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  • 1
    Electronic Resource
    Electronic Resource
    Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max) (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 83 (1991), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Inhibition of root elongation and modification of membrane properties are sensitive responses of plants to aluminium. The present paper reports on the effect of AI on lipid peroxidation and activities of enzymes related to production of activated oxygen species. Soybean seedlings (Glycine max L. cv. Sito) were precultured in solution culture for 3–5 days and then treated for 1–72 h with Al (AICI3) concentrations ranging from 10 to 75 μM at a constant pH of 4.1. In response to Al supply, lipid peroxidation in the root tips (〈 2 cm) was enhanced only after longer durations of treatment. Aluminium-dependent increase in lipid peroxidation was intensified by Fe2+ (FeSO4). A close relationship existed between lipid peroxidation and inhibition of root-elongation rate induced by Al and/or Fe toxicity and/or Ca deficiency. Besides enhancement of lipid peroxidation in the crude extracts of root tips due to Al, the activities of superoxide dismutase (EC 1.15.1.1) and peroxidase (EC 1.11.1.7) increased, whereas catalase (EC 1.11.1.6) activity decreased. This indicates a greater generation of oxygen free radicals and related tissue damage. The results suggest that lipid peroxidation is part of the overall expression of Al toxicity in roots and that enhanced lipid peroxidation by oxygen free radicals is a consequence of primary effects of Al on membrane structure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1991.tb00121.x
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  • 2
    Electronic Resource
    Electronic Resource
    Short-term effects of boron, germanium and high light intensity on membrane permeability in boron deficient leaves of sunflower (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 95 (1995), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The effects of varied boron (B) supply (0.01–50 μM and light intensity (100–580 μpmol m−2 s−1) on plant growth, leaf symptoms and membrane permeability of leaves were studied in sunflower plants over a 10-day-period of growth in nutrient solution. Membrane permeability was measured by incubating isolated young leaves in aerated distilled water. Additional experiments showed the effect of short-term supply (20 min to 2 h) of B and germanium (Ge) on membrane permeability of B-deficient leaves incubated in distilled water. Low supply of B decreased shoot and root growth and caused brown-purple pigmentation of young leaves, particularly under high light intensities. Leakage of K- from leaves increased with severity of B deficiency whereas, in B-sufficient leaves, leakage of solutes was low. Also leakage of phenolics, amino acids and sucrose was enhanced by B deficiency. Compared to B-sufficient leaves, the leakage from B-deficient leaves was 35-fold higher for K+, 45-fold higher for sucrose and 7-fold higher for phenolics and amino acids. Increases in solute leakage were marked in leaf areas with brown-purple pigmentation and particularly pronounced by increasing light intensity or by exposure of leaves to continuous light. In severely B-deficient leaves grown under high light intensity, treatment with B and Ge up to 1 000 μMM for 20 min resulted in an immediate decrease in K+ efflux to about the same levels as in B-sufficient leaves. Similar rapid decreases in K+ efflux were also found in B-deficient leaves when exposed to darkness for 16 h or 30 h before the leakage measurement.The results demonstrate a particular role for B in maintaining the integrity of plasma membranes. Boron presumably stabilizes the structure of the plasma membrane by complexing membrane constituents. It also has a protective effect on membrane constituents by complexing phenolics, so that oxidation of phenolics to highly toxic quinones and oxygen free radicals is prevented or limited. In these functions, boron seems to be to a large extent replaceable by germanium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1995.tb00801.x
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  • 3
    Electronic Resource
    Electronic Resource
    Mechanism of phosphorus-induced zinc deficiency in cotton. I. Zinc deficiency-enhanced uptake rate of phosphorus (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 68 (1986), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The effect of withholding Zn on the uptake, translocation and accumulation of P was studied in cotton plants (Gossypium hirsutum L. cv. Deltapine 15/21) grown in nutrient solutions under controlled environmental conditions. The influence of P on the uptake rate, translocation and distribution of 65Zn in the plants was also examined. Increasing the P supply resulted in severe Zn deficiency symptoms (interveinal chlorosis) as well as P toxicity symptoms, which were characterized by leaf puckering and grayish-brown marginal necrosis. Zinc deficiency markedly increased the uptake and translocation rates of P over the whole concentration range tested (5x10-5 to 1.25x10-3M). Uptake and translocation rates of P increased with both level of P and severity of Zn deficiency. This often caused P toxicity symptoms on Zn-deficient leaves. In contrast to P, the concentrations of K and Mg in the leaves were not affected by Zn deficiency. Similar results were obtained for sunflower (Helianthus annuus L.) and buckwheat (Fagopyrum esculentum Moench) plants. Higher P concentrations in Zn-deficient leaves or shoots could not be attributed wholly to reduced shoot growth. This was also evident when Zn deficiency was compared with other micronutrient (Fe, Mn, and Cu) deficiencies. Only Zn-deficient plants showed enhanced uptake and translocation of P. In experiments with 65Zn, a high P supply did not depress uptake and translocation of Zn. From the results obtained it is concluded that the P-induced Zn deficiency in cotton, as well as in other species, is primarily caused by enhanced P uptake and translocation and not by inhibition of Zn uptake.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1986.tb03386.x
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  • 4
    Electronic Resource
    Electronic Resource
    Mechanism of phosphorus-induced zinc deficiency in cotton. II. Evidence for impaired shoot control of phosphorus uptake and translocation under zinc deficiency (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 68 (1986), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Cotton (Gossypium hirsutum L. cv. Deltapine 15/21) plants were precultured for 19 to 25 days under controlled climatic conditions in nutrient solutions with different levels of Zn. With the onset of visual Zn-deficiency symptoms the pH of the nutrient solution decreased from 6.0 to about 5.0. In contrast, Zn-sufficient plants raised the pH of the nutrient solution to about 7.0. In short-term studies it could be demonstrated that the Zn nutritional status of the plants remarkably influenced the uptake and translocation rates of mineral nutrients. Compared to Zn-sufficient plants, P uptake rate in severely Zn-deficient plants was increased by a factor of 2 to 3, whereas the uptake rates of K, Ca and particularly NO3 decreased. The accumulation of P in the roots of Zn-deficient plants was either not affected or even lower than in Zn-sufficient plants. Thus, Zn deficiency had a specific enhancement effect on root to shoot transport of P. This enhancement effect of Zn deficiency on uptake and transport of P was similar at nutrient solution pH values of 7.0 and 5.8; i.e. it was not the result of acidification of the nutrient solution. After application of 36CI, 86Rb and 32P to plant stems, basipetal transport of 36CI and 86Rb was not affected by the Zn nutritional status of the plants. However, in Zn-deficient plants, only 7.8% of the 32P was translocated basipetally compared to 34% in the Zn-sufficient plants. A resupply of Zn for 19 h to Zn-deficient plants enhanced basipetal 32P transport. The results indicate that a feedback mechanism in the shoots is impaired in Zn-deficient plants which controls the P uptake by roots and especially the P transport from roots to shoots. As a result of this impairment toxic concentrations of P accumulate in the leaves. The mechanism responsible is likely the retranslocation of P in the phloem from shoots to roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1986.tb03387.x
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  • 5
    Electronic Resource
    Electronic Resource
    Zinc-dependent changes in ESR signals, NADPH oxidase and plasma membrane permeability in cotton roots (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 73 (1988), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The effect of Zn2+ on the plasma membrane permeability and superoxide radical (O2-) formation in roots was studied with cotton (Gossypium hirsutum L. cv. Delta-pine 15/21) plants grown in nutrient solution with different Zn2+ supply. Compared to Zn-sufficient plants, the plasma membrane permeability of Zn-deficient plants was increased as indicated by a 3-, 5- and 2.5-fold increase in root cell leakage of K+, NO3- and organic carbon compounds, respectively. Resupply of Zn2+ to Zn-deficient plants for 12 h substantially decreased this leakage. The effects of Zn2+ on membrane permeability were closely correlated with the levels of O2- measured by electron spin resonance (ESR) spectroscopy in the microsomal membrane fraction and in the cytosol fraction of root cells. The amplitudes of the O2- -derived Tiron ESR signal also coincided with a O2- -generating oxidase activity which was strongly dependent on the presence of NADPH and FAD. The results suggest that Zn2+ directly affects the integrity of the plasma membrane, at least in part, by interfering with O2- generation by a membrane-bound NADPH oxidase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1988.tb09214.x
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  • 6
    Electronic Resource
    Electronic Resource
    Mechanism of phosphorus-induced zinc deficiency in cotton. III. Changes in physiological availability of zinc in plants Is mail (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 70 (1987), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The effect of varied supply of P (2.5× 10−5 to 6× 10−4 M) and Zn (0 to 10−6 M) on uptake and concentrations of P and Zn was studied in cotton (Gossypium hirsutum L. cv. Deltapine 15/21) grown in nutrient solution under controlled environmental conditions. At a given Zn supply, increasing levels of P had no significant effect on the concentrations of total Zn in plants. However, increasing levels of P induced or enhanced visual Zn deficiency symptoms when the Zn concentration in the nutrient solution was low. The concentrations of water-soluble Zn in roots and shoots constituted 60% of the total Zn concentrations for plants grown with low P and 30% for plants grown with high P. The concentration of water-soluble Zn in leaves, but not total Zn, was closely correlated with visual Zn deficiency symptoms, levels of chlorophyll, super oxide dismutase and membrane permeability. The critical deficiency concentration of water-soluble Zn in cotton leaves was in the range of 6 to 7 μg (g dry weight)−1 or about 1.0 μg (g fresh weight)−1. The results show that high P concentrations in plant tissue decrease the physiological availability of Zn. Water-soluble Zn in the tissue appears to be a suitable indicator for Zn nutritional status in general and phosphorus-induced Zn deficiency in particular. Also in field-grown orange trees (Citrus sinensis) visual Zn deficiency symptoms in leaves were closely related to the concentration of water-soluble Zn.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1987.tb08690.x
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  • 7
    Electronic Resource
    Electronic Resource
    Decrease in nitrate uptake and increase in proton release in zinc deficient cotton, sunflower and buckwheat plants (1990)
    Springer
    Plant and soil 129 (1990), S. 261-268 
    Details
    ISSN: 1573-5036
    Keywords: cation-anion balance ; Fagopyrum esculentum ; Gossypium hirsutum ; Helianthus annuus ; nitrate efflux ; nitrate uptake ; pH decrease ; zinc deficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of varied Zn supply on the pH of the nutrient solution and uptake of cations and anions was studied in cotton (Gossypium hirsutum L.), sunflower (Helianthus annuus L.) and buckwheat (Fagopyrum esculentum Moench) plants grown under controlled environmental conditions in nutrient solutions with nitrate as source of nitrogen. With the appearance of visual Zn deficiency symtoms, the pH of the nutrient solutions decreased from 6 to about 5 whereas the pH increased to about 7 when the plants were adequately supplied with Zn. In Zn deficient plants the pH decrease was associated with a shift in the cation-anion uptake ratio in favour of cation uptake. Of the major ions, uptake of Ca2+ and K+ was either not affected or only slightly lowered whereas NO3 - uptake was drastically decreased in Zn deficient plants. Although the Zn nutritional status of plants hardly affected the NO3 - concentrations in the plants, the leakage of NO3 - from roots of Zn deficient plants into a diluted CaCl2 solution was nearly 10 times higher than that of plants adequately supplied with Zn. In contrast to Zn deficiency, Mn deficiency in cotton plants neither affected NO3 - uptake nor the pH of the nutrient solution. The results indicate that, probably as a consequence of the role of Zn in plasma membrane integrity and nitrogen metabolism, when Zn is deficient in dicotyledonous species net uptake of NO3 - is particularly depressed which in turn results in an increase in cation-anion uptake ratio and a corresponding decrease in external pH. The ecological relevance of this rhizosphere acidification is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00032421
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  • 8
    Electronic Resource
    Electronic Resource
    Boron deficiency-induced impairments of cellular functions in plants (1997)
    Springer
    Plant and soil 193 (1997), S. 71-83 
    Details
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The essentiality of B for growth and development of plants is well-known, but the primary functions of B still remain unknown. Evidence in the literature supports the idea that the major functions of B in growth and development of plants are based on its ability to form complexes with the compounds having cis-diol configurations. In this regard, the formation of B complexes with the constituents of cell walls and plasma membranes as well as with the phenolic compounds seems to be a decisive step affecting the physiological functions of B. Boron seems to be of crucial importance for the maintenance of structural integrity of plasma membranes. This function of B is mainly related to stabilisation of cell membranes by B association with membrane constituents. Possibly, B may also protect plasma membranes against peroxidative damage by toxic O2 species. In B-deficient plants, plasma membranes are highly leaky and lose their functional integrity. Under B-deficient conditions, substantial changes in ion fluxes and proton pumping activity of the plasma membranes were noted. Impairments in phenol metabolism and increases in levels of phenolics and polyphenoloxidase activity are typical indications of B deficiency, particularly in B deficiency-sensitive plant species, such as Helianthus annuus (sunflower). Enhanced oxidation of phenols is responsible for generation of reactive quinones which subsequently produce extremely toxic O2 species, thus resulting in the increased risk of a peroxidative damage to vital cell components such as membrane lipids and proteins. In B-deficient tissues, enhancement in levels of toxic O2 species may also occur as a result of impairments in photosynthesis and antioxidative defence systems. Recent evidence shows that the levels of ascorbic acid, non-protein SH-compounds (mainly glutathione) and glutathione reductase, the major defence systems of cells against toxic O2 species, are reduced in response to B deficiency. There is also increasing evidence that, in the heterocyst cells of cyanobacteria, B is involved in protection of nitrogenase activity against O2 damage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1004259808322
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  • 9
    Electronic Resource
    Electronic Resource
    Photopolymerization of styrene and methyl methacrylate using macro photoinitiators. Synthesis and characterization of PS-PEG-PS or PMMA-PEG-PMMA block copolymers (1995)
    Weinheim : Wiley-Blackwell
    Angewandte Makromolekulare Chemie 231 (1995), S. 123-133 
    Details
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Makrophotoinitiatoren aus Poly(ethylenglykol) (PEG) mit Benzoylxanthat-Segmenten wurden durch Reaktion der PEG-Xanthate mit Benzoylchlorid hergestellt. Diese Makrophotoinitiatoren mit PEGs unterschiedlicher Molekulargewichte wurden eingesetzt, um durch lichtinduzierte radikalische Polymerisation von Styrol und Methyl-methacrylat Blockcopolymere mit inneren PEG-Segmenten zu synthetisieren. Der Ein-fluß der Konzentration der Reaktanden und der Polymerisationszeit auf die Ausbeute und die Viskosität der Blockcopolymeren wurde untersucht. Die Blockcopolymeren wurden durch fraktionierte Fällung und IR-, UV- und 1H-NMR-Spektroskopie charakterisiert.
    Notes: Macro photoinitiators based on poly(ethylene glycol)s (PEG) bearing benzoyl xanthate moieties were synthesized by the reaction of PEG xanthates with benzoyl chloride. These macro photoinitiators possessing PEGs of different molecular weights were used in the photoinduced radical polymerization of styrene (S) and methyl methacrylate (MMA) to yield block copolymers having an inner-PEG segment. The influence of the concentration of reactants and polymerization time on the yield and intrinsic viscosities of block copolymers were also investigated. Characterization of block copolymers was carried out by fractional precipitation and IR, UV and 1H NMR spectroscopic methods.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/apmc.1995.052310111
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  • 10
    Electronic Resource
    Electronic Resource
    Preparation of multiphase block copolymers by redox polymerization process (1992)
    Weinheim : Wiley-Blackwell
    Angewandte Makromolekulare Chemie 195 (1992), S. 121-127 
    Details
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Durch Ce(IV)-initiierte Redoxypolymerisation von Methylmethacrylat mit Poly(oxyethylen), das Azo- und Hydroxygruppen enthielt, wurden Copolymere mit labilen Azogruppen in der Hauptkette erhalten. Diese Prepolymeren wurden zur durch thermischen Zerfall der Azogruppen initiierten radikalischen Polymerisation von Styrol eingesetzt, wobei Multiblockcopolymere entstanden, was durch fraktionierte Fällung, Änderung der Molekulargewichtsverteilung und spektroskopische Messungen nachgewiesen wurde.
    Notes: Redox polymerization of methylmethacrylate (MMA) using Ce(IV) with poly(oxyethylene) having azo and hydroxy functions was carried out to yield methylmethacrylate-ethylene glycol block copolymers with labile azo linkages in the main chain. These prepolymers were used to initiate the radical polymerization of styrene through the thermal decomposition of the azo group, resulting in the formation of multiblock copolymers. Successful blocking has been confirmed by fractional precipitation, a strong change in the molecular weight distribution and spectral measurements.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/apmc.1992.051950109
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