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  • 1
    Electronic Resource
    Electronic Resource
    Sensitization and photodynamic therapy of normal pancreas, duodenum and bile ducts in the hamster using 5-aminolaevulinic acid (1996)
    Springer
    Lasers in medical science 11 (1996), S. 11-21 
    Details
    ISSN: 1435-604X
    Keywords: Photodynamic therapy ; 5-Aminolaevulinic acid ; Pancreas ; Bile duct ; Duodenum ; Fluorescence microscopy ; Light dosimetry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract Photodynamic therapy (PDT) using 5-aminolaevulinic-acid-(ALA)-induced protoporphyrin IX (PPIX) increases survival in hamsters with pancreatic cancer. However, experiments with other photosensitizers on this model show a high risk of duodenal perforation. In this paper, the pharmacokinetics and PDT effects of ALA on normal tissues in the pancreatobiliary region are presented. Using quantitative fluorescence microscopy, maximum PPIX fluorescence was seen in the bile ducts, less in the duodenal mucosa and least in the muscularis propria and pancreas. For PDT, light was delivered either using a bare fibre touching the tissue (single-point illumination), or irradiating a 1.5 cm diameter circular area. Single-point PDT (50 J) produced only localized reversible damage without perforation. Surface irradiation of the whole periampullary region (50 J cm−2) caused extensive damage, sometimes with perforation. Before PDT can be used safely to treat tumours of the pancreas and bile duct, further studies are necessary to understand its effect on larger areas of normal tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02161288
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  • 2
    Electronic Resource
    Electronic Resource
    Infection of Spathoglottis plicata (Orchidaceae) seeds by mycorrhizal fungus (1998)
    Springer
    Plant cell reports 18 (1998), S. 14-19 
    Details
    ISSN: 1432-203X
    Keywords: Key words Artificial seed ; Orchid ; Mycorrhizal infection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Spathoglottis plicata seeds were encapsulated in 4-mm-diameter capsules of alginate-chitosan or alginate-gelatin and infected with the mycorrhizal fungus Rhizoctonia AM9. The encapsulated seeds were placed directly on Rhizoctonia culture. About 66% of the seeds encapsulated in sucrose-free chitosan-alginate established a symbiotic relationship with the mycorrhizal fungus after co-culturing for 2 weeks. The highest percentage of infection observed was about 84%. Addition of sucrose or using gelatin-alginate for encapsulation reduced the percentage of infection by about half. The growth of Rhizoctonia AM9 in sucrose-free alginate, chitosan and gelatin was found to be minimal. The advantages of germinating orchid seeds, encapsulated in sucrose-free polymers, through mycorrhizal infection is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050524
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  • 3
    Electronic Resource
    Electronic Resource
    Preliminary studies of micropropagation of Hopea odorata, a dipterocarp tree (1995)
    Springer
    Plant cell, tissue and organ culture 41 (1995), S. 193-196 
    Details
    ISSN: 1573-5044
    Keywords: Dipterocarpaceae ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plantlet development from in vitro cultures of Hopea odorato Roxb. is described. Embryos excised from seeds and cultured on Gamborg's B5 or modified Murashige and Skoog (MS) medium with benzyladenine (BA, 2.2–22.2 μM) produced axillary shoots at cotyledonary and/or stem nodes. Shoot production was greatest in germinated embryos on modified MS medium with 8.9 μM BA. Excised axillary shoots formed few buds when cultured on medium with BA and limited root development occurred on Woody Plant Medium with naphthaleneacetic acid. Nodal explants from aseptically grown plantlets sprouted axillary shoots in modified MS medium with BA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00051591
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  • 4
    Electronic Resource
    Electronic Resource
    Plantlet regeneration through different morphogenic pathways in pommelo tissue culture (1995)
    Springer
    Plant cell, tissue and organ culture 43 (1995), S. 301-303 
    Details
    ISSN: 1573-5044
    Keywords: Citrus grandis ; micropropagation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pommelo (Citrus grandis Osbeck) plantlets were regenerated through different morphogenic pathways in culture. Multiple shoot regeneration through de novo organogenesis was obtained with epicotyl segments and root cultures. Shoot regeneration was observed in 84% of the midtal epicotyl segments cultured in Murashige and Skoog's medium (MS) with 2.2 μM benzyladenine (BA) and 83% of the middle and proximal epicotyl segments cultured on basal medium. Isolated root segments cultured on medium containing 0.089 μM BA showed best shoot regeneration at 71% with an average of 3.3 shoots per segment. Callus tissues derived from cotyledon and leaf explants regenerated shoots on BA-enriched medium. Shoots were also obtained at high frequencies from shoot-tip and nodal explants. Roots developed when regenerated shoots were excised and cultured on half strength MS medium with 2.5 μM indolebutyric acid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039960
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  • 5
    Electronic Resource
    Electronic Resource
    A simple and efficient method for clonal propagation of Casuarina sumatrana (de Vriese) L. Johnson (1995)
    Springer
    Plant growth regulation 17 (1995), S. 115-120 
    Details
    ISSN: 1573-5087
    Keywords: auxin ; Casuarina sumatrana ; clonal propagation ; Neoflon film ; rooting ; trees
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A new and efficient method for clonal propagation of Casuarina sumatrana by rooting stem cuttings is described. High percentage (about 60–70%) of rooting was achieved with mature softwood stem cuttings. A quick-dip of 5 s in NAA (1–10mM) solution followed by sand culture under high humidity were required for a high rate of survival and rooting of stem cuttings. A simple, closed chamber propagation system, using fluorocarbon polymer (tetrafluoroethyleneperfluoroalkyl vinyl ether) film (Neoflon PFA film), was successfully developed for the rooting of stem cuttings without mist. Rooted cuttings inoculated with Frankia were easily transplanted and established in field conditions with very low (about 3%) mortality. The significance of these findings for mass clonal propagation of C. sumatrana is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024170
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