylation using either ATP or GTP as the phosphate donor. Phosphoamino acid analysis reveals phosphorylation of threonines, consistent with the substrate specificity indicated by sequence motifs in the catalytic core. A single amino acid substitution of Glu for Lys-528 completely abolishes autophosphorylation activity. DNA gel blot analyses suggest that the haploid rice genome contains a single copy of the OsPK10 gene. OsPK10 transcripts appear to be more abundant in shoots than in roots of rice seedlings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00028849
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  • 7
    Electronic Resource
    Electronic Resource
    Restoration of shooty morphology of a nontumorous mutant of Nicotiana glauca x N. langsdorffii by cytokinin and the isopentenyltransferase gene (1990)
    Springer
    Plant molecular biology 15 (1990), S. 407-420 
    Details
    ISSN: 1573-5028
    Keywords: cytokinin ; tumor formation ; isopentenyltransferase gene ; Nicotiana ; genetic tumors ; polymerase chain reaction (PCR) ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The shooty morphology of a nontumorous amphidiploid mutant of Nicotiana glauca Grah. x N. langsdorffii Weinm. was restored by cytokinins, whether exogenously applied or endogenously produced by transformation of the mutant with a transfer DNA (T-DNA) cytokinin-biosynthesis gene (isopentenyltransferase; ipt). Auxins alone did not confer this effect. Similar transformation was not achieved for the parental species. In the case of transformation with the ipt gene, selection of the transformed tissues was based on its hormone-independent growth in the presence of the antibiotic kanamycin. Transformed tissues exhibited a shooty morphology, indistinguishable from that of wildtype genetic tumors N. glauca x N. langsdorffii. This altered phenotype was caused by the presence and constitutive expression of the ipt gene. The insertion and expression of this gene in transformed tissues was confirmed by using the polymerase chain reaction (PCR) technique as well as conventional molecular hybridization analysis. Expression of the ipt gene led to an elevated level of cytokinin in the transformed mutant tissues. This evidence supports the notion that genetic tumors are caused, at least in part, by elevated levels of cytokinin in interspecific hybrids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019158
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  • 8
    Electronic Resource
    Electronic Resource
    Artificial neural networks applied to potentiometric titration of multi-component polybasic acid mixtures (1993)
    Springer
    Microchimica acta 111 (1993), S. 199-206 
    Details
    ISSN: 1436-5073
    Keywords: chemometrics ; artificial neural network ; multi-component calibration ; potentiometric acid-base titration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A three-layer artificial neural network model with back-propagation of error is used to treat potentiometric acid-base titration data for estimating the concentrations of individual components in polybasic weak acid mixtures. The network's architecture and parameters were optimized and an empirical rule for dynamically adjusting the learning rate is put forward to improve the network's performance. Satisfactory prediction results were obtained for three-component samples containing maleic acid, propandioic acid and succinic acid with an average relative error of 4.5%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01245307
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  • 9
    Electronic Resource
    Electronic Resource
    A novel purification of ferric citrate receptor (FecA) from Escherichia coli UT5600 and further characterization of its binding activity (1993)
    Springer
    BioMetals 6 (1993), S. 37-44 
    Details
    ISSN: 1572-8773
    Keywords: binding activity ; ferric citrate binding protein ; FPLC chromatofocusing ; iron uptake ; purification of membrane protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In our earlier paper, it was demonstrated that the FecA receptor protein from Escherichia coli UT5600/pBB2 (leu −, proC −, trpE −, entA −, rpsl −, Δ(ompT-fepA)−/Ampr, fepA) binds with ferric enterobactin. In order to explore this further the outer membrane receptor protein, FecA, has been isolated from UT5600 (fepA −) and purified to homogeneity by DE-52-cellulose anion exchange chromatography followed by MonoPFPLC chromatofocusing. Partially purified FecA and homogeneous FecA show binding activity to [55Fe]ferric enterobactin and the binding is specific. Binding activity of FecA can be enhanced by ferric citrate. Lipopolysaccharide-free FecA as ascertained by silver staining and the endotoxin test still retains the same activity. In vivo uptake studies using different strains of E. coli suggest that FecA in E. coli plays an important role in ferrienterobactin transport.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00154230
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  • 10
    Electronic Resource
    Electronic Resource
    Robust principal component analysis by projection pursuit (1993)
    New York, NY : Wiley-Blackwell
    Journal of Chemometrics 7 (1993), S. 527-541 
    Details
    ISSN: 0886-9383
    Keywords: Principal component analysis ; Projection pursuit ; Simulated annealing algorithm ; Robust statistics ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Principal component analysis (PCA) is a widely used technique in chemometrics. The classical PCA method is, unfortunately, non-robust, since the variance is adopted as the objective function. In this paper, projection pursuit (PP) is used to carry out PCA with a criterion which is more robust than the variance. In addition, the generalized simulated annealing (GSA) algorithm is introduced as an optimization procedure in the process of PP calculation to guarantee the global optimum. The results for simulated data sets show that PCA via PP is resistant to the deviation of the error distribution from the normal one. The method is especially recommended for use in cases with possible outlier(s) existing in the data.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cem.1180070606
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