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Fluorescent high-performance liquid chromatography of folic acid and its derivatives using permanganate as a fluorogenic reagent

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  • Biological/Clinical Materials
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Summary

A fluorescent high-performance liquid chromatography (HPLC) analysis of folic acid (pteroylglutamic acid) and its derivatives using permanganate as a fluorogenic reagent has been developed. In the present work, the various analytical conditions in which the fluorogenic reaction with permanganate could be applied to the HPLC of folic acid in human serum, have been studied. The present method may be employed for the rapid clinical analysis of small amounts of folic acid (≥150 ppb), without interference from coexisting natural fluorophors; the precision of the present method for folic acid was 6.6% (n=6) (coefficient of variation).

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Authors and Affiliations

  1. Department of Chemistry, Hamamatsu University School of Medicine, Handa-cho 3600, Hamamatsu-shi, 431-31, Shizuoka, Japan

    Norio Ichinose & Mikako Kato

  2. Shizuoka Institute of Science and Technology, Toyosawa 2200-2, Fukuroi-shi, 436, Shizuoka, Japan

    Toshihiro Tsuneyoshi

  3. Department of Pharmacy, Hamamatsu University School of Medicine, Japan

    Tokinori Suzuki

  4. Department of Material Chemistry, Faculty of Science and Technology, Ryukoku University, Setaoue-cho Yokoya 1-5, Outsu-shi, 520-21, shiga, Japan

    Shigero Ikeda

Authors
  1. Norio Ichinose
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  2. Toshihiro Tsuneyoshi
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  3. Mikako Kato
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  4. Tokinori Suzuki
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  5. Shigero Ikeda
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Dedicated to Professor Dr. Wilhelm Fresenius on the occasion of his 80th birthday

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Ichinose, N., Tsuneyoshi, T., Kato, M. et al. Fluorescent high-performance liquid chromatography of folic acid and its derivatives using permanganate as a fluorogenic reagent. Fresenius J Anal Chem 346, 841–846 (1993). https://doi.org/10.1007/BF00321302

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  • DOI: https://doi.org/10.1007/BF00321302

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