ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Water extraction of peanut proteins was optimized to maximize protein extraction, Y1 and protein concentration in the extract, Y2. A central composite design involving solids-to-water ratio (X1), pH (X2), temperature (X3) and time (X4) was used, and second-order models for Y1 and Y2 were employed to generate response surfaces. The optimum conditions to obtain Y1≥ 85% and Y2≥ 2.5% were X1= 1:8; X2= 8.0; X3= 50 °C; X4= 30 min. Estimates of Y1 and Y2 at the optimum were 85.29% and 2.7%, respectively. Experimental verification gave values of Y1= 84.39%± 0.78 and Y2= 2.80%± 0.02. Y1 and Y2 were further maximized, as measured by absorbance of solution following centrifugation of the extract, when papain (0.05% g/g peanuts) was added and extraction was carried out at the optimum conditions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1991.tb08665.x
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