ISSN:
1618-2650
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary A fluorescent high-performance liquid chromatography (HPLC) analysis of folic acid (pteroylglutamic acid) and its derivatives using permanganate as a fluorogenic reagent has been developed. In the present work, the various analytical conditions in which the fluorogenic reaction with permanganate could be applied to the HPLC of folic acid in human serum, have been studied. The present method may be employed for the rapid clinical analysis of small amounts of folic acid (≥150 ppb), without interference from coexisting natural fluorophors; the precision of the present method for folic acid was 6.6% (n=6) (coefficient of variation).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00321302
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